Bacillus velezensis RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits
Abstract
:1. Introduction
2. Results
2.1. Isolation and Screening of Rhizosphere Bacteria with Antibacterial Activity against Rs
2.2. RC116 with Broad-Spectrum Antimicrobial Activity Was Identified as B. velnzensis
2.3. RC116 and the Biocontrol Reference Strain FZB42 Show Stronger Antibacterial Activity Compared with Two Other B. velezensis Strains
2.4. Genome-Based Functional Annotation and Molecular Biology Assays Revealed the Antimicrobial Potential of RC116
2.5. Extracellular Enzymes of RC116 Exhibited Considerable Lytic Activity against Rs and Fol In Vitro
2.6. RC116 Exhibited High Biocontrol Efficacy against TBW and Promoted the Growth of Tomato Plantlets
3. Discussion
4. Materials and Methods
4.1. Antagonistic Bacteria and Plant Pathogens
4.2. Strain Isolation and Growth Conditions
4.3. Identification of RC116
4.4. DNA Extraction, Genome Sequencing, and Genome Assembly
4.5. Genome Function Annotation and Bioinformatic Analysis
4.6. Evaluation of the Ability of RC116 Antagonistic Plant Pathogens
4.7. Evaluation of the Biocontrol Efficacy and Growth-Promoting Effects of RC116 on TBW
4.8. Enzyme Productiony and Growth-Promoting Index of RC116
4.9. PCR Detection of Biocontrol-Related Genes in RC116
4.10. Activity of RC116 Extracellular Proteins against Rs and Fol
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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API 20NE | RC116 | API ZYM | RC116 |
---|---|---|---|
Reduction of nitrate to nitrite | + | Control | |
Denitrification | − | Alkaline phosphatase | + |
Indole production | − | Esterase (C4) | + |
D-glucose fermentation | − | Esterase lipase (C8) | + |
Arginine dihydrolase | − | Lipase (C14) | + |
Urease | w | Leucine arylamidase | − |
β-glucosidase (aesculin hydrolysis) | + | Valine arylamidase | − |
Gelatin hydrolysis | + | Cystine arylamidase | − |
D-glucose | + | Trypsin | − |
L-arabinose | + | α-chymotrypsin | − |
D-mannose | + | Acid phosphatase | + |
D-mannitol | + | Napthol-AS-BI-phosphohydrolase | + |
N-acetyl-glucosamine | + | α-galactosidase | − |
D-maltose | + | β-galactosidase | − |
Potassium gluconate | w | β-glucuronidase | − |
Capric acid | − | α-glucosidase | + |
Adipic acid | w | β-glucosidase | + |
Malic acid | + | N-acetyl-β-glucosaminidase | − |
Trisodium citrate | + | α-mannosidase | − |
Phenylacetic acid | w | α-fucosidase | − |
Genomic Features | RC116 |
---|---|
Size (bp) | 4,020,633 |
Contigs numbers | 168 |
DNA G + C content (%) | 46.5 |
The longest contigs (bp) | 2,162,881 |
Contigs N50 (bp) | 2,162,881 |
L 50 value | 1 |
Number of genes | 4044 |
Number of CDS * | 3793 |
Number of rRNA genes | 13 |
Number of tRNA genes | 85 |
Completeness (%) | 99.81 |
Contamination | 0.72 |
GenBank accession number | JAJJMX010000001 |
Bacillus velezensis RC116 | Presence (+) or Absence (−) | ||||
---|---|---|---|---|---|
Gene Clusters | Region | Types | Most Similar Known Clusters | Similarity | FZB 42 |
Scaffold 1 | 1.1 | PKS-like 1 | butirosin A/butirosin B | 7% | + |
1.2 | terpene | unkonwn | − | + | |
1.3 | transAT-PKS, T3PKS 2, NRPS 3 | kalimantacin A | 17% | − | |
1.4 | transAT-PKS | macrolactin H | 100% | + | |
1.5 | transAT-PKS, T3PKS, NRPS | bacillaene | 100% | + | |
1.6 | NRPS, transAT-PKS, betalactone 4 | fengycin | 100% | + | |
1.7 | terpene | unknown | − | + | |
1.8 | T3PKS | micrococcin P1 | 8% | − | |
1.9 | transAT-PKS | difficidin | 100% | + | |
Scaffold 2 | 2.1 | NRPS, Ripp-like 5 | bacillibactin | 100% | + |
2.2 | Other 6 | baciysin | 100% | + | |
Scaffold 3 | 3.1 | NRPS | surfactin | 91% | + |
Scaffold 4 | 4.1 | LAP, RRE-containing 7 | plantazolicin | 91% | + |
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Dong, H.; Gao, R.; Dong, Y.; Yao, Q.; Zhu, H. Bacillus velezensis RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits. Int. J. Mol. Sci. 2023, 24, 8527. https://doi.org/10.3390/ijms24108527
Dong H, Gao R, Dong Y, Yao Q, Zhu H. Bacillus velezensis RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits. International Journal of Molecular Sciences. 2023; 24(10):8527. https://doi.org/10.3390/ijms24108527
Chicago/Turabian StyleDong, Honghong, Ruixiang Gao, Yijie Dong, Qing Yao, and Honghui Zhu. 2023. "Bacillus velezensis RC116 Inhibits the Pathogens of Bacterial Wilt and Fusarium Wilt in Tomato with Multiple Biocontrol Traits" International Journal of Molecular Sciences 24, no. 10: 8527. https://doi.org/10.3390/ijms24108527