Subacute Ruminal Acidosis as a Potential Factor that Induces Endometrium Injury in Sheep
Abstract
:1. Introduction
2. Results
2.1. High-Concentrate Feed Caused SARA, and Increased LPS and TNFα Concentrations in Rumen Fluid, Serum, and Endometrial Tissue Supernatant in Sheep
2.2. SARA Can Cause the Inflammatory Response of the Endometrium in Sheep
2.3. Effects of SARA on the Endometrial Claudin-1 and Occludin Protein Expression
2.4. Confirmation of Primary Sheep Endometrial Epithelial Cells Cultured In Vitro
2.5. Effects of LPS-Induced Endometrial Epithelial Cell Inflammation on Claudin-1 and Occludin
2.6. Effects of 17β-Estradiol and Progesterone on Epithelial Cell Relative Protein Expression Levels of Claudin-1 and Occludin
2.7. Effect of SARA on the Expression of Endometrial E2 and P4 Receptors
2.8. Mechanism of Effect of SARA on Sheep Endometrial TJs
3. Discussion
4. Materials and Methods
4.1. Establishment of a SARA Model
4.2. Enzyme-Linked Immunosorbent Assay
4.3. Hematoxylin-Eosin Staining
4.4. Immunohistochemical Staining
4.5. Total RNA Isolation and Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
4.6. Western Blotting
4.7. Endometrium Epithelial Cell Culture and Treatment
4.8. Immunofluorescence
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Sequences (5′−3′) | Accession No. |
---|---|---|
TLR4 | F: GGTTTCCACAAGAGCCGTAA | NM_001135930.1 |
R: CTGTTCAGAAGGCGATAGA | ||
NFκB | F: CCAGCATCAAAATCCCCAGC | EF121765.1 |
R: GTGAAGGGTTGGAGACCTCA | ||
TNF–α | F: GGGAACACAGACAGAGGGGACA | EF446377.1 |
R: CCTGCGAGTAGATGAGGTAAAG | ||
ERα | F:GACCGAAGAGGAGGGAGAATG | AY033393.1 |
R:CGGGCTGTTCTTCTTAGTGTGTT | ||
ERβ | F:ACACCTCTCTCCTTTAGCCATCC | AF177936.1 |
R:TCCTTTTCAATGTCTCCCTGTTC | ||
PGR | F:GTCAGGCTGGCATGGTTCTT | Z66555.1 |
R:GGGCTTGGCTTTCATTTGG | ||
β-actin | F: GTCACCAACTTGGGACGACA | U39357 |
R: AGGCGTACAGGGACAGCA |
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Zeng, J.; Lv, J.; Duan, H.; Yang, S.; Wu, J.; Yan, Z.; Zhang, R.; Hu, J.; Zhang, Y. Subacute Ruminal Acidosis as a Potential Factor that Induces Endometrium Injury in Sheep. Int. J. Mol. Sci. 2023, 24, 1192. https://doi.org/10.3390/ijms24021192
Zeng J, Lv J, Duan H, Yang S, Wu J, Yan Z, Zhang R, Hu J, Zhang Y. Subacute Ruminal Acidosis as a Potential Factor that Induces Endometrium Injury in Sheep. International Journal of Molecular Sciences. 2023; 24(2):1192. https://doi.org/10.3390/ijms24021192
Chicago/Turabian StyleZeng, Jianlin, Jianshu Lv, Hongwei Duan, Shuai Yang, Jianxin Wu, Zhenxing Yan, Rong Zhang, Junjie Hu, and Yong Zhang. 2023. "Subacute Ruminal Acidosis as a Potential Factor that Induces Endometrium Injury in Sheep" International Journal of Molecular Sciences 24, no. 2: 1192. https://doi.org/10.3390/ijms24021192