Differential Immunogenicity and Lung Disease-Inducing Potential of Mycobacterium immunogenum Genotypes and Impact of Co-Exposure with Pseudomonas: Optimizing a Mouse Model of Chronic Hypersensitivity Pneumonitis
Abstract
:1. Introduction
2. Results
2.1. Optimization of Antigen Dose
2.2. Optimization of Timepoint for Sacrifice after the Final Dose Instillation
2.3. Dose–Response Relationship Using Live Cells of MI 700506
2.4. Comparative Immunopathological Responses to Different Genotypes of M. immunogenum
2.5. Co-Exposure with MI 700506 and P. fluorescens
3. Discussion
3.1. Dose–Response Relationship and Optimal Dose Selection
3.2. Capturing the HP-Specific Response Post-Exposure
3.3. Effect of Whole Cells vs. Cell Lysates in Mycobacterial Induction of HP
3.4. Effect of Co-Exposure on Induction of Mycobacterial HP
4. Materials and Methods
4.1. Animals
4.2. Bacterial Strains/Genotypes and Culture Conditions
4.3. Preparation of M. immunogenum (MI) Cell Lysate for Animal Dosing
4.4. Preparation of Saccharopolyspora rectivirgula (SR) Lysate
4.5. Preparation of Monocellular Mycobacterial Suspensions
4.6. Animal Exposure Protocol
4.7. Bronchoalveolar Lavage and Analyses
4.8. Histopathological Analyses
4.9. Splenocyte T-Cell Response Analysis
4.10. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Johansson, E.; Yadav, J.S. Differential Immunogenicity and Lung Disease-Inducing Potential of Mycobacterium immunogenum Genotypes and Impact of Co-Exposure with Pseudomonas: Optimizing a Mouse Model of Chronic Hypersensitivity Pneumonitis. Int. J. Mol. Sci. 2024, 25, 2058. https://doi.org/10.3390/ijms25042058
Johansson E, Yadav JS. Differential Immunogenicity and Lung Disease-Inducing Potential of Mycobacterium immunogenum Genotypes and Impact of Co-Exposure with Pseudomonas: Optimizing a Mouse Model of Chronic Hypersensitivity Pneumonitis. International Journal of Molecular Sciences. 2024; 25(4):2058. https://doi.org/10.3390/ijms25042058
Chicago/Turabian StyleJohansson, Elisabet, and Jagjit S. Yadav. 2024. "Differential Immunogenicity and Lung Disease-Inducing Potential of Mycobacterium immunogenum Genotypes and Impact of Co-Exposure with Pseudomonas: Optimizing a Mouse Model of Chronic Hypersensitivity Pneumonitis" International Journal of Molecular Sciences 25, no. 4: 2058. https://doi.org/10.3390/ijms25042058