Microbial Biosurfactant: Candida bombicola as a Potential Remediator of Environments Contaminated by Heavy Metals
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganism
2.2. Substrates of the Production Medium
2.3. Inoculum Preparation
2.4. Biosurfactant Production
2.5. Determination of Surface Tension and Critical Micelle Concentration
2.6. Biosurfactant Isolation
2.7. Determination of the Emulsification Index
2.8. Stability Studies
2.9. Determination of Ionic Charge
2.10. Fourier-Transform Infrared Spectroscopy (FTIR)
2.11. Nuclear Magnetic Resonance Spectroscopy (NMR)
2.12. Phytotoxicity Assay
2.13. Toxicity Assay Using Eisenia Fetida
2.14. Collection of Soil Contaminated with Heavy Metals
2.15. Dynamic Treatment of Soils Contaminated by Heavy Metals
2.16. Static Treatment of Soils Contaminated by Heavy Metals
2.17. Kinetics of Heavy Metal Removal by Biosurfactant
2.18. Conductivity
3. Results and Discussion
3.1. Biosurfactant Production and Yield
3.2. Surface Tension and Critical Micelle Concentration
3.3. Biosurfactant Stability
3.4. Ionic Charge of the Biosurfactant
3.5. Fourier-Transform Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR)
3.6. Phytotoxicity Assay
3.7. Toxicity Assays Using Eisenia Fetida
3.8. Dynamic Treatment of Soil Contaminated by Heavy Metals
3.9. Static Treatment of Soil Contaminated by Heavy Metals
3.10. Kinetics of Heavy Metal Removal by Biosurfactant
3.11. Removal of Heavy Metals Contained in Synthetic Effluent by Biosurfactant
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Gaur, V.K.; Regar, R.K.; Dhiman, N.; Gautam, K.; Srivastava, J.K.; Patnaik, S.; Kamthan, M.; Manickam, N. Biosynthesis and characterization of sophorolipid biosurfactant by Candida spp.: Application as food emulsifier and antibacterial agent. Bioresour. Technol. 2019, 285, 121314. [Google Scholar] [CrossRef] [PubMed]
- Almeida, D.G.; Soares da Silva, R.D.C.F.; Meira, H.M.; Brasileiro, P.P.F.; Silva, E.J.; Luna, J.M.; Rufino, R.D.; Sarubbo, L.A. Production, characterization and commercial formulation of a biosurfactant from Candida tropicalis UCP0996 and its application in decontamination of petroleum pollutants. Processes 2021, 9, 885. [Google Scholar] [CrossRef]
- Santos, E.M.S.; Lira, I.R.A.S.; Meira, H.M.; Aguiar, J.S.; Rufino, R.D.; Almeida, D.G.; Casazza, A.A.; Converti, A.; Sarubbo, L.A.; Luna, J.M. Enhanced oil removal by a non-toxic biosurfactant formulation. Energies 2021, 14, 467. [Google Scholar] [CrossRef]
- Akbari, S.; Abdurahman, N.H.; Yunus, R.M.; Fayaz, F.; Alara, O.R. Biosurfactants—A new frontier for social and environmental safety: A mini review. Biotechnol. Res. Innov. 2018, 2, 81–90. [Google Scholar] [CrossRef]
- Dhaliwal, S.S.; Singh, J.; Taneja, P.K.; Mandal, A. Remediation techniques for removal of heavy metals from the soil contaminated through different sources: A review. Environ. Sci. Pollut. Res. 2020, 27, 1319–1333. [Google Scholar] [CrossRef] [PubMed]
- Ali, H.; Khan, E.; Ilahi, I. Environmental chemistry and ecotoxicology of hazardous heavy metals: Environmental persistence, toxicity, and bioaccumulation. J. Chem. 2019, 2019, 6730305. [Google Scholar] [CrossRef]
- Rastogi, S.; Kumar, R. Remediation of heavy metals using non-conventional adsorbents and biosurfactant-producing bacteria. Environ. Degrad. Causes Remediat. Strateg. 2020, 1, 23–45. [Google Scholar] [CrossRef]
- Chen, Q.; Li, Y.; Liu, M.; Zhu, B.; Mu, J.; Chen, Z. Removal of Pb and Hg from marine intertidal sediment by using rhamnolipid biosurfactant produced by a Pseudomonas aeruginosa strain. Environ. Technol. Innov. 2021, 22, 101456. [Google Scholar] [CrossRef]
- Rahman, Z.; Singh, V.P. Bioremediation of toxic heavy metals (THMs) contaminated sites: Concepts, applications and challenges. Environ. Sci. Pollut. Res. 2020, 27, 27563–27581. [Google Scholar] [CrossRef]
- Li, C.; Zhou, K.; Qin, W.; Tian, C.; Qi, M.; Yan, X.; Han, W. A review on heavy metals contamination in soil: Effects, sources, and remediation techniques. Soil Sediment Contam. Int. J. 2019, 28, 380–394. [Google Scholar] [CrossRef]
- Kumar, S.; Prasad, S.; Yadav, K.K.; Shrivastava, M.; Gupta, N.; Nagar, S.; Malav, L.C. Hazardous heavy metals contamination of vegetables and food chain: Role of sustainable remediation approaches-A review. Environ. Res. 2019, 179, 108792. [Google Scholar] [CrossRef] [PubMed]
- CWC (Central Water Commission). Status of Trace and Toxic Metals in Indian Rivers; Government of India, Ministry of Water Resources, River Development and Ganga Rejuvenation; Central Water Commission; River Data Compilation-2 Directorate Planning and Development Organization: Atlanta, GA, USA, 2018.
