A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

Round 1

Reviewer 1 Report

The research paper describes a novel mannose receptor LvMR containing a FN II domain and two CRDs found in L. vannamei. The article shows great information as it revealed that this receptor was predominantly expressed in hepato- pancreas and that it responded to V. parahaemolyticus and WSSV infection. The results obtained in this article suggest that LvMR may participate in immune recognition and anti-inflammatory response in the innate immunity of L. vanname. The results are novel and can be further used in future researches explaining the mechanisms of inflammatory response. It deserves to be published in current form. The methodology applied is very detailed and the techniques used are appropriate. 

Reviewer 2 Report

A novel mannose receptor LvMR containing a FN II domain and two CRDs was identified from L. vannamei.

LvMR was predominantly expressed in hepatopancreas and clearly responded to V. parahaemolyticus and WSSV infection.

The recombinant LvMR had significant binding and agglutinating activities to PAMPs or pathogens. LvMR with mutated EPS motif had more diverse carbohydrate recognition specificity.

LvMR knockdown could upregulate the expression of most inflammation-related genes and antioxidant enzymes, and enhance the respiratory burst of hepatopancreas induced by V. parahaemolyticus infection by the increase of ROS content and iNOS activity.

All these results suggest that LvMR may participate in immune recognition and anti-inflammatory response in the innate immunity of L. vannamei.

Comments for author File: Comments.docx

Reviewer 3 Report

 

Dear authors! Your work is highly commendable. I wish you continued success in this direction. Note the involvement of hydrosulfides (SSnH) as regulators of receptor activity.