Non-Mutated Nucleophosmin 1 Is Recognized by the CD8+ T Lymphocytes of an AML Patient after the Transplantation of Hematopoietic Stem Cells from an HLA-Haploidentical Donor
, Kamila Alexova-Zurkova 1, Petr Hainz 1, Jitka Krystofova 1, Jana Mackova 1, Katerina Roubalova 1, Marketa Stastna-Markova 1,2, Milena Vrana 1 and Jan Vydra 1,2
Round 1
Reviewer 1 Report
The work entitled "Non-mutated nucleophosmin is recognized by CD8+ T lymphocytes of an AML patient after transplantation of hematopoietic stem cells from an HLA-haploidentical donor" is well written, objectively outlining the T cell response specific for both mutated and non- mutated NPM1 elicited in a HSCT AML recipient harboring NPM1c+ mutation.
The results obtained are consistent with the objectives of the manuscript. The conclusion is consistent with the results obtained. I would have liked to have seen some hypothesis of the NPM1wt recognition mechanism. This does not invalidate the results and conclusions.
The manuscript is OK for publication in Current Oncology.
Author Response
Dear reviewer,
Point 1.
I would have liked to have seen some hypothesis of the NPM1wt recognition mechanism.
Answer
We suggested hyptheses on the mechanism of differences in immunogenicity of NPM1wt and NPM1c+ antigens. They are in rows 185-190 of Discussion
Reviewer 2 Report
Sarka N et al presented an article on "non-mutated NPM1 is recognized by CD8+ T lymphocytes of an AML patient after HSCT from an HLA-haploidentical donor.
Major comment
Although it is about a single case, this study shows paradigmatically an intriguing aspect of the role of immune system on eradicating the AML. It is well presented and the subsequent considerations are useful for the readers. I would suggest to implement the discussion trying to add other similar experience and to produce a biological hypothesis of the described phenomenon.
Minor comment
non-mutated: I would use NPM1wt
row 71. I would suggest: "ideal targetable antigen for"
row 175. I would erase the (2011) year . It would be enough the citation reference.
Author Response
Point 1
I would suggest to implement the discussion trying to add other similar experience and to produce a biological hypothesis of the described phenomenon.
Answer
This phenomenon occurs in transplanted patients rarely and to our knowledge was described once by Amir et al. 2011 (citation 13 in introduction). Possible explanation and hypotheses were inserted in Discussion on r. 185-190. Experiments on their validation are under way.
Minor comment
non-mutated: I would use NPM1wt . The idiom non-mutated was replaced by abbreviation 15times.
row 71. I would suggest: "ideal targetable antigen for". This suggestion was accepted.
row 175. I would erase the (2011) year . It would be enough the citation reference. This suggestion was accepted.
Reviewer 3 Report
This is an important report on graft versus leukemia effect in AML patient with mutated NPM1. The topic is is well presented and the problem is adequately introdiced to the reader. It seems scientifically sound and I recommend to accept it. However, I would recommend to be reviewed also from someone who is an expert in tranplant basic imunlogy field.
Author Response
Revision of English spelling through the manuscript was done once more.
