Symbiotic Functioning and Photosynthetic Rates Induced by Rhizobia Associated with Jack Bean (Canavalia ensiformis L.) Nodulation in Eswatini
Abstract
:1. Introduction
2. Materials and Methods
2.1. Origin of Isolates and Trapping Rhizobia in the Glasshouse
2.2. Bacteria Isolation from Root Nodules
2.3. Authentication of Bacterial Isolates
2.4. Gas Exchange Measurements and Symbiotic Effectiveness
2.5. Shoot and Root 15N/14N Analysis
2.6. Amount of N-Fixed in Shoots
2.7. Shoot and Root 13C/12C Isotopic Analysis
2.8. Statistical Analysis
3. Results
3.1. Nodule Number and Nodule Dry Matter per Plant
3.2. Shoot Dry Matter
3.3. Photosynthetic Rates, Stomatal Conductance, Leaf Transpiration and WUE Induced by Test Isolates
3.4. Relative Symbiotic Effectiveness
3.5. Symbiotic Parameters
3.5.1. Shoot %N, N Content, δ 15N and N-Fixed
3.5.2. Root %N, N Content, δ 15N and N-Fixed
3.5.3. Whole-Plant %N, N Content, δ 15N and N-Fixed
3.6. Carbon Assimilation and δ13C Values
3.6.1. Shoot %C, C Content, C:N Ratio and δ13C
3.6.2. Root %C, C Content, C:N Ratio and δ13C
4. Discussion
4.1. Symbiotic Effectiveness and Its Effect on Photosynthetic Rates Induced by Microsymbionts Nodulating Jack Bean in Eswatini Soils
4.2. Symbiotic Performance and C Assimilation by the 22 Microsymbionts Nodulating Jack Bean in Eswatini
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nutrient | Ka-Zulu Farm | New Heaven | Luve | Malkerns |
---|---|---|---|---|
pH (KCl) | 4.3 | 3.9 | 6.1 | 4.4 |
Total N (%) | 0.069 | 0.112 | 0.036 | 0.075 |
Available P (mg·kg−1) | 4 | 13 | 53 | 14 |
K (mg·kg−1) | 25 | 41 | 21 | 54 |
Cu (mg·kg−1) | 1.47 | 5.9 | 0.14 | 0.91 |
Zn (mg·kg−1) | 2.17 | 1.68 | 2.09 | 1.69 |
Ca (mg·kg−1) | 0.22 | 1.09 | 0.35 | 1.15 |
Fe (mg·kg−1) | 54.69 | 152.1 | 81.66 | 452.80 |
Mg cmol(+)Fe (mg·kg−1) | 0.13 | 0.81 | 0.18 | 0.84 |
Na (mg·kg−1) | 7 | 25 | 9 | 5 |
Isolate | Location | Nodule Number | Nodule Dry Matter | Nodule Dry Matter/Nodule Number | Shoot Dry Matter | Root Dry Matter | Root Dry Matter/Shoot Dry Matter | Whole-Plant Dry Matter | Relative Symbiotic Effectiveness |
---|---|---|---|---|---|---|---|---|---|
g.plant−1 | mg. plant−1 | g.plant−1. | g.plant−1 | g.plant−1 | g.plant−1 | % | |||
TUTCEeS1 | Malkerns | 194.00 ± 9.49 a | 0.20 ± 0.01 de | 1.04 ± 0.09 gh | 5.08 ± 0.12 bc | 0.60 ± 0.03 bc | 0.12 ± 0.00 g–k | 5.68 ± 0.14 bc | 109.55 ± 2.51 ab |
TUTCEeS2 | Malkerns | 194.00 ± 22.07 a | 0.24 ± 0.00 bc | 1.29 ± 0.16 fgh | 5.78 ± 0.32 b | 0.62 ± 0.02 b | 0.11 ± 0.01 ijk | 6.40 ± 0.33 b | 124.64 ± 6.85 a |
TUTCEeS3 | Malkerns | 126.00 ± 3.46 ef | 0.14 ± 0.01 gh | 1.09 ± 0.07 gh | 3.77 ± 0.38 gh | 0.57 ± 0.10 bcd | 0.16 ± 0.01 c–h | 4.33 ± 0.34 fg | 81.18 ± 8.12 ef |
