1. Introduction
Recently we reported the crystal structures of cyclic hexameric motifs formed from N
1,N
1′-(butane-1,4-diyl)bis(
N3-butyl-4-nitrobenzene-1,3-diamine)
1, 2,4-bis(phenylamino) nitrobenzene
2 and 2,4-bis(butylamino)nitrobenzene
3 in which adjacent molecules are linked by N—H···(O,O) bifurcated hydrogen bonds (
Figure 1) [
1] or cooperative N—H···O and C—H···O hydrogen bonds (
Figure 2) [
2,
3]. The cyclic hexamers formed from compound
1 and compound
2 are stacked on top of each other so that large one-dimensional channels arise but the cyclic hexamers formed from compound
3 have a staggered stacking arrangement so that channels do not occur in the crystal.
The open frameworks formed from compounds
1 and
2 are examples of porous organic materials comprised of discrete organic molecules between which there are only weak non-covalent interactions. This is quite rare as most organic molecules pack to minimise the void volume [
4]. In contrast there are many examples of metal–organic frameworks (MOFs), which have porosity and building blocks which have some rotational flexibility [
5,
6,
7,
8,
9,
10,
11,
12,
13,
14].
Organic compounds that crystallize with channels have been referred to as ‘organic zeolites’ owing to their structural similarity to aluminosilicates [
15]. Inorganic zeolites have many applications, such as carbon dioxide capture, hydrogen storage, heterogeneous catalysis and molecular separation [
16,
17,
18,
19,
20,
21]. Soluble precursors required to form organic zeolites may include these applications and extend their range because of solution processing, the choice of components and their functionality (
Figure 3) [
4]. We and others are also interested in the properties of organic zeolites crystallised from conformationally flexible building block, of which there are many [
13]. Cyclic amide host
4 forms cylindrical channels 5–8 Å in diameter stabilised by urea–urea hydrogen bonding and aromatic stacking [
22,
23]. The material was able to absorb carbon dioxide. Dipeptides, such as compound
5, crystallise with hydrogen-bonded tubular assemblies forming one-dimensional channels with diameters of 3–5.4 Å. These channels were filled with solvent that could be evacuated by heating [
24,
25]. This dipeptide has substituents that must play a role in its porosity. Rigid trimer
6 forms crystals with empty 1D channels 5 Å in diameter, which can clathrate solvent and organic guest molecules [
26,
27]. Tetra (trimethylsilyl ethynyl) biphenyl
7 crystallises, forming narrow channels in three dimensions that interconnect large internal voids of diameter 11 Å [
28]. The porosity of the de-solvated crystals was shown by hydrogen and nitrogen absorption. Asymmetric calixarene
8 crystallises in a tubular fashion to form two types of void space filled with water molecules. One void space is a 3D network of channels with diameters of 3.9 and 8.5 Å and the other void space consists of spherical cages of 11.2 Å in diameter connected by narrow channels [
29,
30]. The host retained its structural integrity upon the removal of the water molecules. Host
6 is rigid and hosts
4–5 and
7–8 are all comparatively rigid compared to the new class introduced here.
2. Results
The retrosynthesis of compound
1 is shown in
Figure 4. The first disconnection shows that the linker, 1,4-diaminobutane
10, was added last by displacing the fluorine atoms
para to the nitro groups. The next disconnection gives the starting materials 2,4-difluoronitrobenzene
11 and butylamine
12. The first step is faster because of the more reactive and electron-deficient nitro compound
11. The ortho fluorine group of compound
11 is selectively displaced first before the para fluorine group by butylamine
12. Presumably the mesomeric and inductive electron withdrawing effect of the nitro group has a greater influence on the ortho positions. This selectivity is key to the success of the synthesis since mixtures are not formed. Also the second fluorine of the mono-substituted product
9 is deactivated by the conjugation of the amino group into the nitro group. However, it is still sufficiently reactive for the second fluorine to be displaced by an amine under reasonable reaction conditions. Ethanol is a good solvent for these displacement reactions, and at 150 °C in a digestion bomb, the second displacement proceeds within 24 h. In this paper, a change of approach has been explored. The diamine linker (
13,
15 or
10) is added in first with the more reactive 2,4-difluoronitrobenzene
11, which should be efficient because an excess of the linker cannot be used. Secondly, an excess of butylamine is coupled with the less reactive para site. The use of butylamine in the last step came about from previous studies, in which we compared methylamine, ethylamine and propylamine [
1,
2]. The products were much more polar and harder to purify and isolate. Butylamine was the minimum length of chain, which made the products less polar and easy to purify. It is also easily crystallised without disorder, whereas longer chains can be disordered in the X-ray single crystal structure determination. Three linkers were chosen for this study that had different degrees of conformational freedom, which would allow for an exploration of ligand flexibility and porous frameworks. The products were all yellow, with λ
max around 400 nm because of the conjugation of the amines to the nitro groups.
