Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed
Abstract
:1. Introduction
2. Results
2.1. Expression of GmCGS2 Was Significantly Higher Than that of GmCGS1 at All Seed Developmental Stages
2.2. Transgenic Soybean Exhibited Elevated Levels of CGS during Seed Development
2.3. Overexpression of GmCGS2 Increased the Content of Free Met and Total Free Amino Acids
2.4. Overexpression of GmCGS2 Increased the Content of Total Protein-Bound Amino Acids and Soluble Protein but Not Protein-Bound Met
2.5. Global Transcriptome Responses of Developing Soybean Seeds after Overexpression of GmCGS2
2.6. Overexpression of GmCGS2 Positively Regulated the Aspartate-Family Pathway and SMM Cycling
2.7. GmCGS2 Expression May Be Related to Transcription Factors Responding to Biotic and Abiotic Stressors
3. Discussion
4. Materials and Methods
4.1. Phylogenetic Analysis
4.2. Plants and Growth Conditions
4.3. Quantitative Reverse-Transcription (qRT)-PCR Analysis
4.4. Subcellular Localization Analysis
4.5. Plasmid Construction, Plant Transformation, and Screening
4.6. Quantifications of Amino Acid and Soluble Protein Contents
4.7. Library Preparation and RNA Sequencing
4.8. RNA-seq Data Analysis
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, Y.; Wang, Q.; Liu, Y.; Dong, S.; Zhang, Y.; Zhu, Y.; Tian, Y.; Li, J.; Wang, Z.; Wang, Y.; et al. Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed. Int. J. Mol. Sci. 2023, 24, 14125. https://doi.org/10.3390/ijms241814125
Zhang Y, Wang Q, Liu Y, Dong S, Zhang Y, Zhu Y, Tian Y, Li J, Wang Z, Wang Y, et al. Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed. International Journal of Molecular Sciences. 2023; 24(18):14125. https://doi.org/10.3390/ijms241814125
Chicago/Turabian StyleZhang, Yuchen, Qingyu Wang, Yajing Liu, Shuo Dong, Yongqiang Zhang, Youcheng Zhu, Yu Tian, Jingwen Li, Zhuoyi Wang, Ying Wang, and et al. 2023. "Overexpressing GmCGS2 Improves Total Amino Acid and Protein Content in Soybean Seed" International Journal of Molecular Sciences 24, no. 18: 14125. https://doi.org/10.3390/ijms241814125