TUTCEeS4 | Malkerns | 56.00 ± 2.65 g | 0.13 ± 0.01 ghi | 2.30 ± 0.28 c | 4.05 ± 0.05 efg | 0.60 ± 0.03 bc | 0.15 ± 0.01 d–j | 4.65 ± 0.03 def | 87.28 ± 1.08 de |
TUTCEeS5 | Malkerns | 195.00 ± 38.35 a | 0.20 ± 0.01 de | 1.09 ± 0.19 gh | 4.82 ± 0.15 c–f | 0.55 ± 0.05 bcd | 0.12 ± 0.01 g–k | 5.37 ± 0.10 cde | 103.81 ± 3.13 bcd |
TUTCEeS6 | Malkerns | 166.00 ± 6.33 a–d | 0.25 ± 0.01 b | 1.51 ± 0.20 efg | 4.87 ± 0.27 cd | 0.63 ± 0.02 b | 0.13 ± 0.00 f–k | 5.50 ± 0.28 c | 104.89 ± 5.78 bc |
TUTCEeS7 | Malkerns | 157.00 ± 6.89 b–e | 0.24 ± 0.01 bc | 1.52 ± 0.08 efg | 4.65 ± 0.14 c–f | 0.45 ± 0.03 cd | 0.10 ± 0.01 k | 5.10 ± 0.13 c–f | 100.22 ± 3.11 bcd |
TUTCEeS8 | Malkerns | 187.00 ± 6.51 ab | 0.15 ± 0.01 fg | 0.82 ± 0.05 h | 4.57 ± 0.34 c–f | 0.50 ± 0.03 bcd | 0.11 ± 0.00 h–k | 5.07 ± 0.37 c–f | 98.42 ± 7.26 bcd |
TUTCEeS9 | Malkerns | 141.00 ± 11.50 de | 0.12 ± 0.01 hi | 0.83 ± 0.03 h | 4.03 ± 0.24 fg | 0.57 ± 0.03 bcd | 0.14 ± 0.01 e–k | 4.60 ± 0.25 ef | 86.93 ± 5.18 de |
TUTCEeS10 | Malkerns | 20.00 ± 1.76 l–p | 0.06 ± 0.01 j | 3.23 ± 0.11 b | 2.88 ± 0.07 ij | 0.58 ± 0.04 bc | 0.20 ± 0.02 b–f | 3.47 ± 0.06 h | 62.14 ± 1.57 g |
TUTCEeS11 | Malkerns | 48.00 ± 3.38 gh | 0.11 ± 0.01 hi | 2.23 ± 0.05 cd | 2.63 ± 0.18 ij | 0.58 ± 0.02 bc | 0.22 ± 0.01 ab | 3.22 ± 0.19 h | 56.75 ± 3.95 g |
TUTCEeS12 | Malkerns | 149.00 ± 5.90 cde | 0.25 ± 0.01 b | 1.68 ± 0.02 d–g | 5.20 ± 0.20 bc | 0.52 ± 0.02 bcd | 0.10 ± 0.01 jk | 5.72 ± 0.19 bc | 112.07 ± 4.31 ab |
TUTCEeS13 | Malkerns | 164.00 ± 8.72 a–d | 0.21 ± 0.01 cd | 1.30 ± 0.13 fgh | 4.43 ± 0.14 c–g | 0.48 ± 0.04 bcd | 0.11 ± 0.01 h–k | 4.92 ± 0.09 c–f | 95.55 ± 2.94 b–e |
TUTCEeS14 | Malkerns | 181.00 ± 9.74 abc | 0.30 ± 0.01 a | 1.66 ± 0.09 d–g | 4.20 ± 0.15 d–g | 0.42 ± 0.02 d | 0.10 ± 0.00 jk | 4.62 ± 0.17 ef | 90.52 ± 3.29 cde |
TUTCEeS15 | Malkerns | 155.00 ± 4.04 b–e | 0.17 ± 0.01 ef | 1.11 ± 0.03 gh | 4.92 ± 0.12 cd | 0.60 ± 0.10 bc | 0.12 ± 0.02 g–k | 5.52 ± 0.04 c | 105.96 ± 2.51 bc |
TUTCEeS16 | Malkerns | 172.00 ± 14.19 a–d | 0.28 ± 0.01 a | 1.66 ± 0.06 d–g | 4.55 ± 0.43 c–f | 0.50 ± 0.07 bcd | 0.11 ± 0.02 g–k | 5.05 ± 0.47 c–f | 98.06 ± 9.29 bcd |
TUTCEeS17 | Malkerns | 20.00 ± 15.34 hi | 0.10 ± 0.01 i | 4.90 ± 0.49 a | 3.29 ± 0.43 hi | 0.50 ± 0.03 bcd | 0.16 ± 0.03 c–h | 3.79 ± 0.41 gh | 70.98 ± 9.32 fg |
TUTCEeS18 | Malkerns | 139.00 ± 8.69 de | 0.23 ± 0.01 bcd | 1.64 ± 0.11 d–g | 4.85 ± 0.13 cde | 0.61 ± 0.04 b | 0.13 ± 0.01 g–k | 5.46 ± 0.16 cd | 104.53 ± 2.71 bc |
TUTCEeS19 | Malkerns | 36.00 ± 1.20 hi | 0.11 ± 0.01 hi | 3.15 ± 0.19 b | 3.02 ± 0.34 hij | 0.60 ± 0.03 bc | 0.20 ± 0.01 bc | 3.62 ± 0.37 gh | 65.01 ± 7.37 fg |