- Jaishankar, M.; Tseten, T.; Anbalagan, N.; Mathew, B.B.; Beeregowda, K.N. Toxicity, mechanism and health effects of some heavy metals. Interdiscip. Toxicol. 2014, 7, 60. [Google Scholar] [CrossRef] [PubMed]
- Yadav, K.K.; Gupta, N.; Kumar, V.; Choudhary, P.; Khan, S.A. GIS-based evaluation of groundwater geochemistry and statistical determination of the fate of contaminants in shallow aquifers from different functional areas of Agra city, India: Levels and spatial distributions. RSC Adv. 2018, 8, 15876–15889. [Google Scholar] [CrossRef] [PubMed]
- Maurya, P.K.; Malik, D.S.; Yadav, K.K.; Kumar, A.; Kumar, S.; Kamyab, H. Bioaccumulation and potential sources of heavy metal contamination in fish species in River Ganga basin: Possible human health risks evaluation. Toxicol. Rep. 2019, 6, 472–481. [Google Scholar] [CrossRef]
- Singh, S.; Kumar, V.; Singh, S.; Dhanjal, D.S.; Datta, S.; Sharma, D.; Singh, N.K.; Singh, J. Biosurfactant-based bioremediation. In Bioremediation of Pollutants; Elsevier: Amsterdam, The Netherlands, 2020; pp. 333–358. [Google Scholar] [CrossRef]
- Luna, J.M.; Rufino, R.D.; Sarubbo, L.A.; Campos-Takaki, G.M. Characterization, surface properties and biological activity of a biosurfactant produced from industrial waste by Candida sphaerica UCP0995 for application in the petroleum industry. Colloids Surf. B Biointerfaces 2013, 102, 202–209. [Google Scholar] [CrossRef]
- Silva, S.N.R.L.; Farias, C.B.B.; Rufino, R.D.; Luna, J.M.; Sarubbo, L.A. Glycerol as substrate for the production of biosurfactant by Pseudomonas aeruginosa UCP0992. Colloids Surf. B Biointerfaces 2010, 79, 174–183. [Google Scholar] [CrossRef]
- Cooper, D.G.; Goldenberg, B.G. Surface-active agents from two Bacillus species. Appl. Environ. Microbiol. 1987, 53, 224–229. [Google Scholar] [CrossRef]
- Tiquia, S.M.; Tam, N.F.Y.; Hodgkiss, I.J. Effects of composting on phytotoxicity of spent pig-manure sawdust litter. Environ. Pollut. 1996, 93, 249–256. [Google Scholar] [CrossRef]
- Andréa, M.M.D. O uso de minhocas como bioindicadores de contaminação de solos. Acta Zoológica Mex. 2010, 26, 95–107. [Google Scholar] [CrossRef]
- ABNT NBR 17512/2011—Qualidade do Solo. Ensaio de Fuga Para Avaliar a Qualidade de Solos e Efeitos de Substâncias Químicas no Comportamento Parte 1: Ensaio com Minhocas (Eisenia fetida e Eisenia andrei), 2nd ed.; Coletânea Normas Ténicas Ecotoxicologia—Terrestres: Rio de Janeiro, Brazil, 2019; p. 10. [Google Scholar]
- Luna, J.M.; Rufino, R.D.; Sarubbo, L.A. Biosurfactant from Candida sphaerica UCP0995 exhibiting heavy metal remediation properties. Process Saf. Environ. Prot. 2016, 102, 558–566. [Google Scholar] [CrossRef]
- Rufino, R.D.; Luna, J.M.; Marinho, P.H.C.; Farias, C.B.B.; Ferreira, S.R.M.; Sarubbo, L.A. Removal of petroleum derivative adsorbed to soil by biosurfactant Rufisan produced by Candida lipolytica. J. Pet. Sci. Eng. 2013, 109, 117–122. [Google Scholar] [CrossRef]
- Rocha Junior, R.B.; Meira, H.M.; Almeida, D.G.; Rufino, R.D.; Luna, J.M.; Santos, V.A.; Sarubbo, L.A. Application of a low-cost biosurfactant in heavy metal remediation processes. Biodegradation 2019, 30, 215–233. [Google Scholar] [CrossRef] [PubMed]
