The Diversity and Composition of Soil Microbial Community Differ in Three Typical Wetland Types of the Sanjiang Plain, Northeastern China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Site
2.2. Sampling Method
2.3. Analysis of Physical and Chemical Properties of Soil
2.4. Phospholipid Fatty Acid Analysis (PLFA)
2.5. Data Analysis
- Shannon-Wiener (H): H = ∑(Pi)(lnPi)
- Simpson (D): D = 1 − ΣPi2
- 3.
- Margalef (M): M = (S − 1)/lnN
- 4.
- Menhinick (E): E = S/√N
- 5.
- Brillouin (B): B = N − 1lg(N!/n1!n2!…n!)
3. Results
3.1. Physical and Chemical Properties of the Soil in Different Wetland Types
3.2. Relative Abundance of Soil Microbial PLFA in the Wetlands of the Sanjiang Plain
3.3. Soil Diversity Index Analysis of the Different Wetland Types
3.4. Relationship between Soil Microbial Structure and Soil Chemical Properties
4. Discussion
4.1. Changes in Soil Physicochemical Properties in Different Wetland Types of Sanjiang Plain
4.2. Effects of Wetland Types on Soil Microbial Composition
4.3. Effects of Wetland Types on Soil Microbial Diversity
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wetland Types | Longitude | Latitude | Vegetations |
---|---|---|---|
PF | 133°37′43″ | 47°47′16″ | Calamagrostis Angustifolia, Phragmites australis, Carex miyabei var. maopengensis, Stachys baicalensis, Glyceria spiculosa |
NF | 133°37′51″ | 47°47′21″ | Calamagrostis Angustifolia, Anemone rivularis, Stachys lanata, Sanguisorba tenuifolia, Carex korshinskyi, Lathyrus quinquenervius |
SF | 133°37′04″ | 47°45′39″ | Calamagrostis Angustifolia, Carex korshinskyi, Trollius paluster, Sanguisorba tenuifolia, Geranium linearilobum |
Microbial Group * | PLFA Biomarkers |
---|---|
Unspecified bacteria (BA) | 12:0, 13:0, 14:0, 15:0, 16:0, 17:0, 18:0, 22:0, 24:0 |
Gram-positive bacteria (GP) | a11:0, i13:0, a13:0, i14:0, a14:0, i15:0, a15:0, i15:1ω6c, a16:0, i16:0, a17:0, i17:0, i17:1ω9c, i18:0, i19:0 |
Gram-negative bacteria (GN) | 10:0-2OH, 14:1ω5c, 16:1ω7c, 16:0-2OH, 16:1ω7cDMA, 17:0DMA, 17:1ω8c, 17:0cycloω7c, 19:0cycloω7c, 20:1ω9c, 22:1ω9c |
Actinomycetes (AC) | 10Me17:0, 10Me17:1ω7c, 10Me18:0, 10Me18:1ω7c, 10Me19:1ω7c |
Anaerobic Bacteria (AB) | * 16:2DMA, 18:2DMA |
Aerobic bacteria (AE) | 15:1ω6c |
Desulfobacteria (DE) | 16:0-methyl |
Methanotrophs (ME) | 18:1ω5c, 18:1ω6c, 18:1ω7c |
Unspecified Fungi (FU) | 18:1ω9c, 23:0 |
Arbuscular Mycorrhiza (AM) | 16:1ω5c |
Saprophytic Fungi (SF) | 18:2ω6c |
Protozoa (PR) | 19:3ω6c, 20:3ω6c, 20:4ω6c, 20:5ω3c |
Wetland Type | pH | AN (mg/kg) | AP (mg/kg) | TP (g/kg) | TN (g/kg) | SOC (g/kg) | SWC (%) |
---|---|---|---|---|---|---|---|
Permanently flooded (PF) | 6.00 ± 0.09 a | 496.77 ± 89.02 a | 33.12 ± 1.82 b | 6.36 ± 2.02 b | 4.29 ± 0.08 c | 52.96 ± 2.86 a | 82.67 ± 2.05 a |
Seasonally flooded (SF) | 5.48 ± 0.05 c | 594.4 ± 66.98 a | 30.43 ± 3.61 b | 6.03 ± 1.82 b | 7.62 ± 0.2 a | 35.05 ± 1 c | 52.33 ± 2.05 b |
Non-flooded (NF) | 5.65 ± 0.05 b | 613.56 ± 17.74 a | 43.58 ± 2.67 a | 21.79 ± 1.34 a | 5.62 ± 0.69 b | 46.57 ± 4.46 b | 35.00 ± 2.45 c |
Diversity Index | Permanently Flooded Wetlands | Non-Flooded Wetlands | Seasonally Flooded Wetlands |
---|---|---|---|
Shannon-Wiener (H) | 3.05 ± 0.04 a | 2.12 ± 0.09 b | 3.02 ± 0.07 a |
Simpson (D) | 0.93 ± 0.00 a | 0.71 ± 0.03 b | 0.93 ± 0.01 a |
Margalef (M) | 4.79 ± 0.27 a | 3.38 ± 0.23 b | 4.94 ± 0.11 a |
Menhinick (E) | 0.39 ± 0.02 a | 0.27 ± 0.04 b | 0.42 ± 0.02 a |
Brillouin (B) | 3.02 ± 0.04 a | 2.10 ± 0.09 b | 2.99 ± 0.07 a |
Diversity Index | pH | TP | AP | TN | AN | SOC | SWC |
---|---|---|---|---|---|---|---|
Shannon-Wiener (H) | 0.212 | −0.959 ** | −0.913 ** | 0.072 | −0.432 | −0.118 | 0.864 ** |
Simpson (D) | 0.188 | −0.964 ** | −0.901 ** | 0.080 | −0.427 | −0.107 | 0.855 ** |
Margalef (M) | 0.134 | −0.935 ** | −0.940 ** | 0.157 | −0.320 | −0.228 | 0.791 * |
Menhinick (E) | −0.063 | −0.871 ** | −0.790 * | 0.298 | −0.271 | −0.282 | 0.682 * |
Brillouin (B) | 0.215 | −0.959 ** | −0.914 ** | 0.069 | −0.433 | −0.116 | 0.866 ** |
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Wang, M.; Weng, X.; Zhang, R.; Yang, L.; Liu, Y.; Sui, X. The Diversity and Composition of Soil Microbial Community Differ in Three Typical Wetland Types of the Sanjiang Plain, Northeastern China. Sustainability 2022, 14, 14394. https://doi.org/10.3390/su142114394
Wang M, Weng X, Zhang R, Yang L, Liu Y, Sui X. The Diversity and Composition of Soil Microbial Community Differ in Three Typical Wetland Types of the Sanjiang Plain, Northeastern China. Sustainability. 2022; 14(21):14394. https://doi.org/10.3390/su142114394
Chicago/Turabian StyleWang, Mingyu, Xiaohong Weng, Rongtao Zhang, Libin Yang, Yingnan Liu, and Xin Sui. 2022. "The Diversity and Composition of Soil Microbial Community Differ in Three Typical Wetland Types of the Sanjiang Plain, Northeastern China" Sustainability 14, no. 21: 14394. https://doi.org/10.3390/su142114394