TUTCEeS21 | Malkerns | 8.00 ± 0.67 i | 0.01 ± 0.00 k | 1.90 ± 0.48 c–f | 3.13 ± 0.09 hij | 0.55 ± 0.03 bcd | 0.18 ± 0.01 b–f | 3.68 ± 0.12 gh | 67.53 ± 2.00 fg |
TUTCEeS36 | New Heaven | 17.00 ± 1.73 hi | 0.03 ± 0.00 jk | 2.08 ± 0.12 cde | 2.53 ± 0.04 ij | 0.48 ± 0.03 bcd | 0.19 ± 0.01 bcd | 3.01 ± 0.07 h | 54.45 ± 0.93 g |
TUTCEeS38 | Luve | 56.00 ± 3.93 g | 0.20 ± 0.01 de | 3.58 ± 0.23 b | 3.23 ± 0.32 hij | 0.52 ± 0.03 bcd | 0.16 ± 0.01 c–g | 3.75 ± 0.35 gh | 69.68 ± 6.85 fg |
B. XS21 | - | 105.00 ± 0.88 f | 0.19 ± 0.02 de | 1.86 ± 0.11 c–f | 4.64 ± 0.08 c–f | 0.58 ± 0.02 bc | 0.13 ± 0.00 g–k | 5.23 ± 0.09 cde | 100.07 ± 1.70 bcd |
5 mM KNO3 | - | NA | NA | NA | 7.62 ± 0.31 a | 1.42 ± 0.07 a | 0.19 ± 0.00 b–e | 9.03 ± 0.38 a | NA |
Uninoculated (Control) | - | NA | NA | NA | 2.48 ± 0.26 j | 0.62 ± 0.02 b | 0.25 ± 0.02 a | 3.10 ± 0.28 h | NA |
F statistics | - | 55.29 *** | 17.89 *** | 12.39 *** | 21.42 *** | 13.67 *** | 11.13 *** | 21.87 *** | 15.55 *** |
Isolate | Photosynthic Rate | Stomatal Conductance | Transpiration Rate | Wate Use Efficiency |
---|---|---|---|---|
mol (CO2) m−2s−1 | mol (H2O) m−2s−1 | mol (H2O) m−2s−1 | µmol (CO2) mol−1 (H2O) | |
TUTCEeS1 | 12.96 ± 1.20 cd | 0.14 ± 0.02 c–g | 5.73 ± 0.83 b–e | 94.87 ± 9.11 bc |
TUTCEeS2 | 14.84 ± 0.06 b | 0.14 ± 0.00 c–e | 4.98 ± 0.00 def | 103.41 ± 0.33 ab |
TUTCEeS3 | 13.85 ± 0.47 b | 0.18 ± 0.01 de | 6.31 ± 0.22 bc | 63.16 ± 0.13 ij |
TUTCEeS4 | 11.62 ± 1.57 bc | 0.21 ± 0.06 b | 6.52 ± 1.23 b | 72.85 ± 10.67 ghi |
TUTCEeS5 | 12.86 ± 0.13 cd | 0.18 ± 0.00 bc | 6.61 ± 0.02 b | 73.44 ± 0.48 f–i |
TUTCEeS6 | ND | ND | ND | ND |
TUTCEeS7 | ND | ND | ND | ND |
TUTCEeS8 | 7.46 ± 0.05 g | 0.09 ± 0.00 ghi | 3.76 ± 0.01 ghi | 82.50 ± 0.36 c–g |
TUTCEeS9 | 13.31 ± 0.4 c | 0.15 ± 0.01 cde | 4.80 ± 0.17 efg | 91.35 ± 4.95 b–e |
TUTCEeS10 | 5.86 ± 0.12 h | 0.07 ± 0.00 hij | 3.30 ± 0.01 hi | 79.68 ± 1.73 d–g |
TUTCEeS11 | 18.81 ± 0.04 a | 0.30 ± 0.00 a | 9.30 ± 0.04 a | 62.45 ± 0.40 ij |
TUTCEeS12 | 8.68 ± 0.20 g | 0.09 ± 0.00 g–i | 4.20 ± 0.19 fgh | 93.70 ± 5.81 bcd |
TUTCEeS13 | 5.74 ± 5.74 hi | 0.07 ± 0.00 hij | 2.74 ± 0.00 i | 77.83 ± 0.87 e–h |
TUTCEeS14 | 15.00 ± 0.30 b | 0.18 ± 0.02 bc | 6.54 ± 0.47 b | 87.45 ± 8.57 c–f |
TUTCEeS15 | 10.56 ± 0.41 ef | 0.10 ± 0.01 e–i | 3.38 ± 0.23 hi | 103.25 ± 6.04 ab |
TUTCEeS16 | 12.63 ± 0.59 cd | 0.15 ± 0.02 cde | 5.23 ± 0.43 cf | 87.76 ± 7.07 c–f |
TUTCEeS17 | 7.46 ± 0.04 g | 0.12 ± 0.00 d–h | 4.89 ± 0.01 ef | 60.68 ± 0.28 ij |
TUTCEeS18 | 10.24 ± 0.03 jef | 0.11 ± 0.00 d–i | 4.78 ± 0.00 efg | 95.47 ± 0.25 bc |
TUTCEeS19 | 8.35 ± 0.02 g | 0.15 ± 0.00 cd | 5.47 ± 0.02 b–e | 56.12 ± 0.24 j |
