Evaluation of Coal Fly Ash for Modulating the Plant Growth, Yield, and Antioxidant Properties of Daucus carota (L.): A Sustainable Approach to Coal Waste Recycling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Site and Plant Growth Conditions
2.2. Soil and CFA Analysis
2.3. Determination of Growth Biomarkers
2.4. Determination of Leaf Characteristics
2.5. Determination of Biochemical Parameters
2.5.1. Determination of Photosynthetic Pigments
2.5.2. Determination of Nitrate Reductase Activity, Protein, and Carbohydrate Content
2.6. Quantification and Histochemical Localization of Superoxide Anion (O2−)
2.7. Antioxidant Enzyme Assay
2.8. Assessment of Biomineralization in the Edible Part of Carrot
2.9. Statistical Analysis
3. Results
3.1. Effects of CFA Amendment on the Physicochemical Characteristics of the Soil
3.2. Effects of CFA Amended Soil on the Growth and Yield of Carrot
3.3. Effects of CFA Amended Soil on the Leaf Photosynthetic Pigments and Biochemical Markers in the Roots of Carrot
3.4. Effects of CFA Amended soil on the Superoxide Anion (O2•−) in the Leaves of Carrot
3.5. Effect of CFA Amended Soil on the Antioxidant Enzyme Activity in the Edible Part of Carrot
3.6. Effects of CFA Amended Soil on Biomineralization in the Edible Part of Carrot
3.7. Principal Component Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | Soil | CFA | Soil + 15% CFA |
---|---|---|---|
Color | Brownish | Greyish white | Off white |
Texture | Sandy loam | Silty | ---- |
Shape | Flaky | Spherical | Mixed |
pH | 6.97 ± 0.84 | 8.63 ± 0.63 | 7.71 ± 0.56 |
EC (μ mhos cm−1) | 269.44 ± 15.22 | 899.2 ± 30.2 | 449.33 ± 17.2 |
Porosity (%) | 36.87 ± 2.33 | 59.22 ± 3.11 | 43.13 ± 2.23 |
Water holding capacity (%) | 39.67 ± 2.12 | 55.21 ± 2.04 | 45.21 ± 3.41 |
N (%) | 2.22 ± 0.32 | ---- | 1.77 ± 0.23 |
P (g kg−1 soil) | 2.53 ± 0.27 | 0.023 ± 0.002 | 2.21 ± 0.76 |
K (mg L−1) | 21.10 ± 1.23 | 14.1 ± 0.93 | 26.11 ± 1.74 |
Calcium (mg L−1) | 16.32 ± 1.5 | 20.7 ±1.46 | 22.2 ± 2.6 |
Sodium (mg L−1) | 11.6 ± 0.43 | 14.0 ± 0.54 | 13.3 ± 1.6 |
Carbonate (mg L−1) | 75.6 ±3.11 | 67.5 ± 2.87 | 89.3 ± 1.87 |
Bicarbonate (mg L−1) | 16.5 ± 0.96 | 14.5 ± 0.33 | 19.5 ± 0.53 |
Sulphate (mg L−1) | 14.5 ± 0.57 | 22.4 ± 1.23 | 20.55 ± 2.22 |
Chloride (mg L−1) | 23.4 ± 1.22 | 16.5 ± 1.16 | 31.27 ± 2.12 |
Treatments | Shoot Length (cm Plant−1) | Shoot Fresh Weight (g Plant−1) | Shoot Dry Weight (g Plant−1) | Leaves (Plant−1) |
---|---|---|---|---|
C | 83.66± 2.71 bc | 68.76 ± 1.28 c | 18.41 ± 1.21 c | 6.2 ± 0.78 bc |
T1 | 85.87 ± 3.44 bc | 70.35 ± 2.97 b | 18.96 ± 1.89 c | 7.23 ± 0.53 b |
T2 | 88.43 ± 3.63 b | 73.14 ± 0.89 b | 20.60 ± 0.90 b | 9.76 ± 1.10 b |
T3 | 93.23 ± 2.23 a | 77.91 ± 2.65 a | 23.17 ± 1.58 a | 13.23 ± 0.68 a |
T4 | 87.03 ± 2.57 b | 74.66 ± 3.56 b | 20.36 ± 2.56 b | 10.33 ± 0.78 ab |
T5 | 84.09 ± 3.82 bc | 69.34 ± 1.82 bc | 17.95 ± 1.91 c | 8.72 ± 0.62 b |