- Das, P.; Mukherjee, S.; Sen, R. Biosurfactant of marine origin exhibiting heavy metal remediation properties. Bioresour. Technol. 2009, 100, 4887–4890. [Google Scholar] [CrossRef] [PubMed]
- López-Prieto, A.; Rodríguez-López, L.; Rincón-Fontán, M.; Cruz, J.M.; Moldes, A.B. Characterization of extracellular and cell bound biosurfactants produced by Aneurinibacillus aneurinilyticus isolated from commercial corn steep liquor. Microbiol. Res. 2021, 242, 126614. [Google Scholar] [CrossRef]
- Wu, W.; Pang, B.; Yang, R.; Liu, G.; Ai, C.; Jiang, C.; Shi, J. Improvement of the probiotic potential and yield of Lactobacillus rhamnosus cells using corn steep liquor. LWT 2020, 131, 109862. [Google Scholar] [CrossRef]
- Jamir, L.; Kumar, V.; Kaur, J.; Kumar, S.; Singh, H. Composition, valorization and therapeutical potential of molasses: A critical review. Environ. Technol. Rev. 2021, 10, 131–142. [Google Scholar] [CrossRef]
- Fraga, J.L.; da Silva Pereira, A.; Diniz, M.M.; Fickers, P.; Amaral, P.F. Valorization of urban waste oil by microbial conversions. Case Stud. Chem. Environ. Eng. 2021, 4, 100145. [Google Scholar] [CrossRef]
- Ferreira, I.N.S.; Rodríguez, D.M.; Campos-Takaki, G.M.; da Silva Andrade, R.F. Biosurfactant and bioemulsifier as promising molecules produced by Mucor hiemalis isolated from Caatinga soil. Electron. J. Biotechnol. 2020, 47, 51–58. [Google Scholar] [CrossRef]
- Asgher, M.; Afzal, M.; Qamar, S.A.; Khalid, N. Optimization of biosurfactant production from chemically mutated strain of Bacillus subtilis using waste automobile oil as low-cost substrate. Environ. Sustain. 2020, 3, 405–413. [Google Scholar] [CrossRef]
- Lira, I.R.A.S.; Santos, E.M.S.; Selva Filho, A.A.; Farias, C.B.B.; Guerra, J.M.C.; Sarubbo, L.A.; Luna, J.M. Biosurfactant Production from Candida guilliermondii and Evaluation of its Toxicity. CET J.-Chem. Eng. Trans. 2020, 79, 457–462. [Google Scholar] [CrossRef]
- Silva, I.A.; Veras, B.O.; Ribeiro, B.G.; Aguiar, J.S.; Guerra, J.M.C.; Luna, J.M.; Sarubbo, L.A. Production of cupcake-like dessert containing microbial biosurfactant as an emulsifier. PeerJ 2020, 8, e9064. [Google Scholar] [CrossRef]
- Ribeiro, B.G.; Guerra, J.M.C.; Sarubbo, L.A. Potential food application of a biosurfactant produced by Saccharomyces cerevisiae URM 6670. Front. Bioeng. Biotechnol. 2020, 8, 434. [Google Scholar] [CrossRef] [PubMed]
- Silva, I.G.S.; de Almeida, F.C.G.; da Rocha e Silva, N.M.P.; de Oliveira, J.T.R.; Converti, A.; Sarubbo, L.A. Application of green surfactants in the remediation of soils contaminated by hydrocarbons. Processes 2021, 9, 1666. [Google Scholar] [CrossRef]
- Santos, J.C.V.; Santos, E.M.S.; Silva, Y.A.; Lira, I.R.A.S.; Silva, R.R.; Durval, I.J.B.; Sarubbo, L.A.; Luna, J.M. Application of Candida lipolytica biosurfactant for bioremediation of motor oil from contaminated environment. Chem. Eng. Trans. 2021, 86, 649–654. [Google Scholar] [CrossRef]
- Markande, A.R.; Patel, D.; Varjani, S. A review on biosurfactants: Properties, applications and current developments. Bioresour. Technol. 2021, 330, 124963. [Google Scholar] [CrossRef]
