Whole Genome Sequencing and Comparative Genomics of Indian Isolates of Wheat Spot Blotch Pathogen Bipolaris sorokiniana Reveals Expansion of Pathogenicity Gene Clusters
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection of Isolates
2.2. Establishment of Monoconidial Cultures
2.3. Internal Transcribed Spacer (ITS) Sequencing
2.4. Fungal Pathogenicity Assay by Seed Inoculation Method
2.5. Fungal Pathogenicity Assay by Spray Inoculation Method
2.6. DNA Isolation
2.7. DNA Library Preparation and Genome Sequencing
2.8. Genome Assembly and Assessment
2.8.1. De Novo Assembly
2.8.2. Assessment of Genome Completeness and Phylogenetic Analysis
2.8.3. Genome Synteny
2.8.4. Gene Prediction and Annotation
3. Results
3.1. Cultural Characteristics of the Pathogen Isolates
3.2. Establishment of Monoconidial Cultures
3.3. Pathogen Characterization by ITS Sequencing
3.4. Spore Morphology
3.5. Fungal Pathogenicity Assay by Seed Inoculation Method
3.6. Fungal Pathogenicity Assay by Spray Inoculation Method
3.7. Genome Assembly and Assessment
3.7.1. De Novo Assembly
3.7.2. Assessment of Genome Completeness
3.7.3. Phylogenetic Analysis
3.7.4. Genome Synteny
3.8. Gene Prediction and Annotation
3.9. Gene Orthology
3.10. Prediction of COGs
3.11. Prediction of BGGs
3.12. Prediction of CAZymes
3.13. Prediction of Secretory Proteins
3.14. Prediction of Pathogenicity Genes
3.15. Prediction of Target Genes in D2 Isolate
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate | BS52 | D2 | SI |
---|---|---|---|
Total number of bases | 35,194,358 | 39,322,362 | 32,762,667 |
Number of scaffolds | 280 | 317 | 319 |
Minimum sequence length | 962 | 966 | 893 |
Maximum sequence length | 2,403,846 | 2,565,778 | 2,417,524 |
Average sequence length | 125,246.83 | 123,655.23 | 102,383.33 |
N50 length | 1,054,399 | 803,483 | 1,063,709 |
% (A + T) | 49.31 | 47.19 | 49.16 |
% (G + C) | 48.48 | 50.43 | 49.42 |
Number of genes predicted | 11516 | 15452 | 11351 |
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Yadav, S.; Raazi, Z.; Shivaraj, S.M.; Somani, D.; Prashant, R.; Kulkarni, A.; Kumar, R.; Biradar, S.; Desai, S.; Kadoo, N. Whole Genome Sequencing and Comparative Genomics of Indian Isolates of Wheat Spot Blotch Pathogen Bipolaris sorokiniana Reveals Expansion of Pathogenicity Gene Clusters. Pathogens 2023, 12, 1. https://doi.org/10.3390/pathogens12010001
Yadav S, Raazi Z, Shivaraj SM, Somani D, Prashant R, Kulkarni A, Kumar R, Biradar S, Desai S, Kadoo N. Whole Genome Sequencing and Comparative Genomics of Indian Isolates of Wheat Spot Blotch Pathogen Bipolaris sorokiniana Reveals Expansion of Pathogenicity Gene Clusters. Pathogens. 2023; 12(1):1. https://doi.org/10.3390/pathogens12010001
Chicago/Turabian StyleYadav, Sagar, Zarrine Raazi, Sheelavanta Matha Shivaraj, Deepika Somani, Ramya Prashant, Abhijeet Kulkarni, Rajeev Kumar, Suma Biradar, Shreenivas Desai, and Narendra Kadoo. 2023. "Whole Genome Sequencing and Comparative Genomics of Indian Isolates of Wheat Spot Blotch Pathogen Bipolaris sorokiniana Reveals Expansion of Pathogenicity Gene Clusters" Pathogens 12, no. 1: 1. https://doi.org/10.3390/pathogens12010001