Treatments | Root Length (cm Plant−1) | Root Circumference (cm Plant−1) | Root Fresh Weight (g Plant−1) | Root Dry Weight (g Plant−1) |
---|---|---|---|---|
C | 15.46 ± 1.26 c | 8.60 ± 1.32 c | 35.52 ± 1.92 c | 8.07 ± 0.91 c |
T1 | 16.98 ± 1.03 c | 8.99 ± 1.49 c | 36.33 ± 2.37 c | 9.90 ± 1.30 bc |
T2 | 19.6 ± 1.44 b | 9.31 ± 1.24 b | 39.66 ± 2.09 b | 11.21 ± 1.23 b |
T3 | 22.1 ± 0.99 a | 11.71 ± 1.29 a | 44.12 ± 1.66 a | 14.30 ± 1.26 a |
T4 | 19.2 ± 1.55 | 10.70 ± 1.42 ab | 40.14 ± 0.98 b | 11.06 ± 1.32 b |
T5 | 16.9 ± 1.45 c | 9.32 ± 1.43 bc | 36.21 ± 1.12 c | 8.12 ± 1.27 c |
Elements in the Edible Part of Carrot | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Treatments | C | N | O | K | Mg | Na | Ca | Fe | Mn | Zn | Si | Cu | Cr | Pb | Cd |
C | 44.16 ± 0.63 | 10.06 ± 0.28 | 44.15 ± 0.55 | 0.69 ± 0.02 | 0.65 ± 0.08 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
T1 | 44.13 ± 0.58 | 8.40 ± 0.32 | 44.45 ± 0.43 | 0.71 ± 0.05 | 0.68 ± 0.26 | 0.34 ± 0.01 | 0.03 ± 0.007 | 0.51 ± 0.014 | 0.27 ± 0.043 | 0.17 ± 0.021 | 0.12 ± 0.011 | ND | ND | ND | ND |
T2 | 42.63 ± 0.75 | 7.10 ± 0.45 | 46.80 ± 0.65 | 0.73 ± 0.06 | 0.91 ± 0.17 | 0.36 ± 0.04 | 0.04 ± 0.004 | 0.58 ± 0.033 | 0.31 ± 0.013 | 0.19 ± 0.015 | 0.14 ± 0.008 | ND | ND | ND | ND |
T3 | 39.67 ± 0.37 | 6.52 ± 0.37 | 49.73 ± 0.43 | 0.77 ± 0.021 | 1.03 ± 0.25 | 0.44 ± 0.07 | 0.06 ± 0.002 | 0.65 ± 0.041 | 0.37 ± 0.021 | 0.22 ± 0.022 | 0.16 ± 0.003 | ND | ND | ND | ND |
T4 | 38.21 ± 0.51 | 5.16 ± 0.18 | 52.12 ± 0.29 | 0.79 ± 0.032 | 1.07 ± 0.21 | 0.46 ± 0.03 | 0.08 ± 0.006 | 0.69 ± 0.033 | 0.39 ± 0.032 | 0.24 ± 0.012 | 0.19 ± 0.005 | 0.21 ± 0.02 | 0.23 ± 0.02 | ND | ND |
T5 | 35.15 ± 0.74 | 3.62 ± 0.43 | 55.88 ± 0.86 | 0.81 ± 0.056 | 1.09 ± 0.43 | 0.48 ± 004 | 0.10 ± 0.002 | 0.72 ± 0.012 | 0.41 ± 0.042 | 0.26 ± 0.002 | 0.22 ± 0.007 | 0.29 ± 0.02 | 0.27 ± 0.01 | 0.17 ± 0.002 | 0.13 ± 0.002 |
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Shakeel, A.; Khan, A.A.; Alharby, H.F.; Bamagoos, A.A.; Tombuloglu, H.; Hakeem, K.R. Evaluation of Coal Fly Ash for Modulating the Plant Growth, Yield, and Antioxidant Properties of Daucus carota (L.): A Sustainable Approach to Coal Waste Recycling. Sustainability 2021, 13, 5116. https://doi.org/10.3390/su13095116
Shakeel A, Khan AA, Alharby HF, Bamagoos AA, Tombuloglu H, Hakeem KR. Evaluation of Coal Fly Ash for Modulating the Plant Growth, Yield, and Antioxidant Properties of Daucus carota (L.): A Sustainable Approach to Coal Waste Recycling. Sustainability. 2021; 13(9):5116. https://doi.org/10.3390/su13095116
Chicago/Turabian StyleShakeel, Adnan, Abrar Ahmad Khan, Hesham F. Alharby, Atif A. Bamagoos, Huseyin Tombuloglu, and Khalid Rehman Hakeem. 2021. "Evaluation of Coal Fly Ash for Modulating the Plant Growth, Yield, and Antioxidant Properties of Daucus carota (L.): A Sustainable Approach to Coal Waste Recycling" Sustainability 13, no. 9: 5116. https://doi.org/10.3390/su13095116