TUTCEeS21 | ND | ND | ND | ND |
TUTCEeS36 | 4.32 ± 0.02 i | 0.04 ± 0.00 jk | 1.44 ± 0.01 j | 113.44 ± 0.49 a |
TUTCEeS38 | 4.50 ± 0.04 hi | 0.07 ± 0.00 ij | 2.82 ± 0.00 i | 65.09 ± 0.52 hij |
B. XS21 | 11.66 ± 0.04 de | 0.11 ± 0.00 d–i | 3.35 ± 0.00 hi | 110.64 ± 0.43 b |
5 mM KNO3 | 10.06 ± 0.09 f | 0.16 ± 0.00 cd | 6.04 ± 0.03 bcd | 64.01 ± 0.36 ij |
Uninoculated (Control) | 0.61 ± 0.08 j | 0.02 ± 0.00 k | 0.10 ± 0.18 j | 34.94 ± 4.06 k |
F statistics | 112.32 *** | 30.71 *** | 46.95 *** | 31.37 *** |
Isolate | Shoot | Root | Whole-Plant | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DM | N | N Content | δ15N | N-Fixed | DM | N | N Content | δ15N | N-Fixed | DM | N | N Content | δ15N | N-Fixed | |
g.plant−1 | % | mg.plant−1 | mg.plant−1 | g.plant−1 | % | mg.plant−1 | kg.ha−1 | g.plant−1 | % | mg.plant−1 | kg.ha−1 | ||||
TUTCEeS1 | 5.08 ± 0.12 bcd | 3.29 ± 0.18 abc | 167.61 ± 12.40 ab | −0.48 ± 0.17 e | 132.84 ± 10.24 abc | 0.60 ± 0.03 b | 2.55 ± 0.26 abc | 15.25 ± 1.44 bcd | 0.67 ± 0.14 l | 3.43 ± 0.56 ab | 5.68 ± 0.14 bcd | 5.84 ± 0.24 abc | 182.86 ± 12.65 ab | −0.38 ± 0.16 fg | 136.27 ± 10.03 abc |
TUTCEeS2 | 5.78 ± 0.32 b | 3.45 ± 0.14 ab | 200.47 ± 18.53 a | −0.37 ± 0.30 e | 165.70 ± 24.79 a | 0.60 ± 0.06 b | 2.42 ± 0.03 b–e | 14.53 ± 1.55 b–e | 2.05 ± 0.02 cd | 2.71 ± 3.04 ab | 6.38 ± 0.37 b | 5.87 ± 0.17 abc | 215.00 ± 20.01 a | −0.21 ± 0.28 efg | 168.40 ± 27.83 a |
TUTCEeS3 | 3.77 ± 0.38 fgh | 2.91 ± 0.26 abc | 111.40 ± 19.75 c–f | 0.13 ± 0.02 de | 76.62 ± 17.77 c–g | 0.57 ± 0.10 b | 2.52 ± 0.07 a–d | 14.28 ± 2.48 b–e | 1.64 ± 0.17 efg | 2.45 ± 2.56 ab | 4.33 ± 0.34 d–i | 5.43 ± 0.31 bcd | 125.67 ± 18.54 c–g | 0.31 ± 0.03 d–g | 79.08 ± 15.24 c–f |
TUTCEeS4 | 4.05 ± 0.05 efg | 2.63 ± 0.12 bcd | 106.48 ± 5.83 def | 0.58 ± 0.48 cde | 71.70 ± 5.30 d–h | 0.60 ± 0.03 b | 2.12 ± 0.01 fg | 12.72 ± 0.61 b–f | 2.28 ± 0.06 bc | 0.89 ± 2.30 ab | 4.18 ± 0.49 e–i | 4.75 ± 0.12 de | 119.19 ± 5.23 d–g | 0.76 ± 0.45 cde | 72.60 ± 6.47 d–g |
TUTCEeS5 | 4.82 ± 0.15 cde | 3.55 ± 0.14 ab | 170.49 ± 3.25 ab | 0.02 ± 0.22 e | 135.71 ± 4.82 abc | 0.55 ± 0.05 b | 2.37 ± 0.02 c–f | 13.03 ± 1.24 b–f | 1.30 ± 0.07 hij | 1.21 ± 2.50 ab | 5.37 ± 0.10 b–e | 5.91 ± 0.13 abc | 183.52 ± 3.23 ab | 0.11 ± 0.20 efg | 136.92 ± 6.80 abc |
TUTCEeS6 | 4.87 ± 0.27 cde | 3.93 ± 0.57 a | 191.61 ± 31.60 a | 0.13 ± 0.07 de | 156.83 ± 23.94 ab | 0.63 ± 0.07 b | 2.76 ± 0.08 a | 17.46 ± 1.95 b | 1.19 ± 0.20 ijk | 5.64 ± 0.96 a | 5.50 ± 0.33 b–e | 6.68 ± 0.64 a | 209.07 ± 33.27 ab | 0.22 ± 0.05 efg | 162.48 ± 23.73 ab |
