Dual-Specificity Phosphatase 6 Deficiency Attenuates Arterial-Injury-Induced Intimal Hyperplasia in Mice
Abstract
:1. Introduction
2. Results
2.1. DUSP6 Is Increased in the Vessel Wall after Injury and Promotes VSMC Proliferation In Vitro
2.2. Dusp6 Deficiency Reduces Neointima Formation and Decreases VSMC Proliferation
2.3. ERK1/2 Activation Precedes DUSP6 Induction in VSMCs following IL-1β Stimulation
2.4. DUSP6 Regulates Cell Cycle Inhibitor p27 Levels
2.5. Lack of DUSP6 Reduces VSMC Migration
2.6. DUSP6 Deficiency Increases Cell–Cell Adhesion Molecule N-cadherin in VSMCs
3. Discussion
4. Materials and Methods
4.1. Animal Care and Genotyping
4.2. Experimental Mouse Model of Femoral Artery Denudation Injury
4.3. Histological Analysis and Immunohistochemistry
4.4. Primary VSMC Culture, IL-1β Stimulation, and Proliferation Assays
4.5. Wound Healing Assays
4.6. Western Blot Analysis
4.7. DUSP6 Overexpression and Knockdown in VSMCs
4.8. ERK1/2 Signaling and Cell Cycle Inhibitor p27
4.9. Cell Adhesion, Spreading, and Lamellipodia Formation
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hamdin, C.D.; Wu, M.-L.; Chen, C.-M.; Ho, Y.-C.; Jiang, W.-C.; Gung, P.-Y.; Ho, H.-H.; Chuang, H.-C.; Tan, T.-H.; Yet, S.-F. Dual-Specificity Phosphatase 6 Deficiency Attenuates Arterial-Injury-Induced Intimal Hyperplasia in Mice. Int. J. Mol. Sci. 2023, 24, 17136. https://doi.org/10.3390/ijms242417136
Hamdin CD, Wu M-L, Chen C-M, Ho Y-C, Jiang W-C, Gung P-Y, Ho H-H, Chuang H-C, Tan T-H, Yet S-F. Dual-Specificity Phosphatase 6 Deficiency Attenuates Arterial-Injury-Induced Intimal Hyperplasia in Mice. International Journal of Molecular Sciences. 2023; 24(24):17136. https://doi.org/10.3390/ijms242417136
Chicago/Turabian StyleHamdin, Candra D., Meng-Ling Wu, Chen-Mei Chen, Yen-Chun Ho, Wei-Cheng Jiang, Pei-Yu Gung, Hua-Hui Ho, Huai-Chia Chuang, Tse-Hua Tan, and Shaw-Fang Yet. 2023. "Dual-Specificity Phosphatase 6 Deficiency Attenuates Arterial-Injury-Induced Intimal Hyperplasia in Mice" International Journal of Molecular Sciences 24, no. 24: 17136. https://doi.org/10.3390/ijms242417136