Genome-Wide Identification of MADS-Box Family Genes in Safflower (Carthamus tinctorius L.) and Functional Analysis of CtMADS24 during Flowering
Abstract
:1. Introduction
2. Results
2.1. Phylogenetic Analysis of MADS-Box Family Members
2.2. Analysis of the Conserved Motifs and Gene Structure
2.3. Analysis of Cis-Regulatory Elements in the Promoters of MADS-Box Family Genes
2.4. Gene Expression Analysis by Transcriptome Data of CtMADS during Safflower Floral Development
2.5. Gene Expression Analysis of CtMADS in Different Flower Organs
2.6. Subcellular Location of CtMADS24
2.7. Ectopic Expression of CtMADS24 and Expression Level of Flowering-Related Genes in Arabidopsis
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification of MADS-Box Family Genes
4.3. Phylogenetic Analysis, Conserved Motif, and Gene Structure Analysis of CtMADSs
4.4. Prediction of Cis-Acting Elements in Promoter Sequences
4.5. Safflower MADS-Box Genes Expression Pattern Analysis
4.6. RNA Isolation and Quantitative Real-Time PCR Analysis
4.7. ORF Cloning and Subcellular Localization
4.8. Generation of CtMADS24-Overexpressed Transgenic Arabidopsis Lines and Ectopic Expression Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, Y.; Ge, H.; Ahmad, N.; Li, J.; Wang, Y.; Liu, X.; Liu, W.; Li, X.; Wang, N.; Wang, F.; et al. Genome-Wide Identification of MADS-Box Family Genes in Safflower (Carthamus tinctorius L.) and Functional Analysis of CtMADS24 during Flowering. Int. J. Mol. Sci. 2023, 24, 1026. https://doi.org/10.3390/ijms24021026
Wang Y, Ge H, Ahmad N, Li J, Wang Y, Liu X, Liu W, Li X, Wang N, Wang F, et al. Genome-Wide Identification of MADS-Box Family Genes in Safflower (Carthamus tinctorius L.) and Functional Analysis of CtMADS24 during Flowering. International Journal of Molecular Sciences. 2023; 24(2):1026. https://doi.org/10.3390/ijms24021026
Chicago/Turabian StyleWang, Yifei, Hengshuo Ge, Naveed Ahmad, Jia Li, Yijin Wang, Xinyi Liu, Weican Liu, Xiaowei Li, Nan Wang, Fawei Wang, and et al. 2023. "Genome-Wide Identification of MADS-Box Family Genes in Safflower (Carthamus tinctorius L.) and Functional Analysis of CtMADS24 during Flowering" International Journal of Molecular Sciences 24, no. 2: 1026. https://doi.org/10.3390/ijms24021026