The ortho fluorine atoms are more reactive than the para fluorine atoms due to nucleophilic displacement. They react this way selectively [
1,
2,
3].
Figure 5 shows the reaction of ½ an equivalent of piperazine
13 with 2,4-difluoronitrobenzene
11 followed by treatment with butylamine
12 to give product
14. The NMR data are in the
Supplementary Materials Figures S1 and S2. Compared to product
1, the piperazine unit fixes the distance between the aryl rings but still allows for their rotation. The intermediates involved in the first step in
Figure 5,
Figure 6 and
Figure 7 were not isolated because they are characterised indirectly by the second step. Triethylamine is used to mop up acid in the first step and will be present in the second step, but no more was added.
The crystal structure of compound
14 shows that the asymmetric unit consists of half a molecule, with the complete molecule generated by crystallographic inversion symmetry in the space group P2
1/n. The central piperazine ring adopts its usual chair conformation with the exocyclic N—C bonds in equatorial orientations. The dihedral angle between the piperazine ring (all atoms) and the benzene ring is 49.22 (4)°, and the nitro group is twisted from the plane of the benzene ring by 12.83 (16)°. The
n-butyl chain adopts an extended conformation. In the extended structure of compound 14, cooperative N2—H1···O2 and C3—H3···O1 hydrogen bonds to different O atoms of the same nitro-group acceptor (
Figure 6) generate infinite (101) sheets without identifiable channels or porosity.
Figure 7 shows the synthesis of compound
16. The spacer is longer than piperazine but still has some conformational freedom.
The NMR data for compound 16 are in the
Supplementary Materials Figures S3 and S4. The molecular structure of compound
16 consists of a half-molecule in space group
P2
1/
n with the complete molecule generated by crystallographic inversion symmetry (the inversion centre is at 1–
x, 2–
y, 1–
z for the asymmetric molecule). The dihedral angle between the central benzene ring and the pendant nitrobenzene ring is 52.97 (4)°, and the nitro group is close to the plane of its attached ring (dihedral angle = 3.98 (15)°), with this near coplanarity being reinforced by an intramolecular N1—H1n···O1 hydrogen bond. The C2—N1—C7—C8 torsion angle is –178.12 (11)°, and the pendant
n-butyl chain adopts an extended conformation. In the crystal of compound
16, cooperative N2—H2n···O1 and C5—H5···O1 hydrogen bonds occur (
Figure 8), but unlike compound
14, atom O1 acts as a ‘double acceptor’ for both hydrogen bonds. This also results in (101) sheets of molecules without any identifiable pores or channels.
Figure 9 shows the synthesis of compound
17. The spacer has the most flexibility in these studies. Compound
17 is an isomer of compound
1, in which the positions of the bridging and terminal
n-butyl chains on the nitrobenzene ring are swapped, so here, the two crystal structures can be compared (see below). For compound
1, butylamine reacted firstly in the ortho positions, followed by the linker in the para position. For compound
17, the linker reacts firstly in the ortho positions, followed by butylamine in the para positions. Compound
17 is poorly soluble and is unusually polar compared to its isomer
1. For this reason, only the IR and UV data have been reported, and the crystal structure and it could not be chromatographed with MeOH on silica gel. Compound
1 was purified by chromatography on silica gel with ether/dichloromethane mixture (20:80), which is significantly less polar. The alkylamino-nitro groups in compound
17 must bind to the silica surface or wrap round the silanol groups but they cannot in compound
1.
