A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry
Abstract
:1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1. Generation of the B-SA Surface
3.2. Characterization of the Dried and Rewetted Biotin-Sa Surface via AIR and AFM
3.3. Optimization of Protective Coating and Preparation of Microarrays
3.4. Optimization of Biotin Linker and Streptavidin Concentration
3.5. Target Detection with Biotin-Conjugated Aptamer Arrays
3.6. Aptamer and Protein Mixed Probe Array
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Aptamer Probe | AIR Probe Concentration (µM) | Greatest Thickness Build from Probe Binding Target, Measured by AIR (Å) | LOB (ng/mL) | LOB (nM) | LOD + SE (ng/mL) | LOD (nM) | Reported KD (nM) |
---|---|---|---|---|---|---|---|
Thrombin 29-mer | 10 | 1.4 | 8.8 | 0.24 | 10.9 ± 271.5 | 0.30 | 0.5 |
Thrombin 29-mer | 50 | 2.1 | 7.6 | 0.21 | 15.3 ± 0.1 | 0.43 | 0.5 |
Thrombin 29-mer | 100 | 1.9 | 5.6 | 0.16 | 12.7 ± 0.1 | 0.35 | 0.5 |
Thrombin 15-mer | 10, 50, 100 | N/A | N/A | N/A | N/A | N/A | 25–200 |
TGFβ1 | 10 | 9.2 | 53.7 | 2.1 | 165.3 ± 0.1 | 6.6 | 90 |
TGFβ1 | 50 | 10.5 | 62.1 | 2.5 | 135.3 ± 0.1 | 5.4 | 90 |
TGFβ1 | 100 | 10.5 | 32.2 | 1.3 | 74.5 ± 0.1 | 3.0 | 90 |
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Klose, A.M.; Miller, B.L. A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry. Sensors 2020, 20, 5745. https://doi.org/10.3390/s20205745
Klose AM, Miller BL. A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry. Sensors. 2020; 20(20):5745. https://doi.org/10.3390/s20205745
Chicago/Turabian StyleKlose, Alanna M., and Benjamin L. Miller. 2020. "A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry" Sensors 20, no. 20: 5745. https://doi.org/10.3390/s20205745