Molecular Characterization of Three Tandemly Located Flagellin Genes of Stenotrophomonas maltophilia
Abstract
:1. Introduction
2. Results
2.1. Bioinformatics Analysis of Flagellin Genes
2.2. Impact of Filaments with Different Flagellin Combinations on Swimming, Swarming, Flagellum Morphology, Adhesion, and Biofilm Formation
2.3. Impact of Filaments with Different Flagellin Combinations on Swimming Motility under Different Environmental Conditions
2.4. Phylogenetic Analysis of Flagellin
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Plasmids, and Primers
4.2. Reverse Transcription PCR
4.3. Construction of Promoter-xylE Transcriptional Fusion Constructs
4.4. Catechol 2,3-Dioxygenase (C23O) Activity Determination
4.5. Construction of in-Frame Deletion Mutants
4.6. Swimming Motility Assay
4.7. Swarming Motility Assay
4.8. Flagella staining and TEM observation
4.9. Adhesion on Abiotic Surfaces
4.10. Biofilm Formation on Abiotic Surfaces
4.11. Adhesion and Biofilm Formation on Biotic Surfaces
4.12. Bioinformatics Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wu, C.-M.; Huang, H.-H.; Li, L.-H.; Lin, Y.-T.; Yang, T.-C. Molecular Characterization of Three Tandemly Located Flagellin Genes of Stenotrophomonas maltophilia. Int. J. Mol. Sci. 2022, 23, 3863. https://doi.org/10.3390/ijms23073863
Wu C-M, Huang H-H, Li L-H, Lin Y-T, Yang T-C. Molecular Characterization of Three Tandemly Located Flagellin Genes of Stenotrophomonas maltophilia. International Journal of Molecular Sciences. 2022; 23(7):3863. https://doi.org/10.3390/ijms23073863
Chicago/Turabian StyleWu, Cheng-Mu, Hsin-Hui Huang, Li-Hua Li, Yi-Tsung Lin, and Tsuey-Ching Yang. 2022. "Molecular Characterization of Three Tandemly Located Flagellin Genes of Stenotrophomonas maltophilia" International Journal of Molecular Sciences 23, no. 7: 3863. https://doi.org/10.3390/ijms23073863