Neurog1-Derived Peptides RMNE1 and DualPep-Shine Penetrate the Skin and Inhibit Melanin Synthesis by Regulating MITF Transcription
Abstract
:1. Introduction
2. Results
2.1. Using CPPs for Melanin Inhibition Screening in B16F10 Cells
2.2. RMNE1 and Its Derivatives Inhibit Melanin Synthesis by Suppressing Melanogenesis-Related Enzymes
2.3. DualPep-Shine Regulates the Expression of Melanogenic Genes and Inhibits the Promoter Activity of MITF-M
2.4. Inhibitory Effect of DualPep-Shine on Melanin Synthesis in Neoderm-ME Human Skin
2.5. DualPep-Shine Shows High Stability with Little Toxicity
3. Discussion
4. Materials and Methods
4.1. Cell Viability Assay
4.2. Peptide Synthesis
4.3. Western Blot Analysis
4.4. qRT-PCR
4.5. Melanin Measurement
4.6. Luciferase Reporter Assay
4.7. Measurement of Whitening Effect Using a 3D Human Skin Model, Neoderm-ME
4.8. Penetration Assay
4.9. Franz Diffusion Cells Method
4.10. Statistics
5. Patent
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Song, E.C.; Park, C.; Shin, Y.; Kim, W.K.; Kim, S.B.; Cho, S. Neurog1-Derived Peptides RMNE1 and DualPep-Shine Penetrate the Skin and Inhibit Melanin Synthesis by Regulating MITF Transcription. Int. J. Mol. Sci. 2023, 24, 6158. https://doi.org/10.3390/ijms24076158
Song EC, Park C, Shin Y, Kim WK, Kim SB, Cho S. Neurog1-Derived Peptides RMNE1 and DualPep-Shine Penetrate the Skin and Inhibit Melanin Synthesis by Regulating MITF Transcription. International Journal of Molecular Sciences. 2023; 24(7):6158. https://doi.org/10.3390/ijms24076158
Chicago/Turabian StyleSong, Ee Chan, Chanho Park, Yungyeong Shin, Wan Ki Kim, Sang Bum Kim, and Seongmin Cho. 2023. "Neurog1-Derived Peptides RMNE1 and DualPep-Shine Penetrate the Skin and Inhibit Melanin Synthesis by Regulating MITF Transcription" International Journal of Molecular Sciences 24, no. 7: 6158. https://doi.org/10.3390/ijms24076158