A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria
Abstract
:1. Introduction
2. Methods
2.1. Preparation of Stool Samples
2.2. Bacterial Strains
2.3. In Vitro Evaluation of the Anti-XDR Bacteria Growth Activity of Human Fecal Microbiota
2.4. Evaluation of the Inhibitory Effect of FMT in an XDR Intestinal Carriage Mouse Model
2.5. Monitoring of XDR Intestinal Carriage
2.6. Statistical Analysis
3. Results
3.1. In Vitro Agar Spot Test for Measurement of the Anti-XDR Bacteria Activity of Stool Samples
3.2. Efficacy of FMT Performed with Selected Fecal Samples on the Intestinal Carriage of XDR in Mice
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Salandre, A.; Delannoy, J.; Goudiaby, M.T.B.; Barbut, F.; Thomas, M.; Waligora-Dupriet, A.-J.; Kapel, N. A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria. Microorganisms 2023, 11, 2753. https://doi.org/10.3390/microorganisms11112753
Salandre A, Delannoy J, Goudiaby MTB, Barbut F, Thomas M, Waligora-Dupriet A-J, Kapel N. A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria. Microorganisms. 2023; 11(11):2753. https://doi.org/10.3390/microorganisms11112753
Chicago/Turabian StyleSalandre, Angélique, Johanne Delannoy, Marie Thérèse Barba Goudiaby, Frédéric Barbut, Muriel Thomas, Anne-Judith Waligora-Dupriet, and Nathalie Kapel. 2023. "A Simple In Vitro Test to Select Stools for Fecal Microbiota Transplantation to Limit Intestinal Carriage of Extensively Drug-Resistant Bacteria" Microorganisms 11, no. 11: 2753. https://doi.org/10.3390/microorganisms11112753