Glycolysis-Stimulated Esrrb Lactylation Promotes the Self-Renewal and Extraembryonic Endoderm Stem Cell Differentiation of Embryonic Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Ldh Inhibition Compromises the Self-Renewal of ESCs
2.2. Esrrb Is Lactylated on K228 and K232
2.3. Esrrb KQ Mutant Is More Potent in Pluripotency Maintenance
2.4. Esrrb Lactylation Promotes XEN Differentiation
2.5. Lactate Regulates XEN Differentiation Mainly by Lactylating Esrrb
2.6. Lactylation of Esrrb Enhances Its Binding to XEN Genes
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Transfection
4.2. Plasmid Construction
4.3. Quantitative Reverse-Transcription PCR (qRT-PCR)
4.4. Colony-Forming Assay and Alkaline Phosphatase (AP) Staining
4.5. Western Blot
4.6. Immunoprecipitation
4.7. Chromatin Immunoprecipitation-Seq (ChIP–Seq)
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dong, Q.; Zhang, Q.; Yang, X.; Nai, S.; Du, X.; Chen, L. Glycolysis-Stimulated Esrrb Lactylation Promotes the Self-Renewal and Extraembryonic Endoderm Stem Cell Differentiation of Embryonic Stem Cells. Int. J. Mol. Sci. 2024, 25, 2692. https://doi.org/10.3390/ijms25052692
Dong Q, Zhang Q, Yang X, Nai S, Du X, Chen L. Glycolysis-Stimulated Esrrb Lactylation Promotes the Self-Renewal and Extraembryonic Endoderm Stem Cell Differentiation of Embryonic Stem Cells. International Journal of Molecular Sciences. 2024; 25(5):2692. https://doi.org/10.3390/ijms25052692
Chicago/Turabian StyleDong, Qiman, Qingye Zhang, Xiaoqiong Yang, Shanshan Nai, Xiaoling Du, and Lingyi Chen. 2024. "Glycolysis-Stimulated Esrrb Lactylation Promotes the Self-Renewal and Extraembryonic Endoderm Stem Cell Differentiation of Embryonic Stem Cells" International Journal of Molecular Sciences 25, no. 5: 2692. https://doi.org/10.3390/ijms25052692