Genetic Variability in VEGFA Gene Influences the Effectiveness of Tennis Elbow Therapy with PRP: A Two-Year Prospective Cohort Study
Abstract
:1. Introduction
2. Results
2.1. General Characteristics
2.2. Genetic Characteristics
2.3. VEGFA Gene Polymorphisms and the Effectiveness of PRP Therapy
2.4. VEGFA Gene Polymorphisms and Whole Blood and PRP Parameters
2.5. VEGFA Gene Polymorphisms and Clinical Phenotype
3. Discussion
4. Materials and Methods
4.1. Study Design
4.2. Patients
4.3. PRP Separation and Injection Procedure
4.4. Whole Blood and PRP Parameters
4.5. Follow-Up, Outcomes, Measures of Effectiveness
4.6. Genetic Analyses
4.7. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | |||
---|---|---|---|
General | number of subjects, N | 107 | - |
number of elbows, n (%) | 132 | (100.0) | |
tennis elbow in the dominant hand, n (%) | 86 | (65.2) | |
age, median ± QD | 46.00 | 5.50 | |
BMI, median ± QD | 25.65 | 2.00 | |
current smokers, n (%) | 22 | (16.6) | |
Comorbidities | diabetes mellitus, n (%) | 4 | (3.0) |
gout, n (%) | 8 | (6.1) | |
thyroid diseases, n (%) | 15 | (11.4) | |
hypertension, n (%) | 18 | (13.6) | |
Whole Blood | PLT 109/L, median ± QD | 240.00 | 40.50 |
parameters | PCT ml/L, median ± QD | 2.31 | 0.36 |
MPV fL, median ± QD | 9.10 | 0.73 | |
PDW fL, median ± QD | 16.10 | 0.15 | |
PRP parameters | PLT 109/L, median ± QD | 343.00 | 65.00 |
PCT ml/L, median ± QD | 0.30 | 0.06 | |
MPV fL, median ± QD | 8.60 | 0.40 | |
PDW fL, median ± QD | 14.60 | 0.25 |
SNP | Chromosome 6 Coordinate (GRCh37) | Genotypes | n (%) | Alleles | n (%) | p Value HWE Test |
---|---|---|---|---|---|---|
rs699947 | 43736389 | AA | 29 (21.97) | A | 127 (48.11) | 0.923 |
AC | 69 (52.27) | C | 137 (51.89) | |||
CC | 34 (25.76) | |||||
AA + AC | 98 (74.24) | |||||
AC + CC | 103 (78.03) | |||||
rs2010963 | 43738350 | CC | 10 (7.58) | C | 76 (28.79) | 0.978 |
CG | 56 (42.42) | G | 188 (71.21) | |||
GG | 66 (50.00) | |||||
CC + CG | 66 (50.00) | |||||
CG + GG | 122 (92.42) | |||||
rs1413711 | 43740678 | CC | 68 (51.51) | C | 170 (64.39) | 0.000 |
CT | 34 (25.76) | T | 94 (35.61) | |||
TT | 30 (22.73) | |||||
CC + CT | 102 (77.27) | |||||
CT + TT | 64 (48.49) | |||||
rs3024998 | 43745577 | CC | 65 (49.24) | C | 187 (70.83) | 0.916 |
CT | 57 (43.18) | T | 77 (29.17) | |||
TT | 10 (7.58) | |||||
CC + CT | 122 (92.42) | |||||
CT + TT | 67 (50.76) | |||||
rs3025021 | 43749163 | CC | 52 (39.39) | C | 174 (65.91) | 0.246 |
CT | 70 (53.03) | T | 90 (34.09) | |||
TT | 10 (7.58) | |||||
CC + CT | 122 (92.42) | |||||
CT + TT | 80 (60.61) |
SNP | Block Size (kb) | Frequency (%) | |||
---|---|---|---|---|---|
rs699947 | rs2010963 | rs1413711 | rs3024998 | ||
A | G | T | C | 9 | 0.500 |
C | C | C | T | 0.271 | |
C | G | C | C | 0.210 | |
C | G | T | C | 0.005 | |
A | C | C | T | 0.005 | |
C | C | T | C | 0.005 | |
C | G | C | T | 0.005 | |
A | - | T | - | 4 | 0.500 |
C | - | C | - | 0.486 | |
C | - | T | - | 0.009 | |
A | - | C | - | 0.005 | |
- | G | - | C | 7 | 0.715 |
- | C | - | T | 0.276 | |
- | C | - | C | 0.005 | |
- | G | - | T | 0.005 |
PROM | Week | Median ± QD in Respective Genotypes | p Value | |||||
---|---|---|---|---|---|---|---|---|
