Next Article in Journal
Sevoflurane Effects on Neuronal Energy Metabolism Correlate with Activity States While Mitochondrial Function Remains Intact
Next Article in Special Issue
GWAS Reveals a Novel Candidate Gene CmoAP2/ERF in Pumpkin (Cucurbita moschata) Involved in Resistance to Powdery Mildew
Previous Article in Journal
Asian Sand Dust Particles Enhance the Development of Aspergillus fumigatus Biofilm on Nasal Epithelial Cells
Previous Article in Special Issue
Breeding and Genomics Interventions for Developing Ascochyta Blight Resistant Grain Legumes
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 6
10.3390/ijms23063031
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Function of Nuclear Pore Complexes in Regulation of Plant Defense Signaling

by
Xi Wu
1,
Junyou Han
1,* and
Changkui Guo
2,*
1
Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, China
2
Laboratory of Plant Molecular and Developmental Biology, Zhejiang A & F University, Hangzhou 311300, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(6), 3031; https://doi.org/10.3390/ijms23063031
Submission received: 2 February 2022 / Revised: 6 March 2022 / Accepted: 9 March 2022 / Published: 11 March 2022
(This article belongs to the Special Issue Structural and Functional Genomics for Plant Development and Stress Tolerance)
Browse Figure
Versions Notes

Abstract

:
In eukaryotes, the nucleus is the regulatory center of cytogenetics and metabolism, and it is critical for fundamental biological processes, including DNA replication and transcription, protein synthesis, and biological macromolecule transportation. The eukaryotic nucleus is surrounded by a lipid bilayer called the nuclear envelope (NE), which creates a microenvironment for sophisticated cellular processes. The NE is perforated by the nuclear pore complex (NPC), which is the channel for biological macromolecule bi-directional transport between the nucleus and cytoplasm. It is well known that NPC is the spatial designer of the genome and the manager of genomic function. Moreover, the NPC is considered to be a platform for the continual adaptation and evolution of eukaryotes. So far, a number of nucleoporins required for plant-defense processes have been identified. Here, we first provide an overview of NPC organization in plants, and then discuss recent findings in the plant NPC to elaborate on and dissect the distinct defensive functions of different NPC subcomponents in plant immune defense, growth and development, hormone signaling, and temperature response. Nucleoporins located in different components of NPC have their unique functions, and the link between the NPC and nucleocytoplasmic trafficking promotes crosstalk of different defense signals in plants. It is necessary to explore appropriate components of the NPC as potential targets for the breeding of high-quality and broad spectrum resistance crop varieties.

1. Introduction

The nuclear envelope (NE) is the key distinguishing factor between eukaryotes and prokaryotes, and it compartmentalizes a microenvironment for dedicated biological processes including genomic DNA replication and repair, transcription, and RNA processing. The NE is embedded with numerous small holes called nuclear pores, through which the largest and sophisticated protein complex, the nuclear pore complex (NPC), penetrates. The NPC mediates the selective transport of macromolecules, and allows for the passive transport of molecular cargos smaller than 40 kDa. Activating the transport of larger molecules requires the assistance of nuclear transport receptors (NTRs) [1,2].
The NPC is composed of approximately 30 kinds of NUPs [1,2]. Different NUPs are arranged in distinct NPC subcomplexes and perform unique cellular processes [1]. In addition to their basic function in facilitating the transport of biomolecules between the nucleus and cytoplasm [3], NPC also function in regulating the spatial location of chromatin and RNA processing in a transport-independent manner [4,5,6,7]. The spatial segregation of euchromatin and heterochromatin in nuclei is not a random event [8,9]. The research of microscopy technology combined with high resolution chromosome conformation capture (Hi-C) indicates that euchromatin localized in the nuclear interior and heterochromatin is primarily positioned near the nuclear periphery, whereas the phase separation induced by interactions of the heterochromatin play central roles in establishing compartmentalization [10]. Recent evidence shows that transcription factors (taken Gcn4 as an example) can recruit target genes and tether them to NPC for controlling the spatial arrangement of the yeast genome [11]. Moreover, NPC can regulate the chromatin condensation state by interacting with Poly-comb Repressive Complex, nucleosome, and histone-modifying enzymes [7]. Therefore, future works may focus on the function of NPC in controlling gene activation and spatial organization in plant cells independent traffic roles [12].
Previous studies of NPC mostly focus on the structure and function of metazoan cell or yeast. However, NPCs were poorly understood before the application of advanced biology approaches, such as bioinformatics, atomic force microscopy (AFM), crystallography [13,14]. In the past, the forms of different NPCs were considered to be identical, but several recent studies have uncovered that the expression of NPC components varies in different cell types and different nucleoporins can play precise roles in the development and homeostasis of specific organs [15]. Cryo-EM combined with 3D modeling technology not only uncover novel perspectives in NPC architecture, also reveal that distinct NPC forms (at least three) co-existing in the same cell [16,17]; these distinct forms may be assembled to respond to a particular biological signal.
Our understanding of plant NPC is still far from complete. In our review, we summarize the recent studies of the microstructure and biological functions of plant NPC, and emphasize the biological functions of NPC in the immune defense, growth and development, hormone signaling, and temperature response in plants. We also review the recent findings for regulatory mechanisms by which NPC is involved in plant defensive signaling processes, and compare regulation mechanisms between plants and other organisms.