- Ribeiro, B.G.; dos Santos, M.M.; Pinto, M.I.; Meira, H.M.; Durval, I.J.; Guerra, J.M.; Sarubbo, L.A. Production and Optimization of the Extraction Conditions of the Biosurfactant from Candida Utilis UFPEDA1009 with Potential Application in the Food Industry. CET J.-Chem. Eng. Trans. 2019, 74, 1477–1482. [Google Scholar] [CrossRef]
- Radha, P.; Suhazsini, P.; Prabhu, K.; Jayakumar, A.; Kandasamy, R. Chicken tallow, a renewable source for the production of biosurfactant by Yarrowia lipolytica MTCC9520, and its application in silver nanoparticle synthesis. J. Surfactants Deterg. 2020, 23, 119–135. [Google Scholar] [CrossRef]
- Kumar, A.; Singh, S.K.; Kant, C.; Verma, H.; Kumar, D.; Singh, P.P.; Modi, A.; Droby, S.; Kesawat, M.S.; Alavilli, H.; et al. Microbial biosurfactant: A new frontier for sustainable agriculture and pharmaceutical industries. Antioxidants 2021, 10, 1472. [Google Scholar] [CrossRef]
- Twigg, M.S.; Baccile, N.; Banat, I.M.; Déziel, E.; Marchant, R.; Roelants, S.; Van Bogaert, I.N. Microbial biosurfactant research: Time to improve the rigour in the reporting of synthesis, functional characterization and process development. Microb. Biotechnol. 2021, 14, 147–170. [Google Scholar] [CrossRef]
- Ambaye, T.G.; Vaccari, M.; Prasad, S.; Rtimi, S. Preparation, characterization and application of biosurfactant in various industries: A critical review on progress, challenges and perspectives. Environ. Technol. Innov. 2021, 24, 102090. [Google Scholar] [CrossRef]
- Nogueira, I.B.; Rodríguez, D.M.; da Silva Andradade, R.F.; Lins, A.B.; Bione, A.P.; da Silva, I.G.S.; Franco, L.O.; de Campos-Takaki, G.M. Bioconversion of agroindustrial waste in the production of bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and application in bioremediation process. Int. J. Chem. Eng. 2020, 2020, 9434059. [Google Scholar] [CrossRef]
- Durval, I.J.B.; Resende, A.H.M.; Figueiredo, M.A.; Luna, J.M.; Rufino, R.D.; Sarubbo, L.A. Studies on biosurfactants produced using Bacillus cereus isolated from seawater with biotechnological potential for marine oil-spill bioremediation. J. Surfactants Deterg. 2019, 22, 349–363. [Google Scholar] [CrossRef]
- Sobrinho, H.B.; Rufino, R.D.; Luna, J.M.; Salgueiro, A.A.; Campos-Takaki, G.M.; Leite, L.F.; Sarubbo, L.A. Utilization of two agroindustrial by-products for the production of a surfactant by Candida sphaerica UCP0995. Process Biochem. 2008, 43, 912–917. [Google Scholar] [CrossRef]
- Werrie, P.Y.; Durenne, B.; Delaplace, P.; Fauconnier, M.L. Phytotoxicity of essential oils: Opportunities and constraints for the development of biopesticides. A review. Foods 2020, 9, 1291. [Google Scholar] [CrossRef] [PubMed]
- Santos, D.K.F.; Meira, H.M.; Rufino, R.D.; Luna, J.M.; Sarubbo, L.A. Biosurfactant production from Candida lipolytica in bioreactor and evaluation of its toxicity for application as a bioremediation agent. Process Biochem. 2017, 54, 20–27. [Google Scholar] [CrossRef]
