Culture of Human Rotaviruses in Relevant Models Shows Differences in Culture-Adapted and Nonculture-Adapted Strains
Abstract
:1. Introduction
2. Results
2.1. RV from Clinical Samples Infect Diferentiated Caco-2 Cells
2.2. Viral Replication in Relevant Cell Culture Models Expressing HBGAs
2.3. The VP8* Protein from V7 RV Recognizes the H1 Antigen with Better Avidity Compared to Wa
3. Discussion
4. Materials and Methods
4.1. Human Rotavirus Stool Filtrate Preparation
4.2. Cell Culture Maintenance
4.3. Infection of Cell Lines with Human Rotavirus
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- Infection of two 96-well plates with dCaco-2 (timepoints of 1 hpi and 72 hpi) to screen for viral infectivity of collected viral filtrates. Those with the ability to infect were used in the following experiments;
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- Infection of five 96-well plates with dCaco-2 cells (timepoints of 1, 24, 48, 72, and 96 hpi) To determine the peak of infection for selected filtrates;
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- Infection of two 96-well plates with dCaco-2 cells, uCaco-2 cells, MA104, HT29, and HT29-M6 (timepoints of 1 hpi and 72 hpi) to compare the ability of the virus to infect each cell type.
4.4. Human Intestinal Enteroid (HIE) Culture and Monolayer Preparation
4.5. HIE Infection with RV and Stool Filtrates
4.6. RNA Extraction, RT-qPCR and 2−ΔΔCt
4.7. Immunofluorescence
4.8. VP8* Production and Interaction Assay
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Peña-Gil, N.; Randazzo, W.; Carmona-Vicente, N.; Santiso-Bellón, C.; Cárcamo-Cálvo, R.; Navarro-Lleó, N.; Monedero, V.; Yebra, M.J.; Buesa, J.; Gozalbo-Rovira, R.; et al. Culture of Human Rotaviruses in Relevant Models Shows Differences in Culture-Adapted and Nonculture-Adapted Strains. Int. J. Mol. Sci. 2023, 24, 17362. https://doi.org/10.3390/ijms242417362
Peña-Gil N, Randazzo W, Carmona-Vicente N, Santiso-Bellón C, Cárcamo-Cálvo R, Navarro-Lleó N, Monedero V, Yebra MJ, Buesa J, Gozalbo-Rovira R, et al. Culture of Human Rotaviruses in Relevant Models Shows Differences in Culture-Adapted and Nonculture-Adapted Strains. International Journal of Molecular Sciences. 2023; 24(24):17362. https://doi.org/10.3390/ijms242417362
Chicago/Turabian StylePeña-Gil, Nazaret, Walter Randazzo, Noelia Carmona-Vicente, Cristina Santiso-Bellón, Roberto Cárcamo-Cálvo, Noemi Navarro-Lleó, Vicente Monedero, María J. Yebra, Javier Buesa, Roberto Gozalbo-Rovira, and et al. 2023. "Culture of Human Rotaviruses in Relevant Models Shows Differences in Culture-Adapted and Nonculture-Adapted Strains" International Journal of Molecular Sciences 24, no. 24: 17362. https://doi.org/10.3390/ijms242417362