Surface Modification of Polylactic Acid Bioscaffold Fabricated via 3D Printing for Craniofacial Bone Tissue Engineering
Abstract
:1. Introduction
2. Results
2.1. Three-Dimensionally Printed PLA Scaffold
2.2. Surface Modification of PLA Scaffolds by Hydrogel
2.3. BMSCs Viability in PLA Scaffolds
2.4. Osteogenic Potential of BMSCs Cultured in PLA Scaffolds
2.5. In Vivo Bone Regeneration Study
3. Discussion
4. Materials and Methods
4.1. BMSCs Extraction and Expansion from Rats
4.2. PLA Scaffold Preparation
4.3. In Vitro Test
4.3.1. Cell Cultures
4.3.2. Scaffold Degradation Test
4.3.3. SEM Observation
4.3.4. Live and Dead Test
4.3.5. Real-Time PCR
4.4. In Vivo Study of Bone Regeneration
4.4.1. In Vivo Animal Study
4.4.2. Micro-CT
4.4.3. Histological Examinations
4.5. Statistical Evaluation
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, Y.-C.; Lo, G.-J.; Shyu, V.B.-H.; Tsai, C.-H.; Chen, C.-H.; Chen, C.-T. Surface Modification of Polylactic Acid Bioscaffold Fabricated via 3D Printing for Craniofacial Bone Tissue Engineering. Int. J. Mol. Sci. 2023, 24, 17410. https://doi.org/10.3390/ijms242417410
Liu Y-C, Lo G-J, Shyu VB-H, Tsai C-H, Chen C-H, Chen C-T. Surface Modification of Polylactic Acid Bioscaffold Fabricated via 3D Printing for Craniofacial Bone Tissue Engineering. International Journal of Molecular Sciences. 2023; 24(24):17410. https://doi.org/10.3390/ijms242417410
Chicago/Turabian StyleLiu, Yao-Chang, Guan-Jie Lo, Victor Bong-Hang Shyu, Chia-Hsuan Tsai, Chih-Hao Chen, and Chien-Tzung Chen. 2023. "Surface Modification of Polylactic Acid Bioscaffold Fabricated via 3D Printing for Craniofacial Bone Tissue Engineering" International Journal of Molecular Sciences 24, no. 24: 17410. https://doi.org/10.3390/ijms242417410