The Effect of Mice Adaptation Process on the Pathogenicity of Influenza A/South Africa/3626/2013 (H1N1)pdm09 Model Strain
Abstract
:1. Introduction
2. Results
2.1. Sequencing Analysis of SA-M5
2.2. Viral Replication in Lung Tissue
2.3. SA-WT and SA-M5 Toxicity in Mice
2.4. SA-WT and SA-M5 Pathogenicity in Mice
2.5. SA-WT and SA-M5 Immunogenicity
3. Discussion
4. Materials and Methods
4.1. Viruses
4.2. Mice
4.3. Virus Adaptation to Mice
4.4. Viral Replication in Lung Tissue
4.5. Viral Genome Sequencing
4.6. Toxicity in Mice
4.7. Pathogenicity in Mice
4.8. Hemagglutination Inhibition Test
4.9. 3D Structure
4.10. Ethics Statement
4.11. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Al Farroukh, M.; Kiseleva, I.; Stepanova, E.; Bazhenova, E.; Krutikova, E.; Tkachev, A.; Chistyakova, A.; Rekstin, A.; Puchkova, L.; Rudenko, L. The Effect of Mice Adaptation Process on the Pathogenicity of Influenza A/South Africa/3626/2013 (H1N1)pdm09 Model Strain. Int. J. Mol. Sci. 2023, 24, 17386. https://doi.org/10.3390/ijms242417386
Al Farroukh M, Kiseleva I, Stepanova E, Bazhenova E, Krutikova E, Tkachev A, Chistyakova A, Rekstin A, Puchkova L, Rudenko L. The Effect of Mice Adaptation Process on the Pathogenicity of Influenza A/South Africa/3626/2013 (H1N1)pdm09 Model Strain. International Journal of Molecular Sciences. 2023; 24(24):17386. https://doi.org/10.3390/ijms242417386
Chicago/Turabian StyleAl Farroukh, Mohammad, Irina Kiseleva, Ekaterina Stepanova, Ekaterina Bazhenova, Elena Krutikova, Artem Tkachev, Anna Chistyakova, Andrey Rekstin, Ludmila Puchkova, and Larisa Rudenko. 2023. "The Effect of Mice Adaptation Process on the Pathogenicity of Influenza A/South Africa/3626/2013 (H1N1)pdm09 Model Strain" International Journal of Molecular Sciences 24, no. 24: 17386. https://doi.org/10.3390/ijms242417386