TUTCEeS7 | 4.65 ± 0.14 c–f | 3.70 ± 0.15 a | 172.44 ± 12.41 ab | −0.57 ± 0.25 e | 137.67 ± 17.01 ab | 0.45 ± 0.03 b | 2.52 ± 0.02 a–d | 11.33 ± 0.67 c–f | 0.73 ± 0.07 l | −0.49 ± 2.14 ab | 5.10 ± 0.13 b–g | 6.22 ± 0.17 ab | 183.77 ± 12.03 ab | −0.49 ± 0.24 g | 137.17 ± 18.54 abc |
TUTCEeS8 | 4.57 ± 0.34 c–f | 2.98 ± 0.02 abc | 136.01 ± 10.89 b–e | −0.49 ± 0.11 e | 101.24 ± 17.18 b–f | 0.50 ± 0.03 b | 2.52 ± 0.12 a–d | 12.64 ± 1.08 b–f | 1.21 ± 0.07 ijk | 0.82 ± 1.85 ab | 5.07 ± 0.37 b–g | 5.50 ± 0.12 bcd | 148.65 ± 11.71 b–e | −0.34 ± 0.10 efg | 102.05 ± 19.02 b–e |
TUTCEeS9 | 4.03 ± 0.24 efg | 1.67 ± 0.26 de | 66.78 ± 8.75 fgh | 2.01 ± 0.71 ab | 32.01 ± 4.04 ghi | 0.57 ± 0.03 b | 2.44 ± 0.10 b–e | 13.86 ± 1.28 b–f | 1.74 ± 0.17 ef | 2.04 ± 3.09 ab | 4.93 ± 0.58 c–g | 4.11 ± 0.22 efg | 80.65 ± 7.53 ghi | 1.92 ± 0.59 ab | 34.05 ± 4.72 fgh |
TUTCEeS10 | 2.88 ± 0.07 i | 2.93 ± 0.19 abc | 84.27 ± 4.73 fgh | 0.61 ± 0.06 cde | 49.49 ± 4.46 f–i | 0.58 ± 0.07 b | 1.95 ± 0.01 ghi | 11.37 ± 1.28 c–f | 1.64 ± 0.04 efg | −0.45 ± 3.08 ab | 3.43 ± 0.17 hij | 4.88 ± 0.18 cde | 95.64 ± 4.09 f–i | 0.73 ± 0.08 c–f | 49.04 ± 7.50 f–h |
TUTCEeS11 | 2.63 ± 0.18 i | 1.89 ± 0.45 de | 51.33 ± 16.04 gh | 1.78 ± 0.72 abc | 16.56 ± 9.95 hi | 0.58 ± 0.02 b | 1.70 ± 0.09 hi | 9.90 ± 0.68 ef | 2.43 ± 0.03 b | −1.92 ± 1.25 ab | 3.38 ± 0.36 hij | 3.58 ± 0.53 g | 61.23 ± 16.71 hi | 1.86 ± 0.63 ab | 14.64 ± 9.29 gh |
TUTCEeS12 | 5.20 ± 0.20 bc | 3.36 ± 0.04 ab | 174.53 ± 5.13 ab | −0.42 ± 0.11 e | 139.76 ± 3.36 ab | 0.52 ± 0.02 b | 2.23 ± 0.14 ef | 11.50 ± 0.47 c–f | 1.65 ± 0.20 efg | −0.32 ± 1.36 ab | 5.75 ± 0.18 bc | 5.59 ± 0.12 bcd | 186.03 ± 5.59 ab | −0.29 ± 0.11 efg | 139.44 ± 4.58 abc |
TUTCEeS13 | 4.43 ± 0.14 c–f | 3.41 ± 0.15 ab | 151.53 ± 10.02 a–d | −0.13 ± 0.18 e | 116.75 ± 14.99 a–e | 0.50 ± 0.10 b | 2.54 ± 0.02 abc | 12.70 ± 2.66 b–f | 1.08 ± 0.06 jk | 0.88 ± 3.31 ab | 4.93 ± 0.55 c–g | 5.95 ± 0.16 ab | 164.23 ± 8.10 a–d | −0.04 ± 0.17 efg | 117.64 ± 15.97 a–d |
TUTCEeS14 | 4.20 ± 0.15 def | 3.81 ± 0.26 a | 159.35 ± 5.69 abc | −0.41 ± 0.24 e | 124.57 ± 11.32 a–d | 0.42 ± 0.04 b | 2.45 ± 0.01 b–e | 10.18 ± 1.02 def | 0.94 ± 0.01 kl | −1.64 ± 0.79 ab | 4.62 ± 0.36 c–h | 6.26 ± 0.27 ab | 169.53 ± 5.27 abc | −0.33 ± 0.23 efg | 122.94 ± 12.10 a–d |
TUTCEeS15 | 4.92 ± 0.12 cde | 3.14 ± 0.29 abc | 154.81 ± 17.73 a–d | −0.15 ± 0.28 e | 120.04 ± 24.24 a–d | 0.60 ± 0.10 b | 2.62 ± 0.05 abc | 15.80 ± 2.96 bc | 1.70 ± 0.02 efg | 3.98 ± 2.39 ab | 5.52 ± 0.04 b–e | 5.76 ± 0.24 abc | 170.61 ± 15.07 ab | 0.03 ± 0.27 efg | 124.02 ± 23.71 a–d |
TUTCEeS16 | 4.55 ± 0.43 c–f | 3.89 ± 0.56 a | 174.27 ± 18.57 ab | −0.16 ± 0.24 e | 139.49 ± 21.79 ab | 0.50 ± 0.07 b | 2.51 ± 0.12 a–d | 12.60 ± 1.89 b–f | 1.38 ± 0.16 g–j | 0.78 ± 1.38 ab | 5.05 ± 0.47 b–g | 6.40 ± 0.45 ab | 186.86 ± 19.93 abc | −0.06 ± 0.23 efg | 140.27 ± 23.17 abc |
