Quantitative Investigation of FAD2 Cosuppression Reveals RDR6-Dependent and RDR6-Independent Gene Silencing Pathways
Abstract
:1. Introduction
2. Results
2.1. DCL2 and DCL4 Function Redundantly and AGO1 Mediates in FAD2 Cosuppression
2.2. FAD2 Cosuppression Is Enhanced by Mutations in RNA Decay Pathways
3. Discussion
3.1. FAD2 Cosuppression Provides a New Model for the Quantitative Study of RNA Silencing
3.2. FAD2 Cosuppression Shares Similar Genetic Factors with Other Instances of Transgenic Sense Gene Silencing and Endogenous Gene Silencing and also Exhibits an RDR6-Independent Component
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Vector Construction and Plant Transformation
4.3. Fatty Acid Analysis of T1 Single Seeds
4.4. RNA Extraction and Gene Expression Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, Y.; Ku, H.; Zhao, Y.; Du, C.; Zhang, M. Quantitative Investigation of FAD2 Cosuppression Reveals RDR6-Dependent and RDR6-Independent Gene Silencing Pathways. Int. J. Mol. Sci. 2023, 24, 17165. https://doi.org/10.3390/ijms242417165
Chen Y, Ku H, Zhao Y, Du C, Zhang M. Quantitative Investigation of FAD2 Cosuppression Reveals RDR6-Dependent and RDR6-Independent Gene Silencing Pathways. International Journal of Molecular Sciences. 2023; 24(24):17165. https://doi.org/10.3390/ijms242417165
Chicago/Turabian StyleChen, Yangyang, Hangkai Ku, Yingdong Zhao, Chang Du, and Meng Zhang. 2023. "Quantitative Investigation of FAD2 Cosuppression Reveals RDR6-Dependent and RDR6-Independent Gene Silencing Pathways" International Journal of Molecular Sciences 24, no. 24: 17165. https://doi.org/10.3390/ijms242417165