Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. Effect of Allicin on Cell Viability and Inflammatory Cytokine Levels
2.2. Effect of Allicin on LPS-Induced Activation of the NLRP3 Inflammasome
2.3. Effect of Allicin on TLR4 Expression
2.4. Effect of Allicin on the NF-κB Signaling Pathway
2.5. Effect of Allicin on LPS-Induced Histopathological Changes
2.6. Effect of Allicin on the Level of Inflammation in an LPS-Induced Model of Mastitis In Vitro
3. Discussion
4. Materials and Methods
4.1. Animal Handling and Experimental Groups
4.2. Cell Culture and Processing
4.3. Cell Viability Assay
4.4. RNA Extraction and RT–qPCR
4.5. Western Blotting
4.6. Histopathological Analysis
4.7. MPO Activity Determination
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Che, H.-Y.; Zhou, C.-H.; Lyu, C.-C.; Meng, Y.; He, Y.-T.; Wang, H.-Q.; Wu, H.-Y.; Zhang, J.-B.; Yuan, B. Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells. Int. J. Mol. Sci. 2023, 24, 3805. https://doi.org/10.3390/ijms24043805
Che H-Y, Zhou C-H, Lyu C-C, Meng Y, He Y-T, Wang H-Q, Wu H-Y, Zhang J-B, Yuan B. Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells. International Journal of Molecular Sciences. 2023; 24(4):3805. https://doi.org/10.3390/ijms24043805
Chicago/Turabian StyleChe, Hao-Yu, Chang-Hai Zhou, Chen-Chen Lyu, Yu Meng, Yun-Tong He, Hao-Qi Wang, Hong-Yu Wu, Jia-Bao Zhang, and Bao Yuan. 2023. "Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells" International Journal of Molecular Sciences 24, no. 4: 3805. https://doi.org/10.3390/ijms24043805