Pioglitazone Protects Tubular Epithelial Cells during Kidney Fibrosis by Attenuating miRNA Dysregulation and Autophagy Dysfunction Induced by TGF-β
Abstract
:1. Introduction
2. Results
2.1. Pioglitazone Dampens the TGF-β-Induced Fibrotic Response in Tubular Epithelial Cells
2.2. Pioglitazone Restores the Expression of TGF-β-Induced “fibromiRs”
2.3. Pioglitazone Protects Renal Tubular Cells during Fibrotic Processes by Ameliorating Autophagy Dysfunction
2.4. Pioglitazone Protects Renal Tubular Cells against TGF-β-Induced Inflammation
3. Discussion
4. Materials and Methods
4.1. Primary Tubular Epithelial Cell Isolation and Cell Culture
4.2. Animal Experiments
4.3. Renal Histology and Immunohistochemistry
4.4. Quantitative RT-PCR Analysis
4.5. Immunoblot Analysis
4.6. Immunocytochemistry
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Manzéger, A.; Garmaa, G.; Mózes, M.M.; Hansmann, G.; Kökény, G. Pioglitazone Protects Tubular Epithelial Cells during Kidney Fibrosis by Attenuating miRNA Dysregulation and Autophagy Dysfunction Induced by TGF-β. Int. J. Mol. Sci. 2023, 24, 15520. https://doi.org/10.3390/ijms242115520
Manzéger A, Garmaa G, Mózes MM, Hansmann G, Kökény G. Pioglitazone Protects Tubular Epithelial Cells during Kidney Fibrosis by Attenuating miRNA Dysregulation and Autophagy Dysfunction Induced by TGF-β. International Journal of Molecular Sciences. 2023; 24(21):15520. https://doi.org/10.3390/ijms242115520
Chicago/Turabian StyleManzéger, Anna, Gantsetseg Garmaa, Miklós M. Mózes, Georg Hansmann, and Gábor Kökény. 2023. "Pioglitazone Protects Tubular Epithelial Cells during Kidney Fibrosis by Attenuating miRNA Dysregulation and Autophagy Dysfunction Induced by TGF-β" International Journal of Molecular Sciences 24, no. 21: 15520. https://doi.org/10.3390/ijms242115520