A Battery of Simple Bioassays for Domestic and Industrial Wastewater Treatment Plants in Konya, Turkey
Round 1
Reviewer 1 Report
Comments and Suggestions for Authors
This work focuses on water treatment systems in domestic and industrial wastewater treatment plants in Konya, Turkey. The article is written in clear and accessible language. The main ideas of this article are beyond doubt. Wastewater treatment systems, their development, construction and other aspects are a relevant topic in our world with a large amount of wastewater. Thus, this work can be considered relevant. There are some points for improvement:
1. The introduction can be expanded by adding references to the literature.
2. It is advisable to compare most of the experimental data obtained with data from literature sources.
3. What patterns are observed in this topic in other regions of Turkey and the World?
4. Discussion can be expanded. So far, the discussion appears modest.
5. Conclusions can be made more concise.
Author Response
"Please see the attachment."
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for Authors
The manuscript is well-designed and well-written. The introduction has the most relevant literature review, however more references are needed to support the discussion.
The methods used to test the toxicity is appropriate and relevant.
The results are clear and well-discussed.
Only minor comments are in the attached manuscript need to be addressed
Comments for author File: Comments.pdf
Author Response
"Please see the attachment."
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for Authors
The reviewed work concerns the assessment of toxicity in domestic and industrial wastewater treatment plants in Konya, Turkey using a battery of tests. The presented research is very interesting. The toxicity of sewage is a very big problem and methods of determining it should be sought. There are many tests that determine the toxicity of sewage, water, soil and sediment. Among these tests, those selected by the authors are also described. The test using Lepidium sativum is often used to determine the toxicity not only of water or sewage but also of soil or sediment. The Vibrio fischeri test is also recommended. In the work, the authors described the novelty of their research and clearly defined the goal. The material and methods are described correctly. They presented the results in figures and tables. There is also a discussion in the work, where the authors present the work of other scientists and compare it with their own research. The summary is clear, correct and understandable.
My comments:
1. In my opinion, Figure 3 is not very legible. Figure 4 can also be changed.
2. Literature - The authors presented 30 sources. The topic of toxicity test batteries has been widely reported. I suggest that the authors cite more new literature.
3. Do the authors have photos of the Lepidium sativum test? I think they can be presented at work.
Author Response
"Please see the attachment."
Author Response File: Author Response.pdf
Reviewer 4 Report
Comments and Suggestions for Authors
REVIEW: A Battery of Simple Bioassay in Domestic and Industrial 3 Wastewater Treatment Plants in Konya, Turkey
ID: sustainability-2765794
The paper presents a theme of great applicability within the WWTP.
1) Introduction: 3 "standard" toxicological tests are reported. Would it be possible to use respirometry to evaluate the toxicity of some substrates?
2) Paragraph 2.1: Would it be possible to insert a graphic indication relating to the sampling positions in the various plants?
3) Paragraph 3.1: The treatment yields of the fundamental parameters for each plant are reported, would it be possible to indicate the variability or a consistency value?
4) For the figures shown in 3D, I would go back to normal 2D viewing. Since there is no need to indicate a variable on the third axis, I think that the two-dimensional figures make the concepts more clear.
Author Response
"Please see the attachment."
Author Response File: Author Response.pdf
