The Effects of Aging on Sarcoplasmic Reticulum-Related Factors in the Skeletal Muscle of Mice
Abstract
:1. Introduction
2. Results
2.1. Body Weight, Muscle Weight, and Relative Muscle Weight
2.2. SERCA-Related Factors
2.3. SERCA Localization
2.4. Histochemical Findings
2.5. Electron Microscopic Analysis
2.6. DHPRα1 and RyR1 Localization
2.7. JPH1 and JPH2 Localization
2.8. Matrix Metalloproteinase 2 Localization
2.9. Ratio of Type I and II Fibers
2.10. DHPRα1 and SERCA1 Co-Localization
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Sampling
4.3. qPCR
4.4. IHC
4.5. Morphological Analysis
4.6. Histochemical Analysis
4.7. TEM
4.8. mATPase Staining
4.9. Immunofluorescence
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kanazawa, Y.; Takahashi, T.; Nagano, M.; Koinuma, S.; Shigeyoshi, Y. The Effects of Aging on Sarcoplasmic Reticulum-Related Factors in the Skeletal Muscle of Mice. Int. J. Mol. Sci. 2024, 25, 2148. https://doi.org/10.3390/ijms25042148
Kanazawa Y, Takahashi T, Nagano M, Koinuma S, Shigeyoshi Y. The Effects of Aging on Sarcoplasmic Reticulum-Related Factors in the Skeletal Muscle of Mice. International Journal of Molecular Sciences. 2024; 25(4):2148. https://doi.org/10.3390/ijms25042148
Chicago/Turabian StyleKanazawa, Yuji, Tatsuo Takahashi, Mamoru Nagano, Satoshi Koinuma, and Yasufumi Shigeyoshi. 2024. "The Effects of Aging on Sarcoplasmic Reticulum-Related Factors in the Skeletal Muscle of Mice" International Journal of Molecular Sciences 25, no. 4: 2148. https://doi.org/10.3390/ijms25042148