Fatty Acid Signaling Impacts Prostate Cancer Lineage Plasticity in an Autocrine and Paracrine Manner
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Lines
2.3. RNA Preparation, cDNA Synthesis, qPCR
2.4. Immunofluorescence
2.5. Flow Cytometry
2.6. Chromatin Immunoprecipitation (ChIP) Sequensing
2.7. Small-Interfering RNA (siRNA) Transfection
- A: Sense: GCAAGUUGGUACAGGUCAATT,
- antisense: UUGACCUGUACCAACUUGCTT;
- B: Sense: GACAGACCGUCAUAUUCUATT,
- antisense: UAGAAUAUGACGGUCUGUCTT;
- C: Sense: CCAGUGUACUUGAACAGUUTT,
- antisense: AACUGUUCAAGUACACUGGTT.
2.8. Clonogenic Assays
2.9. Animal Studies
2.10. Immunohistochemistry
2.11. Statistical Analysis
3. Results
3.1. Cholesterol and Fatty Acid Signaling in PCa Cells
3.2. Combined Treatment of Palmitate and Cholesterol Induce Lineage Plasticity in PCa Cells by Cancer-Stroma Interactions
3.3. Free Fatty Acid Signaling Mediates PCa Androgen Targeted Therapy Sensitivity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, L.; Billet, S.; Gonzales, G.; Rohena-Rivera, K.; Muranaka, H.; Chu, G.C.-Y.; Yang, Q.; Kim, H.; Bhowmick, N.A.; Smith, B. Fatty Acid Signaling Impacts Prostate Cancer Lineage Plasticity in an Autocrine and Paracrine Manner. Cancers 2022, 14, 3449. https://doi.org/10.3390/cancers14143449
Zhang L, Billet S, Gonzales G, Rohena-Rivera K, Muranaka H, Chu GC-Y, Yang Q, Kim H, Bhowmick NA, Smith B. Fatty Acid Signaling Impacts Prostate Cancer Lineage Plasticity in an Autocrine and Paracrine Manner. Cancers. 2022; 14(14):3449. https://doi.org/10.3390/cancers14143449
Chicago/Turabian StyleZhang, Le, Sandrine Billet, Gabrielle Gonzales, Krizia Rohena-Rivera, Hayato Muranaka, Gina Chia-Yi Chu, Qian Yang, Hyung Kim, Neil A. Bhowmick, and Bethany Smith. 2022. "Fatty Acid Signaling Impacts Prostate Cancer Lineage Plasticity in an Autocrine and Paracrine Manner" Cancers 14, no. 14: 3449. https://doi.org/10.3390/cancers14143449