Maize Dek407 Encodes the Nitrate Transporter 1.5 and Is Required for Kernel Development
Abstract
:1. Introduction
2. Results
2.1. Phenotypic and Genetic Analysis of the dek407 Mutant
2.2. Positional Cloning and Identification of the Dek407 Gene
2.3. Dek407 Encodes a Highly Conserved Nitrate Transporter Protein and Is Predominantly Expressed in Developing Seeds
2.4. Comparative Transcriptome Analysis of Developing Kernels of the dek407 Mutant and WT
2.5. Mutation of Dek407 Alters the Expression of Genes Associated with Phytohormones
2.6. The Phytohormone Balance Was Altered in the dek407 Mutant
2.7. Natural Variations in Dek407 Are Associated with Kernel Weight and Kernel Size
3. Discussion
3.1. The Function of DEK407 Differs from Its Homologs in Rice and Arabidopsis
3.2. Reduction in Auxin in the dek407 Mutant May Be a New Cause for the Defective Kernel Phenotype
3.3. The Amino Acid Residues in Different TMs May Confer Their Specificity for Various Substrate Transportation
3.4. Dek407 Is a Potential Target for the Improvement of Maize Yield
4. Materials and Methods
4.1. Plant Materials
4.2. Light Microscopy of Cytological Sections
4.3. Measurement of Hundred-Kernel Weight and Starch Content
4.4. Bulked Segregant RNA-Seq Analysis
4.5. Map-Based Cloning of Dek407
4.6. Sequence BLAST and Phylogenetic Tree Construction
4.7. RNA-Sequencing Analysis
4.8. Measurement of Endogenous Phytohormones
4.9. Candidate Gene-Based Association Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, H.; Yan, X.; Du, Q.; Yan, P.; Xi, J.; Meng, X.; Li, X.; Liu, H.; Liu, G.; Fu, Z.; et al. Maize Dek407 Encodes the Nitrate Transporter 1.5 and Is Required for Kernel Development. Int. J. Mol. Sci. 2023, 24, 17471. https://doi.org/10.3390/ijms242417471
Wang H, Yan X, Du Q, Yan P, Xi J, Meng X, Li X, Liu H, Liu G, Fu Z, et al. Maize Dek407 Encodes the Nitrate Transporter 1.5 and Is Required for Kernel Development. International Journal of Molecular Sciences. 2023; 24(24):17471. https://doi.org/10.3390/ijms242417471
Chicago/Turabian StyleWang, Hongqiu, Xiaolan Yan, Qingguo Du, Pengshuai Yan, Jinjin Xi, Xiaoruo Meng, Xuguang Li, Huijian Liu, Guoqin Liu, Zhongjun Fu, and et al. 2023. "Maize Dek407 Encodes the Nitrate Transporter 1.5 and Is Required for Kernel Development" International Journal of Molecular Sciences 24, no. 24: 17471. https://doi.org/10.3390/ijms242417471