Compound
17 crystallises with three half-molecules (containing C1, C13 and C25) in the asymmetric unit, with each molecule completed by crystallographic inversion symmetry (
Figure 10); this results in the uncommon situation of
Z = 3 in space group
P. The central butyl chain adopts an extended conformation in each molecule, as does the pendant
n-butyl chain. The dihedral angles between the benzene ring and its attached nitro group are 5.2 (3), 0.8 (3) and 0.9 (5)° for the C1, C13 and C25 molecules, respectively; in each case an intramolecular N—H···O hydrogen bond occurs as also seen in
16. Adjacent molecules in the crystal of compound
17 are linked by cooperative N—H···O and C—H···O hydrogen bonds to different O atoms in an adjacent nitro group in a similar fashion to structure
14. Unlike compounds
14 and
16, the extended structure of compound
17 features an infinite network of hydrogen-bonded six-rings of molecules (
Figure 11) to generate porous (31
) sheets with an approximate atom-to-atom pore diameter of 11.4 Å, but from layer-to-layer, groups of three
n-butyl chains stack on top of a pore, so there is unlikely to be any bulk porosity. A PLATON void-space analysis indicated that 148 Å
3 per unit cell (7.6% of the total unit-cell volume) could be regarded as free space, which is probably occupied by highly disordered solvent molecules.
3. Discussion
Some key structural features of compounds
1,
14,
16 and
17 are summarised in
Table 1.
The key synthon of a group of five atoms consisting of an N—H group (adjacent to the benzene ring) with an ortho C—H group (i.e., H—N—C
ar—C
ar—H; ar = aromatic) as cooperative hydrogen-bond donor(s) and a nitro group as acceptor in these related phases shows notable flexibility with bifurcated N—H···(O,O), double acceptor (N—H,C—H)···O and cooperative N—H···O + C—H····O′ (we call these B, D and C modes, respectively; see
Table 1 footnote and figures above), which are all possible. However, this
motif by itself cannot be directly related to the formation of a dense or porous network, as both compound
14 (dense) and
17 (porous) show the same type-C motif. Conversely, both
1 (B mode) and
17 (C mode) show porosity. As noted previously, compounds
1 and
17 are isomers in which the linker and pendant groups have swapped placed on the nitrobenzene ring. The biggest structural difference lies in the conformation of the linking butyl chain, which is very contorted in compound
1 (
a–
g–
a–
g–
g) (
a = anti,
g = gauche) and centrosymmetric all-anti in compound
17. A major difference between the extended structures of compounds
1 and
17 arises from the different natures of their pores. In compound
1, the [001] channels may be assumed to be hydrophobic, being lined by six
n-butyl chains, but in compound
17, the (31
) pores within a single hydrogen-bonded layer must have a more hydrophilic nature, being lined by oxygen atoms (
Figure 12). As might be expected, the continuous channels in compound
1 show a far greater bulk porosity (2660 Å
3 or 19.8% of the total unit-cell volume) than the separated pores in compound
17 (148 Å
3 or 7.6% of the total unit-cell volume). Other weak intermolecular interactions in these structures should also be briefly considered: in compound
1, there are no significant aromatic π–π stacking interactions (shortest centroid–centroid separation = 4.3271 (14) Å), and any C—H···π bonds must be extremely weak, with the shortest H···π distance being 2.88 Å. In compound
14, the shortest π–π centroid separation of 5.0132 (7) Å is far too long to be regarded as a significant interaction, and the shortest C—H···π bond (2.86 Å) is also very weak. Compound
16 shows corresponding values of 4.9853 (8) Å and 2.73 Å for the π–π centroid separation and C—H···π separations, respectively. Finally, compound
17 has a shortest ring-centroid separation of 4.561 (3) Å and a shortest C—H···π contact of 2.69 Å. To this may be added an N—O···π contact with O···π = 3.244 (5), rather less than the van der Waals radius sum of 3.32 Å (assuming a ‘half-thickness’ of an aromatic ring to be 1.6 Å), and N—O···π = 93.5 (3)°, but the significance of such contacts is debated [
31].
The C—H···O hydrogen bonding observed in compound
17 is unusual because hydrogen is of similar electronegativity to carbon, but it is common in biological systems and has been a topic of discussion [
32,
33,
34,
35,
36].
4. Materials and Methods
IR spectra were recorded on a Thermoscientific Nicolet Summit diamond-attenuated total reflection Everest (ATR) Fourier transform infrared (FTIR) spectrometer (Milton Park, Oxford, UK). Ultraviolet (UV) spectra were recorded using a Perkin Elmer Lambda 25 UV-Vis spectrometer with EtOH as the solvent (Chalfont Road, Buckinghamshire, UK). The term sh means shoulder. 1H and 13C nuclear magnetic resonance (NMR) spectra were recorded at 400 and 100.5 MHz, respectively, using a Bruker 400 spectrometer (Welland House, Coventry, UK). Chemical shifts, δ, are given in ppm and measured by comparison with the residual solvent. Coupling constants, J, are given in Hz. High-resolution mass spectra were obtained at the University of Wales, Swansea, using an Atmospheric Solids Analysis Probe (ASAP) (positive mode) instrument, namely Xevo G2-S ASAP (WatersTM, Wilmslow, UK). Melting points were determined on a Cole-Palmer MP-200D-120 Stuart Digital Melting Point Apparatus; 120 VAC (Cole-Palmer Cambridgeshire, UK).
