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Hematology Reports
Volume 9
Issue 3
10.4081/hr.2017.7051
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Article

Single Step Multiple Genotyping by MALDI-TOF Mass Spectrometry, for Evaluation of Minor Histocompatibility Antigens in Patients Submitted to Allogeneic Stem Cell Transplantation from HLA-Matched Related and Unrelated Donor

by
Federica Cattina
1,*,
Simona Bernardi
1,
Vilma Mantovani
2,
Eleonora Toffoletti
3,
Alessandra Santoro
4,
Domenico Pastore
5,
Bruno Martino
6,
Giuseppe Console
7,
Giovanni Martinelli
8 and
Michele Malagola
1
1
Unit of Blood Disease and Stem Cell Transplantation, Department of Clinical and Experimental Sciences, University of Brescia, ASST Spedali Civili of Brescia, P.le Ospedali Civili 1, 25123 Brescia, Italy
2
Center for Applied Biomedical Research (CRBA), St. Orsola-Malpighi University Hospital, 40138 Bologna, Italy
3
Division of Hematology and Bone Marrow Transplantation, Department of Experimental and Clinical Medical Sciences, Azienda Ospedaliero-Universitaria di Udine, 33100 Udine, Italy
4
Division of Hematology and Bone Marrow Transplantation, Ospedali Riuniti Villa Sofia-Cervello, 90146 Palermo, Italy
5
Hematology and Bone Marrow Transplantation Unit, Department of Emergency and Organ Transplantation, University of Bari, 70121 Bari, Italy
6
Hematology Division, AO BMM, 89124 Reggio Calabria, Italy
7
Hematology and Bone Marrow Transplant Unit, AO BMM, 89124 Reggio Calabria, Italy
8
Department of Experimental, Diagnostic and Specialty Medicine, Hematology “L.eA. Seràgnoli”, University of Bologna, 40126 Bologna, Italy
*
Author to whom correspondence should be addressed.
Hematol. Rep. 2017, 9(3), 7051; https://doi.org/10.4081/hr.2017.7051
Submission received: 17 January 2017 / Revised: 11 March 2017 / Accepted: 11 April 2017 / Published: 26 September 2017

Abstract

The outcome of patients underwent to allogeneic stem cell transplantation (allo- SCT) is closely related to graft versus host disease (GvHD) and graft versus leukemia (GvL) effects which can be mediated by mHAgs. 23 mHAgs have been identified and reported to be differently correlated with GVHD or GVL and the aim of this work was develop a method to genotype the mHAgs described so far. For this study we used MALDI-TOF iPLEX Gold Mass Array technology. We tested 46 donor/recipient matched pairs that underwent allo-SCT because of Philadelphia positive (Ph+) chronic myeloid leukemia (n = 29) or Ph+ acute lymphoblastic leukemia (n =17). Our data show that sibling pairs had a lesser number of mHAgs mismatches compared to MUD pairs. Notably, donor/recipient genomic mismatch on DPH1 was correlated with an increased risk of acute GvHD and LB-ADIR-1R mismatch on graft versus host direction was correlated with a better RFS with no increase of GvHD risk. Our work provides a simple, accurate and highly automatable method for mHAgs genotyping and suggest the role of mHAgs in addressing the immune reaction between donor and host.
Keywords: stem cell transplantation; HLA; minor histocompatibility antigens stem cell transplantation; HLA; minor histocompatibility antigens

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MDPI and ACS Style

Cattina, F.; Bernardi, S.; Mantovani, V.; Toffoletti, E.; Santoro, A.; Pastore, D.; Martino, B.; Console, G.; Martinelli, G.; Malagola, M. Single Step Multiple Genotyping by MALDI-TOF Mass Spectrometry, for Evaluation of Minor Histocompatibility Antigens in Patients Submitted to Allogeneic Stem Cell Transplantation from HLA-Matched Related and Unrelated Donor. Hematol. Rep. 2017, 9, 7051. https://doi.org/10.4081/hr.2017.7051

AMA Style

Cattina F, Bernardi S, Mantovani V, Toffoletti E, Santoro A, Pastore D, Martino B, Console G, Martinelli G, Malagola M. Single Step Multiple Genotyping by MALDI-TOF Mass Spectrometry, for Evaluation of Minor Histocompatibility Antigens in Patients Submitted to Allogeneic Stem Cell Transplantation from HLA-Matched Related and Unrelated Donor. Hematology Reports. 2017; 9(3):7051. https://doi.org/10.4081/hr.2017.7051

Chicago/Turabian Style

Cattina, Federica, Simona Bernardi, Vilma Mantovani, Eleonora Toffoletti, Alessandra Santoro, Domenico Pastore, Bruno Martino, Giuseppe Console, Giovanni Martinelli, and Michele Malagola. 2017. "Single Step Multiple Genotyping by MALDI-TOF Mass Spectrometry, for Evaluation of Minor Histocompatibility Antigens in Patients Submitted to Allogeneic Stem Cell Transplantation from HLA-Matched Related and Unrelated Donor" Hematology Reports 9, no. 3: 7051. https://doi.org/10.4081/hr.2017.7051

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