rs2010963 | Kruskal–Wallis | CC vs. CG | CC vs. GG | CG vs. GG | ||||
CC | CG | GG | ||||||
QDASH | 12 | 36.36 ± 18.18 | 30.68 ± 16.48 | 20.45 ± 20.45 | 0.029 * | 1.000 | 1.000 | 0.024 |
104 | 1.14 ± 25.00 | 22.73 ± 15.91 | 4.55 ± 12.50 | 0.017 * | 0.477 | 1.000 | 0.018 | |
ΔQDASH | 12 | 15.91 ± 9.20 | 14.77 ± 15.34 | 27.27 ± 19.32 | 0.030 * | 1.000 | 1.000 | 0.024 |
104 | 27.27 ± 29.09 | 22.72 ± 16.92 | 40.90 ± 18.19 | 0.023 * | 1.000 | 0.934 | 0.020 | |
PRTEE | 104 | 0.00 ± 21.75 | 14.75 ± 12.50 | 2.50 ± 8.25 | 0.022 * | 0.510 | 1.000 | 0.025 |
rs3024998 | Kruskal–Wallis | CC vs. CT | CC vs. TT | CT vs. TT | ||||
CC | CT | TT | ||||||
QDASH | 12 | 22.73 ± 20.45 | 29.55 ± 17.05 | 36.36 ± 18.18 | 0.043 * | 0.037 | 1.000 | 1.000 |
104 | 4.55 ± 12.50 | 22.73 ± 19.32 | 1.14 ± 25.00 | 0.040 * | 0.047 | 1.000 | 0.575 | |
PRTEE | 24 | 10.00 ± 17.25 | 20.00 ± 17.00 | 24.25 ± 20.50 | 0.048 | 0.043 | 1.000 | 1.000 |
Rs Number | Parameter (Source) | Median | ±QD | Median | ±QD | p |
---|---|---|---|---|---|---|
rs2010963 | GG | CC/CG | ||||
MPV fL (WB) | 9.65 | 0.67 | 8.90 | 0.52 | 0.008 | |
EOS% (WB) | 2.60 | 1.28 | 2.05 | 0.95 | 0.043 | |
rs3024998 | CC | CT/TT | ||||
PLT 109/L (WB) | 227.00 | 38.00 | 261.50 | 32.50 | 0.003 | |
MPV fL (WB) | 9.70 | 0.65 | 8.90 | 0.50 | 0.003 | |
EOS% (WB) | 2.60 | 1.35 | 1.90 | 0.90 | 0.024 | |
MPV fL (PRP) | 8.60 | 0.50 | 8.40 | 0.50 | 0.027 | |
PDW fL (PRP) | 14.60 | 0.20 | 14.50 | 0.20 | 0.030 |
rs2010963 | Parameter | GG | CC/CG | p | ||
median | ±QD | median | ±QD | |||
Alcohol units/week | 1.00 | 4.00 | 0.00 | 2.00 | 0.023 | |
n | % | n | % | |||
Cigarette smoking | 16 | 24.24 | 6 | 9.09 | 0.036 | |
Diabetes mellitus | 0 | 0.00 | 4 | 6.06 | 0.128 | |
rs3024998 | Parameter | CC | CT/TT | p | ||
median | ±QD | median | ±QD | |||
Alcohol units/week | 2.00 | 4.00 | 0.00 | 2.00 | 0.003 | |
n | % | n | % | |||
Cigarette smoking | 16 | 24.62 | 6 | 9.84 | 0.030 | |
Diabetes mellitus | 0 | 0.00 | 4 | 5.97 | 0.136 |
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Niemiec, P.; Jarosz, A.; Balcerzyk-Matić, A.; Iwanicka, J.; Nowak, T.; Iwanicki, T.; Gierek, M.; Kalita, M.; Garczorz, W.; Francuz, T.; et al. Genetic Variability in VEGFA Gene Influences the Effectiveness of Tennis Elbow Therapy with PRP: A Two-Year Prospective Cohort Study. Int. J. Mol. Sci. 2023, 24, 17292. https://doi.org/10.3390/ijms242417292
Niemiec P, Jarosz A, Balcerzyk-Matić A, Iwanicka J, Nowak T, Iwanicki T, Gierek M, Kalita M, Garczorz W, Francuz T, et al. Genetic Variability in VEGFA Gene Influences the Effectiveness of Tennis Elbow Therapy with PRP: A Two-Year Prospective Cohort Study. International Journal of Molecular Sciences. 2023; 24(24):17292. https://doi.org/10.3390/ijms242417292
Chicago/Turabian StyleNiemiec, Paweł, Alicja Jarosz, Anna Balcerzyk-Matić, Joanna Iwanicka, Tomasz Nowak, Tomasz Iwanicki, Marcin Gierek, Marcin Kalita, Wojciech Garczorz, Tomasz Francuz, and et al. 2023. "Genetic Variability in VEGFA Gene Influences the Effectiveness of Tennis Elbow Therapy with PRP: A Two-Year Prospective Cohort Study" International Journal of Molecular Sciences 24, no. 24: 17292. https://doi.org/10.3390/ijms242417292