Effective Multivalent Oriented Presentation of Meningococcal NadA Antigen Trimers by Self-Assembling Ferritin Nanoparticles
Abstract
:1. Introduction
2. Results
2.1. Design of Ferritin Nanoparticles Constructs Presenting Trimeric NadA
2.2. NadA-Ferritin Nanoparticles Form Large and Stable Structures in Solution
2.3. NadA-Ferritin Nanoparticles Are Octahedral Structures
2.4. NadA_Ext-Fe Nanoparticles Preserve the Functional Epitopes of NadA
2.5. NadA-Ferritin Nanoparticles Potently Induce Complement-Mediated Bactericidal Activity and Associated IgG Subclasses
3. Discussion
4. Materials and Methods
4.1. Structure-Based Design
4.2. Cloning and Expression
4.3. Protein Purification
4.4. Cryo-Electron Microscopy (Cryo-EM) Sample Preparation
4.5. Cryo-EM Data Acquisition
4.6. Cryo-EM Data Processing
4.7. Surface Plasmon Resonance (SPR)
4.8. Differential Scanning Fluorimetry (DSF)
4.9. Differential Scanning Calorimetry (DSC)
4.10. Mice Immunizations
4.11. Complement-Mediated Bactericidal Activity
4.12. ELISA
4.13. Statistical Analysis of ELISA Data
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Veggi, D.; Dello Iacono, L.; Malito, E.; Maruggi, G.; Giusti, F.; Goswami, P.; Pansegrau, W.; Marchi, S.; Tomei, S.; Luzzi, E.; et al. Effective Multivalent Oriented Presentation of Meningococcal NadA Antigen Trimers by Self-Assembling Ferritin Nanoparticles. Int. J. Mol. Sci. 2023, 24, 6183. https://doi.org/10.3390/ijms24076183
Veggi D, Dello Iacono L, Malito E, Maruggi G, Giusti F, Goswami P, Pansegrau W, Marchi S, Tomei S, Luzzi E, et al. Effective Multivalent Oriented Presentation of Meningococcal NadA Antigen Trimers by Self-Assembling Ferritin Nanoparticles. International Journal of Molecular Sciences. 2023; 24(7):6183. https://doi.org/10.3390/ijms24076183
Chicago/Turabian StyleVeggi, Daniele, Lucia Dello Iacono, Enrico Malito, Giulietta Maruggi, Fabiola Giusti, Panchali Goswami, Werner Pansegrau, Sara Marchi, Sara Tomei, Enrico Luzzi, and et al. 2023. "Effective Multivalent Oriented Presentation of Meningococcal NadA Antigen Trimers by Self-Assembling Ferritin Nanoparticles" International Journal of Molecular Sciences 24, no. 7: 6183. https://doi.org/10.3390/ijms24076183