TUTCEeS17 | 3.29 ± 0.43 ghi | 2.30 ± 0.41 cde | 76.67 ± 20.04 fgh | 0.63 ± 0.60 cde | 41.90 ± 27.79 ghi | 0.50 ± 0.03 b | 1.95 ± 0.04 ghi | 9.75 ± 0.44 ef | 1.81 ± 0.09 de | −2.07 ± 1.44 ab | 3.79 ± 0.41 g–j | 4.26 ± 0.37 efg | 86.43 ± 19.92 ghi | 0.75 ± 0.55 c–f | 39.84 ± 28.48 fgh |
TUTCEeS18 | 4.85 ± 0.13 cde | 3.46 ± 0.40 ab | 168.47 ± 22.48 ab | −0.22 ± 0.17 e | 131.16 ± 30.74 abc | 0.61 ± 0.04 b | 2.66 ± 0.05 ab | 16.26 ± 1.32 bc | 2.17 ± 0.11 bc | 4.44 ± 2.75 ab | 5.46 ± 0.56 b–e | 6.12 ± 0.45 ab | 184.73 ± 23.77 ab | −0.01 ± 0.15 efg | 135.60 ± 32.15 abc |
TUTCEeS19 | 3.02 ± 0.34 hi | 1.58 ± 0.07 e | 47.91 ± 6.74 gh | 1.30 ± 0.33 bcd | 13.14 ± 9.16 hi | 0.60 ± 0.03 b | 1.68 ± 0.02 i | 10.10 ± 0.57 def | 2.19 ± 0.07 bc | −1.72 ± 2.20 ab | 3.95 ± 0.70 f–j | 3.26 ± 0.08 g | 58.01 ± 7.21 hi | 1.45 ± 0.28 bc | 11.42 ± 11.25 gh |
TUTCEeS21 | 3.13 ± 0.09 hi | 1.63 ± 0.03 e | 51.13 ± 0.79 gh | 2.83 ± 0.03 a | 16.36 ± 8.48 hi | 0.53 ± 0.04 b | 1.81 ± 0.07 hi | 9.64 ± 0.67 ef | 2.95 ± 0.07 a | −2.18 ± 1.91 b | 3.33 ± 0.47 hij | 3.45 ± 0.09 g | 60.77 ± 1.43 hi | 2.85 ± 0.03 a | 14.18 ± 10.39 gh |
TUTCEeS36 | 2.53 ± 0.04 i | 1.73 ± 0.06 de | 43.62 ± 0.78 i | 2.67 ± 0.13 a | 9.64 ± 7.39 i | 0.48 ± 0.03 b | 1.96 ± 0.05 gh | 9.52 ± 0.88 ef | 2.98 ± 0.01 a | −2.30 ± 2.48 b | 2.80 ± 0.24 j | 3.69 ± 0.06 fg | 53.15 ± 0.87 i | 2.72 ± 0.10 a | 7.35 ± 9.86 h |
TUTCEeS38 | 3.23 ± 0.32 ghi | 2.89 ± 0.71 abc | 94.97 ± 29.15 efg | 0.42 ± 1.04 de | 60.20 ± 34.47 e–g | 0.52 ± 0.03 b | 1.73 ± 0.07 hi | 9.01 ± 0.92 f | 1.45 ± 0.06 f–i | −2.82 ± 2.69 b | 3.75 ± 0.35 g–j | 4.62 ± 0.68 def | 103.98 ± 29.21 e–h | 0.46 ± 0.94 c–g | 57.39 ± 35.79 e–h |
B. XS21 | 4.64 ± 0.08 c–f | 3.64 ± 0.45 ab | 168.76 ± 19.79 ab | −0.47 ± 0.25 e | 133.99 ± 22.27 abc | 0.58 ± 0.02 b | 2.25 ± 0.01 def | 13.13 ± 0.44 b–f | 1.57 ± 0.11 efg | 1.31 ± 1.77 ab | 5.23 ± 0.09 b–f | 5.89 ± 0.44 abc | 181.89 ± 19.36 ab | −0.31 ± 0.22 efg | 135.29 ± 23.13 abc |
Uninoculated (Control) | 2.48 ± 0.60 i | 1.40 ± 0.04 e | 34.77 ± 8.03 i | 1.25 ± 0.03 bcd | ND | 0.62 ± 0.10 b | 1.93 ± 0.07 ghi | 11.82 ± 1.81 c–f | 1.45 ± 0.04 f–i | ND | 3.10 ± 0.70 ij | 3.33 ± 0.05 g | 46.59 ± 9.80 i | 1.30 ± 0.02 bcd | ND |
5 mM KNO3 | 7.70 ± 040 a | 1.43 ± 0.13 e | 110.26 ± 12.02 def | 1.70 ± 0.04 abc | ND | 1.42 ± 0.19 a | 1.90 ± 0.05 ghi | 26.78 ± 2.85 a | 0.20 ± 0.02 m | ND | 9.12 ± 0.58 a | 3.33 ± 0.15 g | 137.04 ± 13.17 b–f | 1.41 ± 0.05 bc | ND |
F statistics | 19.78 *** | 7.82 *** | 12.31 *** | 7.77 *** | 8.16 *** | 7.40 *** | 16.36 *** | 5.78 *** | 42.43 *** | 2.50 ** | 10.34 *** | 13.01 *** | 12.60 *** | 8.75 *** | 7.71 *** |
