Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis
, Li-yuan Zhai 1, Zeng-hui Shi 1, Jun Zhao 1,* and Duo Liu 2,*
Round 1
Reviewer 1 Report
The article is novel and interesting. the language is very clear and understandable. However, minor changes are required before final publications
1- The following articles should be cited
https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-018-2417-0
moreover, similar work for different plants should be cited
https://www.frontiersin.org/articles/10.3389/fnut.2022.976607/full
https://pubmed.ncbi.nlm.nih.gov/31805322/
additionally,
https://www.sciencedirect.com/science/article/pii/S2666351122000031
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950075/
2- Introduction line 36, names of biological activities details should be stated.
3- Abbreviation list is strongly recommended
4- Future research and study limitation should be suggested .
Best wishes
Author Response
Dear editor and reviewers:
Thank you very much for your helpful comments. Based on your comments, we have revised the manuscript. Followings are itemized answers to the comments.
- The following articles should be cited
https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-018-2417-0
moreover, similar work for different plants should be cited
https://www.frontiersin.org/articles/10.3389/fnut.2022.976607/full
https://pubmed.ncbi.nlm.nih.gov/31805322/ additionally, https://www.sciencedirect.com/science/article/pii/S2666351122000031 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950075/
Answer: We have referred those articles in the text. (references 8, 12, 14, 27, 28)
- Introduction line 36, names of biological activities details should be stated.
Answer: Thank you for your suggestion, We have added the names of biological activities details in the part of introduction. (Line 36)
- Abbreviation list is strongly recommended
Answer: We have added abbreviation list in the text. (Lines 339-342)
- Future research and study limitation should be suggested.
Answer: We have added future research and study limitation in the discussion. (Lines 221-227)
Reviewer 2 Report
1. The title of the MS needs to be paraphrased since it contains confusing phrases, e.g. "active antioxidant" and "structural characterisation."
2. The MS contained a lack of novelty. No new biological targets or biomarkers were tested against the polysaccharides; in fact, it was only a conventional antioxidant assay. There were also no new compounds or derivatives reported from the A. continentalis.
3. the chemical characterisation of the polysaccharides were no convincing
4. The discussion was very brief and did not elaborate on the findings in the current literature
5. The conclusion did not emphasise the significant contribution of this research to the current literature.
Author Response
Dear editor and reviewers:
Thank you very much for your helpful comments. Based on your comments, we have revised the manuscript. Followings are itemized answers to the comments.
Comments and Suggestions for Authors
- The title of the MS needs to be paraphrased since it contains confusing phrases, e.g. "active antioxidant" and "structural characterisation."
Answer: Thank you for your suggestion, we have revised the title.
- The MS contained a lack of novelty. No new biological targets or biomarkers were tested against the polysaccharides; in fact, it was only a conventional antioxidant assay. There were also no new compounds or derivatives reported from the continentalis.
Answer: In this study, authors compared the structure and biological activity of polysaccharides from different parts of A. continentalis, the research determined that the stem of A. Continentalis could be a potential functional food and medicine instead of the root of A. Continentalis, which was mainly a traditional Chinese medicine. So only the basic structural analysis and antioxidant activity in vitro were explored.
- the chemical characterisation of the polysaccharides were no convincing
Answer: The chemical characterisation of the polysaccharides were analyzed by the methods of NMR and Enzymolysis analysis, which could identify structural of differences polysacchrides from A. continentalis.
- The discussion was very brief and did not elaborate on the findings in the current literature.
Answer: Thank you for your suggestion, the analysis and comparison with current literatures are added in the discussion section.
- The conclusion did not emphasise the significant contribution of this research to the current literature.
Answer: we have added the significant contribution of this research in the conclusion. (Lines 334-338)
Reviewer 3 Report
Authors present an interesting study about polysaccharides extracted from different parts (root, stem, and leaf ) of Aralia Continentalis tree. The results, their discussion, and methodological design are presented in the right way. Polysaccharides extraction, purification, and structural analysis presented from the different types of polysaccharides are very well documented. I only recommend improving the methodological information about liquid chromatographic conditions, used in monosaccharides composition, for example, mobile phase composition, discussion about the retention time of sugars, and so on could be good data for readers.
In line 11, the word three must be changed to tree
Author Response
Dear editor and reviewers:
Thank you very much for your helpful comments. Based on your comments, we have revised the manuscript. Followings are itemized answers to the comments.
Comments and Suggestions for Authors
Authors present an interesting study about polysaccharides extracted from different parts (root, stem, and leaf ) of Aralia Continentalis tree. The results, their discussion, and methodological design are presented in the right way. Polysaccharides extraction, purification, and structural analysis presented from the different types of polysaccharides are very well documented. I only recommend improving the methodological information about liquid chromatographic conditions, used in monosaccharides composition, for example, mobile phase composition, discussion about the retention time of sugars, and so on could be good data for readers.
Answer: Thank you for your suggestion, we will improving the methodological information about liquid chromatographic conditions in our further research.
- In line 11, the word three must be changed to tree
Answer: To better understand this, we have revised this sentense in the text. (Line 12)
Round 2
Reviewer 2 Report
ok
Author Response
Dear reviewer:
Thank you very much for your helpful comments. We have finished the extensive English revisions, and revised the manuscript according to your comments. Followings are itemized answers to the comments.
Reviewer 2:
Comments and Suggestions for Authors
Ok
Answer: Thanks again for your suggestions
