Temporal Changes in Sparing and Enhancing Dose Protraction Effects of Ionizing Irradiation for Aortic Damage in Wild-Type Mice
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Mice, Irradiation, Shipping and Sampling
2.2. Echocardiography
2.3. FE-SEM
2.4. Immunofluorescence and Histochemistry
2.5. Statistical Analysis
3. Results
3.1. Responses at 12 Months after Starting Irradiation
3.2. Temporal Changes in Biological Effectiveness of Four Irradiation Regimens for Prelesional Aortic Changes
3.2.1. Biological Effectiveness at 6 Months after Starting Irradiation
3.2.2. Biological Effectiveness at 12 Months after Starting Irradiation
3.2.3. Biological Effectiveness at 6 and 12 Months after Starting Irradiation
4. Discussion
4.1. Difference at 6 and 12 Months after Starting Irradiation
4.2. Inter-Regimen Difference in Prelesional Aortic Changes
4.2.1. Enhancing (Inverse) Dose Protraction Effect after X-Rays in 25 Fractions vs. Acute X-Rays
4.2.2. Sparing Dose Protraction Effect after X-Rays in 100 Fractions and Chronic γ-Rays Vis-à-Vis Acute X-Rays
4.2.3. Limitations
4.3. Lesional Changes in the Aorta
4.4. Aortic Changes with Age in Non-Irradiated or Sham-Irradiated Mice
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hamada, N.; Kawano, K.-i.; Nomura, T.; Furukawa, K.; Yusoff, F.M.; Maruhashi, T.; Maeda, M.; Nakashima, A.; Higashi, Y. Temporal Changes in Sparing and Enhancing Dose Protraction Effects of Ionizing Irradiation for Aortic Damage in Wild-Type Mice. Cancers 2022, 14, 3319. https://doi.org/10.3390/cancers14143319
Hamada N, Kawano K-i, Nomura T, Furukawa K, Yusoff FM, Maruhashi T, Maeda M, Nakashima A, Higashi Y. Temporal Changes in Sparing and Enhancing Dose Protraction Effects of Ionizing Irradiation for Aortic Damage in Wild-Type Mice. Cancers. 2022; 14(14):3319. https://doi.org/10.3390/cancers14143319
Chicago/Turabian StyleHamada, Nobuyuki, Ki-ichiro Kawano, Takaharu Nomura, Kyoji Furukawa, Farina Mohamad Yusoff, Tatsuya Maruhashi, Makoto Maeda, Ayumu Nakashima, and Yukihito Higashi. 2022. "Temporal Changes in Sparing and Enhancing Dose Protraction Effects of Ionizing Irradiation for Aortic Damage in Wild-Type Mice" Cancers 14, no. 14: 3319. https://doi.org/10.3390/cancers14143319