Isolate | Shoot | Root | ||||||
---|---|---|---|---|---|---|---|---|
C | C Content | C:N Ratio | δ13C | C | C Content | C:N Ratio | δ13C | |
% | mg.plant−1 | ‰ | % | mg.plant−1 | ‰ | |||
TUTCEeS1 | 43.18 ± 0.0.16 abc | 2195.35 ± 58.07 bcd | 13.20 ± 0.67 e | −23.98 ± 0.06 ab | 44.73 ± 0.73 ab | 268.76 ± 16.91 b | −23.48 ± 0.08 bcd | 17.90 ± 1.90 fgh |
TUTCEeS2 | 43.24 ± 0.11 abc | 2500.50 ± 134.15 b | 12.57 ± 0.55 e | −24.59 ± 0.13 b–f | 42.70 ± 0.69 a–f | 255.52 ± 21.18 b | −24.01 ± 0.27 e–h | 17.68 ± 0.51 fgh |
TUTCEeS3 | 42.89 ± 0.07 abc | 1615.54 ± 160.71 fgh | 15.00 ± 1.47 de | −24.24 ± 0.18 a–d | 43.72 ± 0.94 abc | 249.49 ± 49.03 b | −24.13 ± 0.24 fgh | 17.36 ± 0.49 fgh |
TUTCEeS4 | 42.59 ± 0.39 c | 1725.10 ± 35.70 efg | 16.28 ± 0.73 de | −25.35 ± 0.20 g–j | 43.74 ± 0.18 abc | 262.52 ± 13.44 b | −23.62 ± 0.07 c–f | 20.64 ± 0.13 de |
TUTCEeS5 | 42.76 ± 0.11 bc | 2059.18 ± 57.21 cde | 12.09 ± 0.46 e | −24.31 ± 0.30 a–d | 42.92 ± 0.27 a–f | 236.08 ± 21.65 b | −23.10 ± 0.07 ab | 18.13 ± 0.23 fg |
TUTCEeS6 | 43.29 ± 0.14 abc | 2107.25 ± 119.07 cde | 11.44 ± 1.43 e | −24.23 ± 0.19 a–d | 43.43 ± 0.32 a–d | 275.25 ± 29.81 b | −23.11 ± 0.20 ab | 15.78 ± 0.33 h |
TUTCEeS7 | 42.44 ± 0.17 cd | 1973.29 ± 60.44 c–f | 11.51 ± 0.48 e | −24.60 ± 0.08 b–f | 42.58 ± 0.34 a–f | 191.80 ± 13.70 b | −23.31 ± 0.07 bc | 16.91 ± 0.27 fgh |
TUTCEeS8 | 43.20 ± 0.12 abc | 1972.79 ± 145.71 c–f | 14.52 ± 0.09 de | −23.87 ± 0.30 a | 45.06 ± 0.03 a | 225.33 ± 13.16 b | −23.56 ± 0.17 b–e | 17.95 ± 0.85 fgh |
TUTCEeS9 | 40.82 ± 0.28 ef | 1646.78 ± 99.89 fg | 25.49 ± 3.59 abc | −25.63 ± 0.16 h–k | 42.03 ± 1.99 b–f | 237.40 ± 12.74 b | −23.05 ± 0.12 ab | 17.30 ± 1.08 fgh |
TUTCEeS10 | 43.14 ± 0.06 abc | 1243.84 ± 32.35 i | 14.86 ± 0.96 de | −24.56 ± 0.23 b–f | 40.97 ± 0.67 c–g | 238.21 ± 24.48 b | −24.97 ± 0.09 i | 21.01 ± 0.27 d |
TUTCEeS11 | 40.99 ± 0.85 ef | 1082.07 ± 95.94 i | 23.73 ± 4.24 bc | −25.70 ± 0.19 ijk | 42.01 ± 0.42 b–f | 244.99 ± 6.61 b | −24.29 ± 0.11 h | 24.88 ± 1.07 a |
TUTCEeS12 | 43.88 ± 0.03 a | 2282.06 ± 89.20 bc | 13.07 ± 0.16 e | −24.47 ± 0.13 a–e | 39.02 ± 2.38 g | 200.81 ± 6.31 b | −23.51 ± 0.09 b–e | 17.49 ± 0.50 fgh |
TUTCEeS13 | 42.943 ± 0.19 abc | 1903.96 ± 61.73 c–f | 12.63 ± 0.50 e | −24.15 ± 0.15 a–d | 40.70 ± 1.32 d–g | 201.33 ± 37.63 b | −23.72 ± 0.12 c–g | 16.03 ± 0.61 gh |
TUTCEeS14 | 43.68 ± 0.32 ab | 1833.66 ± 53.01 def | 11.56 ± 0.72 e | −24.50 ± 0.09 b–e | 43.29 ± 0.26 a–d | 180.28 ± 18.72 b | −23.73 ± 0.11 c–g | 17.69 ± 0.09 fgh |
TUTCEeS15 | 42.80 ± 0.07 bc | 2104.23 ± 48.15 cde | 13.92 ± 1.42 de | −24.08 ± 0.17 abc | 43.62 ± 0.74 a–d | 263.24 ± 49.37 b | −23.99 ± 0.16 d–h | 16.65 ± 0.18 fgh |
