Molecular Detection of Toxoplasma gondii, Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Tested Animals
4.2. Methods of Molecular Biology
4.3. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristic | Animal Species | Total | ||
---|---|---|---|---|
Myotis myotis | Nyctalus noctula | Vespertilio murinus | ||
Localities | p = 0.0409 | p = 0.2258 | p > 0.9999 | |
Czech Republic | 22/50 (44%) | 2/35 (6%) | 1/5 (20%) | 25/90 (28%) |
Central Bohemian | 1/5 (20%) | 0/10 (0%) | 1/2 | 2/17 (12%) |
Moravian-Silesian | 1/7 (14%) | – | – | 1/7 (14%) |
Plzeň | 0/1 | 0/2 | – | 0/3 |
Prague | – | 1/5 (20%) | 0/1 | 1/6 (17%) |
South Moravia | 19/32 (59%) | 0/6 (0%) | 0/2 | 19/40 (48%) |
No information | 1/5 (20) | 1/12 (8%) | 0 | 2/17 (12%) |
Slovak Republic | 0/2 | 0/8 (0%) | 0/0 | 0/10 (0%) |
Gender | p > 0.5234 | p > 0.9999 | p = 0.4000 | |
Female | 10/21 (48%) | 1/27 (4%) | 1/2 | 12/50 (24%) |
Male | 12/31 (39%) | 1/16 (6%) | 0/3 | 13/50 (26%) |
Total | 22/52 (42%) | 2/43 (5%) | 1/5 (20%) | 25/100 (25%) |
Primer Name | Oligonucleotide Sequences |
---|---|
Encephalitozoon cuniculi (ITS region) | |
MSP 1 | 5′ TGA ATG (G,T)GT CCC TGT 3′ |
MSP 2A | 5′ TCA CTC GCC GCT ACT 3′ |
MSP 3 | 5′ GGA ATT CAC ACC GCC CGT C(A,G)(C,T) TAT 3′ |
MSP 4A | 5′ CCA AGC TTA TGC TTA AGT (C,T)(A,C)A A(A,G)G GG 3′ |
Neospora caninum (Nc-5 region) | |
Np21+ | 5′ CCCAGTGCGTCCAATCCTGTAAC 3′ |
Np6+ | 5′ CTCGCCAGTCAACCTACGTCTTCT 3′ |
Toxoplasma gondii (529-bp repeated element) | |
TOX4 | 5′ CTGCAGGGAG GAAGACGAAA GTTG 3′ |
TOX5 | 5′ CGCTGCAGACACAGTGCATCTGGATT 3′ |
Marker | Primer Sequence |
---|---|
TUB2 | 5′ 6-FAM-GTCCGGGTGTTCCTACAAAA 3′ |
5′ TTGGCCAAAGACGAAGTTGT 3′ | |
W35 | 5′ HEX-GGTTCACTGGATCTTCTCCAA 3′ |
5′ AATGAACGTCGCTTGTTTCC 3′ | |
TgM-A | 5′ HEX-GGCGTCGACATGAGTTTCTC 3′ |
5′ TGGGCATGTAAATGTAGAGATG 3′ | |
B18 | 5′ 6-FAM-TGGTCTTCACCCTTTCATCC 3′ |
5′ AGGGATAAGTTTCTTCACAACGA 3′ | |
B17 | 5′ HEX-AACAGACACCCGATGCCTAC 3′ |
5′ GGCAACAGGAGGTAGAGGAG 3′ | |
M33 | 5′ 6-FAM-TACGCTTCGCATTGTACCAG 3′ |
5′ TCTTTTCTCCCCTTCGCTCT 3′ | |
IV.1 | 5′ HEX-GAAGTTCGGCCTGTTCCTC 3′ |
5′ TCTGCCTGGAAAAGGAAAGA 3′ | |
XI.1 | 5′ 6-FAM-GCGTGTGACGAGTTCTGAAA 3′ |
5′ AAGTCCCCTGAAAAGCCAAT 3′ | |
M48 | 5′ 6-FAM-AACATGTCGCGTAAGATTCG 3′ |
5′ CTCTTCACTGAGCGCCTTTC 3′ | |
M102 | 5′ NED-CAGTCCAGGCATACCTCACC 3′ |
5′ CAATCCCAAAATCCCAAACC 3′ | |
N60 | 5′ NED-GAATCGTCGAGGTGCTATCC 3′ |
5′ AACGGTTGACCTGTGGCGAGT 3′ | |
N82 | 5′ HEX-TGCGTGCTTGTCAGAGTTC 3′ |
5′ GCGTCCTTGACATGCACAT 3′ | |
AA | 5′ NED-GATGTCCGGTCAATTTTGCT 3′ |
5′ GACGGGAAGGACAGAAACAC 3′ | |
N61 | 5′ 6-FAM-ATCGGCGGTGGTTGTAGAT 3′ |
5′ CCTGATGTTGATGTAAGGATGC 3′ | |
N83 | 5′ 6-FAM-ATGGGTGAACAGCGTAGACA 3′ |
5′ GCAGGACGAAGAGGATGAGA 3′ |
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Bártová, E.; Marková, J.; Sedláčková, J.; Banďouchová, H.; Račka, K. Molecular Detection of Toxoplasma gondii, Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe. Int. J. Mol. Sci. 2023, 24, 9887. https://doi.org/10.3390/ijms24129887
Bártová E, Marková J, Sedláčková J, Banďouchová H, Račka K. Molecular Detection of Toxoplasma gondii, Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe. International Journal of Molecular Sciences. 2023; 24(12):9887. https://doi.org/10.3390/ijms24129887
Chicago/Turabian StyleBártová, Eva, Jiřina Marková, Jana Sedláčková, Hana Banďouchová, and Karol Račka. 2023. "Molecular Detection of Toxoplasma gondii, Neospora caninum and Encephalitozoon spp. in Vespertilionid Bats from Central Europe" International Journal of Molecular Sciences 24, no. 12: 9887. https://doi.org/10.3390/ijms24129887