Overexpression of 9-cis-Epoxycarotenoid Dioxygenase Gene, IbNCED1, Negatively Regulates Plant Height in Transgenic Sweet Potato
Abstract
:1. Introduction
2. Results
2.1. Cloning and Sequence Analysis of IbNCED1
2.2. Expression Analysis of IbNCED1
2.3. Regeneration of the Transgenic Sweet Potato Plants
2.4. Plant Height Assay
2.5. Underlying Mechanism of IbNCED1 in Plant Height
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Cloning and Sequence Analysis of IbNCED1
4.3. Expression Analysis of IbNCED1
4.4. Regeneration of the Transgenic Sweet Potato Plants
4.5. Plant Height Analysis
4.6. RNA-Sequencing and Hormone Analysis
4.7. Exogenous GA3 Treatment Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Class | Index | WT | L2 |
---|---|---|---|
ABA | ABA | 7.60 ± 0.68 | 83.42 ± 1.77 |
ABA-GE | 133.91 ± 4.82 | 355.59 ± 8.22 | |
GA | GA1 | N/A | N/A |
GA3 | 5.02 ± 0.16 | N/A | |
GA4 | N/A | N/A | |
GA7 | N/A | N/A | |
GA9 | N/A | N/A | |
GA15 | N/A | N/A | |
GA19 | 21.15 ± 0.53 | 23.85 ± 0.84 | |
GA20 | N/A | N/A | |
GA24 | N/A | N/A | |
GA53 | 6.36 ±0.68 | 11.34 ± 0.30 |
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Zhou, Y.; Zhao, C.; Du, T.; Li, A.; Qin, Z.; Zhang, L.; Dong, S.; Wang, Q.; Hou, F. Overexpression of 9-cis-Epoxycarotenoid Dioxygenase Gene, IbNCED1, Negatively Regulates Plant Height in Transgenic Sweet Potato. Int. J. Mol. Sci. 2023, 24, 10421. https://doi.org/10.3390/ijms241310421
Zhou Y, Zhao C, Du T, Li A, Qin Z, Zhang L, Dong S, Wang Q, Hou F. Overexpression of 9-cis-Epoxycarotenoid Dioxygenase Gene, IbNCED1, Negatively Regulates Plant Height in Transgenic Sweet Potato. International Journal of Molecular Sciences. 2023; 24(13):10421. https://doi.org/10.3390/ijms241310421
Chicago/Turabian StyleZhou, Yuanyuan, Chunling Zhao, Taifeng Du, Aixian Li, Zhen Qin, Liming Zhang, Shunxu Dong, Qingmei Wang, and Fuyun Hou. 2023. "Overexpression of 9-cis-Epoxycarotenoid Dioxygenase Gene, IbNCED1, Negatively Regulates Plant Height in Transgenic Sweet Potato" International Journal of Molecular Sciences 24, no. 13: 10421. https://doi.org/10.3390/ijms241310421