- Felix, A.K.N.; Martins, J.J.; Almeida, J.G.L.; Giro, M.E.A.; Cavalcante, K.F.; Melo, V.M.M.; Pessoa, O.D.L.; Rocha, M.V.P.; Gonçalves, L.R.B.; de Santiago Aguiar, R.S. Purification and characterization of a biosurfactant produced by Bacillus subtilis in cashew apple juice and its application in the remediation of oil-contaminated soil. Colloids Surf. B Biointerfaces 2019, 175, 256–263. [Google Scholar] [CrossRef]
- Li, Y.; Wang, J.; Shao, M.A. Assessment of earthworms as an indicator of soil degradation: A case-study on loess soils. Land Degrad. Dev. 2021, 32, 2606–2617. [Google Scholar] [CrossRef]
- Sanchez-Hernandez, J.C.; Ríos, J.M.; Attademo, A.M.; Malcevschi, A.; Cares, X.A. Assessing biochar impact on earthworms: Implications for soil quality promotion. J. Hazard. Mater. 2019, 366, 582–591. [Google Scholar] [CrossRef]
- Khan, M.A.I.; Biswas, B.; Smith, E.; Naidu, R.; Megharaj, M. Toxicity assessment of fresh and weathered petroleum hydrocarbons in contaminated soil—A review. Chemosphere 2018, 212, 755–767. [Google Scholar] [CrossRef]
- Soroldoni, S.; Silva, G.; Correia, F.V.; Marques, M. Spent lubricant oil-contaminated soil toxicity to Eisenia andrei before and after bioremediation. Ecotoxicology 2019, 28, 212–221. [Google Scholar] [CrossRef]
- Mishra, S.; Lin, Z.; Pang, S.; Zhang, Y.; Bhatt, P.; Chen, S. Biosurfactant is a powerful tool for the bioremediation of heavy metals from contaminated soils. J. Hazard. Mater. 2021, 418, 126253. [Google Scholar] [CrossRef] [PubMed]
- Ravindran, A.; Sajayan, A.; Priyadharshini, G.B.; Selvin, J.; Kiran, G.S. Revealing the efficacy of thermostable biosurfactant in heavy metal bioremediation and surface treatment in vegetables. Front. Microbiol. 2020, 11, 222. [Google Scholar] [CrossRef] [PubMed]
- Mulligan, C.N. Sustainable remediation of contaminated soil using biosurfactants. Front. Bioeng. Biotechnol. 2021, 9, 635196. [Google Scholar] [CrossRef]
- Lal, S.; Ratna, S.; Said, O.B.; Kumar, R. Biosurfactant and exopolysaccharide-assisted rhizobacterial technique for the remediation of heavy metal contaminated soil: An advancement in metal phytoremediation technology. Environ. Technol. Innov. 2018, 10, 243–263. [Google Scholar] [CrossRef]
- Almeida, D.G.; Soares da Silva, R.D.C.F.; Brasileiro, P.P.F.; Rufino, R.D.; de Luna, J.M.; Sarubbo, L.A. Production, formulation and cost estimation of a commercial biosurfactant. Biodegradation 2019, 30, 191–201. [Google Scholar] [CrossRef]
- Sarubbo, L.; Brasileiro, P.; Silveira, G.; Luna, J.; Rufino, R. Application of a low cost biosurfactant in the removal of heavy metals in soil. Chem. Eng. Trans. 2018, 64, 433–438. [Google Scholar] [CrossRef]
- Narimannejad, S.; Zhang, B.; Lye, L. Adsorption behavior of cobalt onto saline soil with/without a biosurfactant: Kinetic and isotherm studies. Water Air Soil Pollut. 2019, 230, 47. [Google Scholar] [CrossRef]
- Jia, K.; Yi, Y.; Ma, W.; Cao, Y.; Li, G.; Liu, S.; Wang, T.; An, N. Ion flotation of heavy metal ions by using biodegradable biosurfactant as collector: Application and removal mechanism. Miner. Eng. 2022, 176, 107338. [Google Scholar] [CrossRef]
- Ayangbenro, A.S.; Babalola, O.O. Metal (loid) bioremediation: Strategies employed by microbial polymers. Sustainability 2018, 10, 3028. [Google Scholar] [CrossRef]
