Vaginal Lactobacillus Impair Candida Dimorphic Switching and Biofilm Formation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Lactobacilli Culture Conditions and Preparation of Planktonic/Biofilm Cell-Free Supernatants
2.2. Candida spp. Culture Conditions and Treatment with Lactobacilli Supernatants
2.3. Evaluation of Candida Dimorphic Switching
2.4. Evaluation of Candida Biofilms
2.4.1. Candida Biofilm Quantification by Crystal Violet Staining
2.4.2. Candida Biofilm Quantification by MTT Assay
2.4.3. Candida Biofilm Visualization by Confocal Microscopy
2.5. Data Analysis
3. Results
3.1. Lactobacilli CFS Impair Candida Dimorphic Switching
3.2. Lactobacilli CFS Impact on Candida Biofilms
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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C. albicans SO1 | C. albicans SO2 | C. lusitaniae SO22 | C. tropicalis SO24 | C. krusei SO26 | C. parapsilosis SO27 | ||
---|---|---|---|---|---|---|---|
L. crispatus | BC1pk | + | + | + | - | + | - |
BC1bf | + | + | + | - | + | + | |
BC3pk | + | - | - | - | - | - | |
BC3bf | + | + | + | - | - | + | |
BC4pk | + | + | - | - | + | - | |
BC4bf | + | + | + | - | + | + | |
BC5pk | + | + | + | - | + | - | |
BC5bf | + | + | + | - | + | + | |
BC6pk | + | + | + | - | + | - | |
BC6bf | + | + | + | - | + | + | |
BC7pk | + | + | - | - | + | - | |
BC7bf | + | + | - | - | + | + | |
BC8pk | + | - | - | - | - | - | |
BC8bf | + | + | + | - | - | + | |
L. gasseri | BC9pk | + | + | + | - | - | + |
BC9bf | - | - | - | - | - | - | |
BC1pk | + | + | + | - | + | - | |
BC1bf | + | + | + | - | + | - | |
BC11pk | + | + | - | - | - | - | |
BC11bf | + | + | - | - | + | - | |
BC12pk | + | + | - | - | - | - | |
BC12bf | + | + | - | - | - | - | |
BC13pk | + | - | + | - | - | - | |
BC13bf | + | - | + | - | - | - | |
BC14pk | + | + | - | - | + | - | |
BC14bf | + | + | - | - | - | - | |
L. vaginalis | BC16pk | - | - | - | - | - | - |
BC16bf | - | - | - | - | - | - | |
BC17pk | - | + | - | - | + | - | |
BC17bf | - | + | - | - | - | - | |
L. plantarum | BC18pk | + | + | - | - | + | - |
BC18bf | + | + | - | - | + | + | |
BC19pk | - | + | - | - | + | - | |
BC19bf | + | + | - | - | - | + |
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Parolin, C.; Croatti, V.; Giordani, B.; Vitali, B. Vaginal Lactobacillus Impair Candida Dimorphic Switching and Biofilm Formation. Microorganisms 2022, 10, 2091. https://doi.org/10.3390/microorganisms10102091
Parolin C, Croatti V, Giordani B, Vitali B. Vaginal Lactobacillus Impair Candida Dimorphic Switching and Biofilm Formation. Microorganisms. 2022; 10(10):2091. https://doi.org/10.3390/microorganisms10102091
Chicago/Turabian StyleParolin, Carola, Vanessa Croatti, Barbara Giordani, and Beatrice Vitali. 2022. "Vaginal Lactobacillus Impair Candida Dimorphic Switching and Biofilm Formation" Microorganisms 10, no. 10: 2091. https://doi.org/10.3390/microorganisms10102091