New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Setup
2.2. Methods
2.2.1. Force Calibration
2.2.2. How to Calculate the Contact Force?
2.2.3. Position Detection by Particle Tracking
3. Results and Discussion
3.1. Detector Sensitivity Calculation
3.2. Determination of the Force Contact between Two Silica Beads
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Bead Diameter | Laser Power | Depth (µm) | Trap Stiffness kQPD (N/V) | Trap Stiffness kCamera (N/m) | Sensibility Factor β (µm/V) | Final-Trap Stiffness k (N/m) |
---|---|---|---|---|---|---|
0.8 µm | 55.40 | 2.17 | 2.60 × 10−12 | 2.12 × 10−4 | 0.0234 | (4.00 ± 0.02) × 10−3 |
69.05 | 6.58 | 2.50 × 10−12 | 5.80 × 10−4 | 0.0232 | (4.14 ± 0.02) × 10−3 | |
2 µm | 69.05 | 5.25 | 3.38 × 10−12 | 4.47 × 10−5 | 0.1322 | (1.94 ± 0.02) × 10−3 |
58.38 | 5.21 | 3.30 × 10−12 | 4.59 × 10−5 | 0.1386 | (1.74 ± 0.02) × 10−4 |
Bead Diameter | Power (mW) | Trap Stiffness (N/m) | Trap Force (pN) | Contact Force (pN) |
---|---|---|---|---|
2 µm | 55.40 | (1.23 ± 0.01) × 10−4 | 6.330 ± 0.030 | - |
58.38 | (1.74 ± 0.02) × 10−4 | 6.977 ± 0.039 | - | |
69.05 | (1.94 ± 0.02) × 10−4 | 7.180 ± 0.036 | - | |
2 µm–4.5 µm | 55.40 | (5.08 ± 0.01) × 10−5 | 2.529 ± 0.022 | 3.801 ± 0.052 |
58.38 | (5.63 ± 0.01) × 10−5 | 2.533 ± 0.024 | 4.444 ± 0.063 | |
69.05 | (5.77 ± 0.02) × 10−5 | 2.540 ± 0.025 | 4.640 ± 0.061 | |
2 µm–2 µm | 61.94 | (2.75 ± 0.01) × 10−5 | 1.340 ± 0.020 | 4.540 ± 0.060 |
65.49 | (3.33 ± 0.01) × 10−5 | 1.336 ± 0.021 | 4.560 ± 0.058 | |
69.05 | (3.85 ± 0.03) × 10−5 | 1.341 ± 0.018 | 5.840 ± 0.061 |
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Yale, P.; Konin, J.-M.E.; Kouacou, M.A.; Zoueu, J.T. New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer. Micromachines 2018, 9, 425. https://doi.org/10.3390/mi9090425
Yale P, Konin J-ME, Kouacou MA, Zoueu JT. New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer. Micromachines. 2018; 9(9):425. https://doi.org/10.3390/mi9090425
Chicago/Turabian StyleYale, Pavel, Jean-Michel Edoukoua Konin, Michel Abaka Kouacou, and Jérémie Thouakesseh Zoueu. 2018. "New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer" Micromachines 9, no. 9: 425. https://doi.org/10.3390/mi9090425