Shewanella sp. T2.3D-1.1 a Novel Microorganism Sustaining the Iron Cycle in the Deep Subsurface of the Iberian Pyrite Belt
Round 1
Reviewer 1 Report
I enjoy reading your interestimng contribution, I just made a few comments on minor mistakes in paragraphs.
Comments for author File: Comments.pdf
Author Response
I have activated the "Track changes" option from word so that all changes can be followed easily. All the comments submitted by the reviewers have been addressed and corrected accordingly.
Comment 1: "of" corrected accordingly in the text
Comment 2: "space" corrected accordingly in the text
Comment 3: "space" corrected accordingly in the text
Comment 4: "is this symbol OK? add space" the symbol is correct and the space has been corrected in the text.
Comment 5: "space" corrected accordingly in the text
Comment 6: "delete add" corrected accordingly in the text
Comment 7: "delete extra space" corrected accordingly in the text
Comment 8: "delete extra space" corrected accordingly in the text
Comment 9: "delete extra space" corrected accordingly in the text
Comment 10: "continue paragraph" corrected accordingly in the text
Comment 11: "delete exta space" corrected accordingly in the text
Comment 12: "extra space" corrected accordingly in the text
Comment 13: "please revise this sentence, its not clear", I have added "is lower" to make more sense of the sentence and removed acidic from the sentence since the isolates that were compared to are from marine water enviroment.
Comment 14: "extra space" corrected accordingly in the text
Comments 15 & 16 : "Caption for this table is in the second reference" & "Please check this" I have moved the table to the next page and the caption has been also moved so that it is located below the table.
Reviewer 2 Report
The manuscript presents a very interesting study regarding a new bacterial strain, Shewanella sp. T2.3D-1.1, involved in the iron cycle from the largest deposits of sulphide minerals on Earth, namely the Iberian Pyrite Belt. The isolation of this bacterium, genomic and phenotypic characterization, various analyzes of metabolic properties, iron oxidation and reduction assays are clearly described, and the results are promising.
The research includes a large volume of analyzes and obtained data, which should be utilized in the deeper understanding of the versatile metabolic activities of this microorganism.
A few observations:
pg 4 section 2.8 "aceptores"
2.9 – "Fe3+-citrates, PaFe2N2"
Throughout the text, the symbol for degrees Celsius should be checked
pg 8 – B12 or B12
pg. 9 "between Shewanella sp. T2.3D-1.1 and S. putrefaciens CN-“ – italics
pg 10 - "which may explain why their heavy metal tolerance is than other isolates" - is ?
Author Response
I have activated the "Track changes" option from word so that all changes can be followed easily. All the comments submitted by the reviewers have been addressed and corrected accordingly.
Comment 1: "pg 4 section 2.8 "aceptores"" changed to "acceptors"
Comment 2: "Fe3+-citrates, PaFe2N2" superscipted the "3+" part so that it clearly indicates the redox state of the iron in the molecule.
Comment 3: "Throughout the text, the symbol for degrees Celsius should be checked" 4 symbols have been changed to °C
Comment 4: "pg 8 – B12 or B12" changed to "B12"
Comment 5: "pg. 9 "between Shewanella sp. T2.3D-1.1 and S. putrefaciens CN-“ – italics" changed accordingly in the text
Comment 6: "pg 10 - "which may explain why their heavy metal tolerance is than other isolates" - is ?" I have added "is lowe" and removed the word "acidic". This is because the isolate that we compare to are from marine enviroments and as such non-acidic.
Kindly thank you for your time and comments,
Guillermo Mateos