Staphylococcal Enterotoxin C2 Mutant-Induced Antitumor Immune Response Is Controlled by CDC42/MLC2-Mediated Tumor Cell Stiffness
Abstract
:1. Introduction
2. Results
2.1. Analysis of SEC2/ST-4-Stimulated PBMC Activation and the Production of Cytotoxic Factors
2.2. Antitumor Activity of SEC2/ST-4 in Ovarian Cancer Cells
2.3. Transcriptome Analysis after Treatment in SEC2/ST-4 Condition
2.4. Analysis of Cell Stiffness and Regulatory Molecules in Ovarian Cancer Cells
2.5. Importance of CDC42 Signaling for SEC2/ST-4-Induced Antitumor Activity
2.6. Increased Cell Stiffness Improves SEC2/ST-4-Induced Antitumor Activity
2.7. SEC2/ST-4-Induced S Phase Arresting and Apoptosis in Blebbistatin-Treated Stiffer Ovarian Cancer Cells
2.8. SEC2/ST-4-Induced Blebbistatin-Treated Stiffer Ovarian Cancer Cells Apoptosis through Caspase Signaling
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Lines, Cell Isolation, and Culture
4.3. CFSE Proliferation Assay
4.4. Cytokine Assay
4.5. RNA-Sequencing and DEGs Analysis
4.6. Transfection of CDC42 siRNA
4.7. Antitumor Assay
4.8. Immunoflourescence Staining
4.9. Measurement of Single Cell Stiffness
4.10. Transwell Assay
4.11. Evaluation of Cell Cycle Distribution and Cell Apoptosis by Flow Cytometry
4.12. Western Blotting
4.13. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fu, X.; Xu, M.; Yu, Z.; Gu, W.; Zhang, Z.; Zhang, B.; Wang, X.; Su, Z.; Zhang, C. Staphylococcal Enterotoxin C2 Mutant-Induced Antitumor Immune Response Is Controlled by CDC42/MLC2-Mediated Tumor Cell Stiffness. Int. J. Mol. Sci. 2023, 24, 11796. https://doi.org/10.3390/ijms241411796
Fu X, Xu M, Yu Z, Gu W, Zhang Z, Zhang B, Wang X, Su Z, Zhang C. Staphylococcal Enterotoxin C2 Mutant-Induced Antitumor Immune Response Is Controlled by CDC42/MLC2-Mediated Tumor Cell Stiffness. International Journal of Molecular Sciences. 2023; 24(14):11796. https://doi.org/10.3390/ijms241411796
Chicago/Turabian StyleFu, Xuanhe, Mingkai Xu, Zhixiong Yu, Wu Gu, Zhichun Zhang, Bowen Zhang, Xiujuan Wang, Zhencheng Su, and Chenggang Zhang. 2023. "Staphylococcal Enterotoxin C2 Mutant-Induced Antitumor Immune Response Is Controlled by CDC42/MLC2-Mediated Tumor Cell Stiffness" International Journal of Molecular Sciences 24, no. 14: 11796. https://doi.org/10.3390/ijms241411796