4.1. Compound 14
2,4-Difluoronitrobenzene (208 mg, 1.3 mmol) and piperazine (56 mg, 0.65 mmol) were mixed with Et3N (132 mg, 1.3 mmol) in EtOH (10 mL) in a Teflon-lined Parr acid digestion bomb. The reactants were heated at 150 °C for 12 h. The vessel was left to cool, and then excess butylamine (191 mg, 2.6 mmol) was added. The reaction vessel was then heated at 150 °C for 12 h again. Once cooled the reaction mixture was diluted with DCM (100 mL) and extracted with water (150 mL) in a separating funnel. The DCM layer was collected then extracted with water (75 mL), dried with MgSO4, then filtered. The product was purified by chromatography on flash silica. Elution with DCM/light petroleum ether (25:75) gave the title compound (62 mg, 13%) as yellow crystals, mp 204–205 °C (from dichloromethane:light petroleum ether). λmax (EtOH)/nm 394 (log ε 4.2); ῦmax (diamond)(cm–1) 3356s, 2932w, 2864w, 1596s, 1259s, 1240s, 1200s, 1104s, 978s, 740s, 523s and 454s; δH (400 MHz; CDCl3) 1.01 (6H, t, J = 8.0), 1.48 (4H, m), 1.67 (4H, m), 3.20 (4H, m), 3.29 (8H, s), 4.81 (2H, NH), 6.16 (2H, s), 6.20 (2H, d, J = 8.0) and 8.08 (2H, d, J = 8.0); δC (100.1 MHz; CDCl3) 13.7, 20.3, 31.0, 43.0, 51.9, 100.8, 105.7, 130.2, 131.4, 150.7 and 153.4; m/z (Orbitrap ASAP) 471.2726 (M+ + H, 100%) C24H34N6O4H requires 471.2720.
4.2. Compound 16
2,4-Difluoronitrobenzene (194 mg, 1.2 mmol); 1,4-bis(aminomethyl)benzene (83 mg, 0.6 mmol); Et3N (134 mg, 1.3 mmol) and EtOH (10 mL) were mixed in a Teflon-lined Parr acid digestion bomb. The reactants were heated at 150 °C for 12 h. The vessel was left to cool, and excess BuNH2 (194 mg, 2.7 mmol) and EtOH (3.5 mL) were added. The reaction vessel was heated at 150 °C for 12 h again. Once cooled, the reaction mixture was diluted with DCM (100 mL) and extracted with water (150 mL) in a separating funnel. The DCM layer was collected then extracted with water (75 mL), dried with MgSO4, then filtered. The product was purified by chromatography on flash silica. Elution with DCM/light petroleum ether (25:75) gave the title compound (59 mg, 19%) as crystals, mp 203–204 °C (from dichloromethane: light petroleum ether). λmax (EtOH)/nm 406 (log ε 4.3); ῦmax (diamond)(cm–1) 3312s, 2956w, 2928w, 2860w, 1616s, 1577s, 1543s, 1458s, 1398s, 1315s, 1251s, 1190s, 1163s, 1129s, 817s, 749s and 556s; δH (400 MHz; CDCl3) 0.94 (6H, t, J = 8.0), 1.37 (4H, m), 1.54 (4H, m), 3.09 (4H, m), 4.39 (2H, m, br), 4.51 (4H, d, J = 4.0), 5.62 (2H, s), 5.93 (2H, d, J = 8.0), 7.37 (4H, s), 8.06 (2H, d, J = 8.0) and 8.93 (2H, m, br); δC (100.1 MHz; CDCl3) 13.8, 20.1, 31.0, 42.9, 46.7, 90.9, 104.8, 123.9, 127.6, 129.4, 137.1, 148.2 and 154.3; m/z (Orbitrap ASAP) 521.2877 (M+ + H, 100%) C28H36N6O4H requires 521.2876.
