Decellularized Lymph Node Scaffolding as a Carrier for Dendritic Cells to Induce Anti-Tumor Immunity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Decellularization of Lymph Nodes
2.2. The Microstructure of dLN Scaffolds
2.3. Biochemical Analysis and Histological Examination
2.4. Recellularization of BMDC into dLN Scaffolds
2.5. Stimulation of BMDC-dLN and the Cytokine Profile
2.6. Immunization of Mice and Tumor Challenge
2.7. Analysis of the Cell Populations inside the BMDC-dLN after Immunization
2.8. Ex Vivo Re-Stimulation of Splenocytes
2.9. Statistical Analysis
3. Results
3.1. The Efficacy of the Decellularization Process
3.2. Microstructure and ECM Examination of the dLN Scaffolds
3.3. Maturation Marker Analysis and Cytokines Released by BMDC-dLN
3.4. Protective Effect of the BMDC-dLN Vaccine against Tumor Challenge
3.5. Analysis of Cell Populations in BMDC-dLN after Immunization
3.6. Ex vivo Re-Stimulation of Splenocytes
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lin, H.-J.; Wang, W.; Huang, Y.-Y.; Liao, W.-T.; Lin, T.-Y.; Lin, S.-Y.; Liu, D.-Z. Decellularized Lymph Node Scaffolding as a Carrier for Dendritic Cells to Induce Anti-Tumor Immunity. Pharmaceutics 2019, 11, 553. https://doi.org/10.3390/pharmaceutics11110553
Lin H-J, Wang W, Huang Y-Y, Liao W-T, Lin T-Y, Lin S-Y, Liu D-Z. Decellularized Lymph Node Scaffolding as a Carrier for Dendritic Cells to Induce Anti-Tumor Immunity. Pharmaceutics. 2019; 11(11):553. https://doi.org/10.3390/pharmaceutics11110553
Chicago/Turabian StyleLin, Hung-Jun, Weu Wang, Yi-You Huang, Wei-Tsen Liao, Ting-Yu Lin, Shyr-Yi Lin, and Der-Zen Liu. 2019. "Decellularized Lymph Node Scaffolding as a Carrier for Dendritic Cells to Induce Anti-Tumor Immunity" Pharmaceutics 11, no. 11: 553. https://doi.org/10.3390/pharmaceutics11110553