- Tang, J.; He, J.; Tang, H.; Wang, H.; Sima, W.; Liang, C.; Qiu, Z. Heavy metal removal effectiveness, flow direction and speciation variations in the sludge during the biosurfactant-enhanced electrokinetic remediation. Sep. Purif. Technol. 2020, 246, 116918. [Google Scholar] [CrossRef]
NaCl (%) | Surface Tension (mN/m) | Temperature (°C) | Surface Tension (mN/m) | pH | Surface Tension (mN/m) |
---|---|---|---|---|---|
0 | 29 ± 1.3 | 0 | 30 ± 1.1 | 0 | 29 ± 1.3 |
2 | 30 ± 1.3 | 5 | 30 ± 1.2 | 2 | 35 ± 1.1 |
4 | 30 ± 1.8 | 70 | 29 ± 2.0 | 4 | 34 ± 1.2 |
6 | 30 ± 1.2 | 100 | 31 ± 1.2 | 6 | 30 ± 1.2 |
8 | 30 ± 1.4 | 120 | 29 ± 1.3 | 8 | 28 ± 1.3 |
10 | 31 ± 1.1 | 10 | 31 ± 1.2 | ||
12 | 31 ± 1.2 | 12 | 33 ± 1.2 |
NaCl (%) | Engine Oil Emulsification (%) | Temperature (°C) | Engine Oil Emulsification (%) | pH | Engine Oil Emulsification (%) |
---|---|---|---|---|---|
0 | 90 ± 1.2 | 0 | 100 ± 1.1 | 0 | 90 ± 1.2 |
2 | 89 ± 1.3 | 5 | 100 ± 1.3 | 2 | 86 ± 1.1 |
4 | 89 ± 1.8 | 70 | 100 ± 1.2 | 4 | 90 ± 1.7 |
6 | 89 ± 1.2 | 100 | 100 ± 1.2 | 6 | 92 ± 1.2 |
8 | 89 ± 1.4 | 120 | 100 ± 1.3 | 8 | 89 ± 1.5 |
10 | 84 ± 1.1 | 10 | 94 ± 1.2 | ||
12 | 87 ± 1.2 | 12 | 95 ± 1.2 |
Treatments | Removal (%) | ||
---|---|---|---|
Pb | Zn | Fe | |
Distilled water (control) | 10 ± 1.0 | 15 ± 1.1 | 12 ± 1.3 |
Cell-free metabolic fluid | 48 ± 1.2 | 71 ± 1.5 | 88 ± 1.4 |
0.25% biosurfactant solution (½ CMC) | 25 ± 1.4 | 65 ± 1.2 | 78 ± 1.4 |
0.5% biosurfactant solution (1CMC) | 33 ± 2.1 | 68 ± 1.7 | 84 ± 1.2 |
1% biosurfactant solution (2CMC) | 45 ± 1.4 | 70 ± 1.2 | 80 ± 2.0 |
Treatments | Removal (%) | ||
---|---|---|---|
Pb | Zn | Fe | |
Distilled water (control) | 5 ± 1.0 | 11 ± 1.1 | 13 ± 1.3 |
Cell-free metabolic fluid | 37 ± 1.2 | 58 ± 1.2 | 60 ± 1.3 |
0.25% biosurfactant solution (½ CMC) | 30 ± 1.1 | 51 ± 1.2 | 57 ± 1.6 |
0.5% biosurfactant solution (1CMC) | 35 ± 1.3 | 56 ± 1.7 | 61 ± 1.3 |
1% biosurfactant solution (2CMC) | 40 ± 1.1 | 60 ± 1.2 | 65 ± 1.0 |
Heavy Metal | Conductivity of Metallic Solutions (µS/Cm) | Conductivity of the Solution after Addition of the Biosurfactant | ||
---|---|---|---|---|
½ CMC | 1CMC | 2CMC | ||
Pb | 670.3 | 382 ± 1.2 | 387 ± 1.1 | 407 ± 1.6 |
Cd | 512.4 | 360 ± 1.4 | 366 ± 1.2 | 392 ± 1.1 |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
da Silva, R.R.; Santos, J.C.V.; Meira, H.M.; Almeida, S.M.; Sarubbo, L.A.; Luna, J.M. Microbial Biosurfactant: Candida bombicola as a Potential Remediator of Environments Contaminated by Heavy Metals. Microorganisms 2023, 11, 2772. https://doi.org/10.3390/microorganisms11112772
da Silva RR, Santos JCV, Meira HM, Almeida SM, Sarubbo LA, Luna JM. Microbial Biosurfactant: Candida bombicola as a Potential Remediator of Environments Contaminated by Heavy Metals. Microorganisms. 2023; 11(11):2772. https://doi.org/10.3390/microorganisms11112772
Chicago/Turabian Styleda Silva, Renata Raianny, Júlio C. V. Santos, Hugo M. Meira, Sérgio M. Almeida, Leonie A. Sarubbo, and Juliana M. Luna. 2023. "Microbial Biosurfactant: Candida bombicola as a Potential Remediator of Environments Contaminated by Heavy Metals" Microorganisms 11, no. 11: 2772. https://doi.org/10.3390/microorganisms11112772