4.3. Compound 17
2,4-Difluoronitrobenzene (500 mg, 3.14 mmol) in EtOH (30 mL) was mixed with 1,4-diaminobutane (138 mg, 1.57 mmol) and Et3N (318 mg, 3.14 mmol). The reaction was heated at 70 °C, cooled, and filtered. The solid was mixed with BuNH2 (459 mg, 6.28 mmol) in EtOH (10 mL) in a Teflon-lined Parr acid digestion bomb and heated at 150 °C for 18 h. After cooling, the polar yellow crystals were harvested (103 mg, 13%), mp 207–208 °C. λmax (EtOH)/nm 225 (log ε 3.3) and 398 (3.2); ῦmax (diamond)(cm–1) 3318s, 2927w, 2857w, 1615s, 1577s, 1543s, 1460s, 1401s, 1321s, 1247s, 1166s, 815s, 749s, 599s and 533s; δH (400 MHz; CDCl3); m/z (Orbitrap ASAP) 473.2877 (M+ + H, 100%) C24H36N6O4H requires 473.2876.
4.4. Crystal Structures
The crystal structures of compounds
14,
16 and
17 were established using intensity data collected on a Rigaku CCD diffractometer. The structures were routinely solved by dual-space methods using SHELXT [
37], and the structural models were completed and optimized by refinement against |
F|
2 with SHELXL-2019 [
38]. The N-bound hydrogen atom(s) were located in difference maps for compounds
14 and
16, and their positions were freely refined; for compound
20, they were geometrically placed and refined as riding atoms. The C-bound hydrogen atoms were placed in idealized locations (C—H = 0.95–0.99 Å) and were refined as riding atoms. The methyl groups were allowed to rotate, but not to tip, to best fit the electron density. The constraint
Uiso(H) = 1.2
Ueq(carrier) or 1.5
Ueq (methyl carrier) was applied in all cases. The data quality for compound
17 is poorer, perhaps because of some loss of disordered incorporated solvent during data collection, but the structure has been unambiguously established. Full details of the structures and refinements are available in the deposited cifs and CCDC numbers.
Crystal data for 14 C24H34N6O4, pale yellow rod 0.17 × 0.06 × 0.02 mm, Mr = 470.56, monoclinic, space group P21/n (No. 14), a = 8.1792 (2) Å, b = 11.7954 (5) Å, c = 12.5512 (4) Å, β = 94.089 (3)°, V = 1207.82 (7) Å3, Z = 2, T = 100 K, Cu Kα radiation, λ = 1.54178 Å, μ = 0.734 mm–1, ρcalc = 1.294 g cm–3, 11,714 reflections measured (10.3 ≤ 2θ ≤ 149.9°), 2422 unique (RInt = 0.035), R(F) = 0.035 [2062 reflections with I > 2σ(I)], wR(F2) = 0.095 (all data), Δρmin,max (e Å–3) = –0.18, +0.28, CCDC deposition number 2293919.
Crystal data for 16 C28H36N6O4, pale yellow prism 0.24 × 0.20 × 0.14 mm, Mr = 520.63, monoclinic, space group P21/n (No. 14), a = 7.1262 (2) Å, b = 14.9575 (6) Å, c = 12.5881 (6) Å, β = 99.183 (4)°, V = 1324.57 (9) Å3, Z = 2, T = 100 K, Cu Kα radiation, λ = 1.54178 Å, μ = 0.724 mm–1, ρcalc = 1.305 g cm–3, 13,406 reflections measured (9.3 ≤ 2θ ≤ 153.6°), 2736 unique (RInt = 0.048), R(F) = 0.040 [2473 reflections with I > 2σ(I)], wR(F2) = 0.107 (all data), Δρmin,max (e Å–3) = –0.20, +0.28, CCDC deposition number 2293920.
Crystal data for 17 C24H36N6O4, very pale yellow chip 0.07 × 0.03 × 0.02 mm, Mr = 472.59, triclinic, space group P(No. 2), a = 10.3217 (10) Å, b = 14.5785 (12) Å, c = 15.4065 (14) Å, α = 116.407 (9)°, β = 105.981 (8)°, γ = 92.447 (7)°, V = 1959.1 (3) Å3, Z = 3, T = 293 K, synchrotron radiation, λ = 0.6889 Å, μ = 0.079 mm–1, ρcalc = 1.202 g cm–3, 26,619 reflections measured (3.0 ≤ 2θ ≤ 47.0°), 6236 unique (RInt = 0.181), R(F) = 0.144 [2989 reflections with I > 2σ(I)], wR(F2) = 0.389 (all data), Δρmin,max (e Å–3) = –0.39, +0.66, CCDC deposition number 2293921.