TUTCEeS16 | 43.35 ± 0.26 abc | 1971.24 ± 181.74 c–f | 11.54 ± 1.41 e | −24.78 ± 0.23 d–g | 43.19 ± 0.16 a–e | 217.62 ± 32.30 b | −23.46 ± 0.26 bc | 17.29 ± 0.85 fgh |
TUTCEeS17 | 41.54 ± 0.46 de | 1368.99 ± 184.52 ghi | 19.77 ± 3.29 cd | −25.34 ± 0.20 g–j | 41.62 ± 0.49 c–g | 207.86 ± 10.24 b | −23.66 ± 0.14 c–g | 21.30 ± 0.188 cd |
TUTCEeS18 | 42.72 ± 0.04 bc | 2071.89 ± 55.57 cde | 12.64 ± 1.31 e | −24.52 ± 0.17 b–e | 42.77 ± 0.49 a–f | 261.24 ± 19.22 b | −22.73 ± 0.11 a | 16.08 ± 0.12 gh |
TUTCEeS19 | 40.51 ± 0.36 f | 1224.05 ± 149.12 i | 25.74 ± 1.20 abc | −25.61 ± 0.03 h–k | 40.24 ± 0.58 efg | 241.73 ± 14.91 b | −23.79 ± 0.24 c–g | 23.92 ± 0.34 a |
TUTCEeS21 | 40.76 ± 0.41 ef | 1277.70 ± 49.46 hi | 24.97 ± 0.68 abc | −25.85 ± 0.21 kj | 42.17 ± 0.08 a–f | 224.87 ± 18.27 b | −23.44 ± 0.08 bc | 23.33 ± 0.98 abc |
TUTCEeS36 | 41.55 ± 0.08 de | 1049.74 ± 18.17 i | 24.09 ± 0.80 bc | −25.51 ± 0.30 h–k | 40.06 ± 1.05 fg | 193.51 ± 13.35 b | −23.69 ± 0.16 c–g | 20.41 ± 0.66 de |
TUTCEeS38 | 42.37 ± 0.57 cd | 1372.30 ± 148.39 ghi | 17.08 ± 5.00 de | −25.16 ± 0.28 f–i | 41.15 ± 0.85 c–g | 213.16 ± 17.81 b | −23.70 ± 0.04 c–g | 23.77 ± 0.54 ab |
B. XS21 | 42.75 ± 0.25 bc | 1984.85 ± 34.03 c–f | 12.06 ± 1.28 e | −25.02 ± 0.02 e–h | 42.38 ± 0.86 a–f | 247.21 ± 9.03 b | −24.17 ± 0.22 gh | 18.83 ± 0.35 ef |
Uninoculated (Control) | 40.22 ± 0.19 f | 998.97 ± 242.14 i | 28.70 ± 0.94 abc | −26.08 ± 0.17 k | 41.96 ± 0.45 b–f | 258.20 ± 41.18 b | −24.86 ± 0.17 i | 21.79 ± 0.58 bcd |
5 mM KNO3 | 42.85 ± 0.15 bc | 3300.42 ± 179.91 a | 30.42 ± 2.56 a | −24.62 ± 0.17 c–f | 40.84 ± 0.45 c–g | 577.50 ± 73.28 a | −23.07 ± 0.11 ab | 21.48 ± 0.46 cd |
F statistics | 12.20 *** | 20.57 *** | 10.37 *** | 11.50 *** | 2.9 ** | 6.88 *** | 11.90 *** | 16.64 *** |
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Ngwenya, Z.D.; Dakora, F.D. Symbiotic Functioning and Photosynthetic Rates Induced by Rhizobia Associated with Jack Bean (Canavalia ensiformis L.) Nodulation in Eswatini. Microorganisms 2023, 11, 2786. https://doi.org/10.3390/microorganisms11112786
Ngwenya ZD, Dakora FD. Symbiotic Functioning and Photosynthetic Rates Induced by Rhizobia Associated with Jack Bean (Canavalia ensiformis L.) Nodulation in Eswatini. Microorganisms. 2023; 11(11):2786. https://doi.org/10.3390/microorganisms11112786
Chicago/Turabian StyleNgwenya, Zanele D., and Felix D. Dakora. 2023. "Symbiotic Functioning and Photosynthetic Rates Induced by Rhizobia Associated with Jack Bean (Canavalia ensiformis L.) Nodulation in Eswatini" Microorganisms 11, no. 11: 2786. https://doi.org/10.3390/microorganisms11112786