Fever-Range Hyperthermia Promotes Macrophage Polarization towards Regulatory Phenotype M2b
Abstract
:1. Introduction
2. Results
2.1. Fever-Range Hyperthermia Induces Expression of M2-like Surface Markers
2.2. Fever-Range Hyperthermia Modifies LPS-Induced M1 Polarization towards M2 Phenotype
2.3. FRH-Induced M2-like Cells Express Pro-Inflammatory Proteins but Reveal Anti-Inflammatory Properties
2.4. Fever-Range Hyperthermia Changes Macrophage Phenotype in a TLR-4-Independent Way?
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Measurement of Intracellular Reactive Oxygen Species (ROS)
4.3. Nitric Oxide Determination
4.4. Western Blot Analysis
4.5. Analysis of Macrophage Polarization by Flow Cytometry
4.6. Analysis of Cytokine Expression by Real-Time PCR
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primary Antibodies | ||||
---|---|---|---|---|
Protein Name | Protein Symbol | Cat. No. | Source/Isotype | Company |
Cyclooxygenase 2 | COX-2 | #12282 | Rabbit IgG | Cell Signaling Technology (Danvers, MA, USA) |
Inducible Nitric Oxide Synthase | iNOS | #2982 | Rabbit IgG | Cell Signaling Technology |
Toll-like Receptor 4 | TLR-4 | #14358 | Rabbit IgG | Cell Signaling Technology |
Arginase-1 | Arg-1 | #93668 | Rabbit IgG | Cell Signaling Technology |
Actin | Actb | 612657 | Mouse IgG | BD Bioscience (Franklin Lakes, NJ, USA) |
Secondary Antibodies | ||||
Target | Origin | Type of conjugate | Company | |
Anti-Rabbit | Goat IgG | Peroxidase-conjugated Anti-Rabbit | Sigma Aldrich | |
Anti-Mouse | Goat IgG | Peroxidase-conjugated Anti-Mouse | Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA) |
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Kozłowski, H.M.; Sobocińska, J.; Jędrzejewski, T.; Maciejewski, B.; Dzialuk, A.; Wrotek, S. Fever-Range Hyperthermia Promotes Macrophage Polarization towards Regulatory Phenotype M2b. Int. J. Mol. Sci. 2023, 24, 17574. https://doi.org/10.3390/ijms242417574
Kozłowski HM, Sobocińska J, Jędrzejewski T, Maciejewski B, Dzialuk A, Wrotek S. Fever-Range Hyperthermia Promotes Macrophage Polarization towards Regulatory Phenotype M2b. International Journal of Molecular Sciences. 2023; 24(24):17574. https://doi.org/10.3390/ijms242417574
Chicago/Turabian StyleKozłowski, Henryk Mikołaj, Justyna Sobocińska, Tomasz Jędrzejewski, Bartosz Maciejewski, Artur Dzialuk, and Sylwia Wrotek. 2023. "Fever-Range Hyperthermia Promotes Macrophage Polarization towards Regulatory Phenotype M2b" International Journal of Molecular Sciences 24, no. 24: 17574. https://doi.org/10.3390/ijms242417574