Suppression of Neuroinflammation by Coffee Component Pyrocatechol via Inhibition of NF-κB in Microglia
Abstract
:1. Introduction
2. Results
2.1. Coffee and Decaffeinated Coffee Both Inhibited LPS-Induced Inflammatory Responses in BV-2 Cells
2.2. Coffee and Decaffeinated Coffee Both Inhibited the LPS-Induced Nuclear Translocation and Transcriptional Activation of NF-κB in BV-2 Cells
2.3. The Coffee Ingredient, Pyrocatechol Inhibited LPS-Induced Inflammatory Responses in BV-2 Cells
2.4. The Coffee Ingredient, Pyrocatechol, Inhibited the LPS-Induced Nuclear Translocation and Transcriptional Activation of NF-κB in BV-2 Cells
2.5. Coffee and Decaffeinated Coffee Both Attenuated the LPS-Induced Accumulation of Microglia and mRNA Expression of IL-6, TNFα, CCL2, and CXCL1 in Mice
2.6. The Intake of Pyrocatechol Attenuated the LPS-Induced Accumulation of Microglia and mRNA Expression of IL-6, TNFα, CCL2, and CXCL1 in Mice
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. Cell Culture
4.3. Measurement of Cell Viability and NO Production
4.4. SDS-Polyacrylamide Gel Electrophoresis (PAGE) and Immunoblotting
4.5. Reverse Transcription–Polymerase Chain Reaction (RT-PCR)
4.6. Transfection and Luciferase Assays
4.7. Immunofluorescence Staining
4.8. Induction of Neuroinflammation in Mice Administered LPS
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Murata, T.; Tago, K.; Miyata, K.; Moriwaki, Y.; Misawa, H.; Kobata, K.; Nakazawa, Y.; Tamura, H.; Funakoshi-Tago, M. Suppression of Neuroinflammation by Coffee Component Pyrocatechol via Inhibition of NF-κB in Microglia. Int. J. Mol. Sci. 2024, 25, 316. https://doi.org/10.3390/ijms25010316
Murata T, Tago K, Miyata K, Moriwaki Y, Misawa H, Kobata K, Nakazawa Y, Tamura H, Funakoshi-Tago M. Suppression of Neuroinflammation by Coffee Component Pyrocatechol via Inhibition of NF-κB in Microglia. International Journal of Molecular Sciences. 2024; 25(1):316. https://doi.org/10.3390/ijms25010316
Chicago/Turabian StyleMurata, Taisuke, Kenji Tago, Kota Miyata, Yasuhiro Moriwaki, Hidemi Misawa, Kenji Kobata, Yosuke Nakazawa, Hiroomi Tamura, and Megumi Funakoshi-Tago. 2024. "Suppression of Neuroinflammation by Coffee Component Pyrocatechol via Inhibition of NF-κB in Microglia" International Journal of Molecular Sciences 25, no. 1: 316. https://doi.org/10.3390/ijms25010316