2. Sketch of Plant NPC

When investigating the functions of the NPC components participating in defensive signaling pathways in plants, the structural composition of plant NPC should be outlined first. Due to their small size and structural plasticity, identifying the microstructures of plant NPC has been a particularly difficult challenge [18]. Continuous advances in research technologies, such as proteomics, crystallography, bioinformatics, and cryo-electron tomography (cryo-ET) [19] have aroused gradually distinct understandings of NPC microstructures in plants [20,21,22,23]. Recently, scientists use a buffer with a high sucrose concentration to purify nuclei and then conduct solubility-based fractionation to increase proteome coverage; finally, they identify two novel nucleoporins (At1g07970 and At3g08870). Interestingly, At3g08879 was first reported as a kinase domain containing protein localized on the NE in plants [24]. However, our understanding of NPC microstructures in plant and their exact components remains incomplete.
In general, NPC has a diameter of 100–150 nm and a depth of 50–70 nm, and is composed of a ring that consists of eight units arranged in radial symmetry [25]. The NPC comprises several conserved modules that can be divided into several subcomplexes, such as the core scaffold, cytoplasmic filaments, nuclear basket, membrane ring, and central FG (Phe-Gly) nucleoporins (that contain intrinsically disordered phenylalanine glycine) (Figure 1) [4].

2.1. Core Scaffold

The core scaffold nucleoporins are the most conserved and flexible among the different NPC modules. The significant flexibility of core scaffold nucleoporins may promote their interaction with specific assemblies such as silencing factor Sir4, histone deacetylase HDAC4 and heterochromatin [26,27,28,29].The function and structure of the core scaffold has been widely studied in yeasts [30], thermophilic fungi [31] and toad [32]. However, the core scaffold remains to be understood in plants. The core scaffold consists of two subcomplexes: the outer ring complex (ORC) and the inner ring complex (IRC).
The ORC is formed by connecting the head to the tail in Y-shaped complexes, which are assembled by 6–10 highly conserved NUPs (the specific number depends on the species), including NUP160, NUP 107, NUP96, NUP133, NUP85, NUP43, NUP37, SEH1, and SEC13 [4]. Eight Y-shaped complexes assemble a ring with an outer diameter of around 125 nm and inner diameter of around 50 nm by connecting the head and tail [32,33]. However, the copies of Y-complex in each ORC are not consistent from species to species. A study describes that the Y-complex of fission yeast is split into two pieces and asymmetrically located at the nuclear and cytoplasmic respective. This asymmetric form of Y-complex is necessary for cell-cycle progression in yeast [34]. Another recent study found that the core scaffold of the early branching eukaryote Chlamydomonas reinhardtii (algal) exhibits differences between human and yeast core scaffolds [35]. First, the algal IRC diameter is 64 nm, and the human IRC diameter is 43 nm, which can facilitate larger molecule trafficking across the algal NPC. Second, the algal ORC is in an asymmetric ‘hybrid’ state, with the nucleus side in a bi-layered ring structure assembled by 16 Y-complexes, with the cytoplasmic side being single-layered (8 Y-complexes). This unique configuration has never been observed in other species, whereas the yeast ORC is in a single-layered state and human ORC is double layered. This asymmetric hybrid configuration indicates an intermediate evolutionary pattern in early branching eukaryotes. This study investigates the variation in the plant nucleocytoplasmic transport mechanism in depth, and identifies the core scaffold as a platform for the substantial evolution and adaptation among different species.
IRC as the fixed point for other NPC frame structures and assembled by NUP205, NUP188, NUP155, NUP135, NUP93, and NUP88, is sandwiched by two ORCs [4]. The IRC with two identical rings are located on the equatorial plane of the NPC equator forming links with membrane rings. Recently, 3D maps obtained by cryo-electron tomography have shown a large radial dilation of the IRC in budding yeast (Saccharomyces cerevisiae), coupled with a larger central channel and an expanded nuclear pore [16]. Therefore, ORC and IRC act as a scaffold for fixing the core structure of NPC, which is pivotal to NPC integrity [30,36,37].

2.2. Cytoplasmic Filaments and Nuclear Baskets

Two characteristic and typical filamentous structures, namely cytoplasmic filaments and nuclear baskets, are asymmetrically distributed on the surfaces of the NPC. These structures are of great significance for specific interactions, serving as docking hubs when transporting biological cargos. The cytoplasmic filaments, which are elongated and structurally disordered polypeptides, have important functions in NPC-capturing cargos. They are mainly composed of NUP214, LOS4, Gle1, CG1, and Rae1 [4]. Moreover, the cytoplasmic filaments play an important role in facilitating the hydrolysis of RanGTP and seem to exclude ribosomes from the space immediately surrounding the nuclear pore [38].
The nuclear basket is constructed of eight protein filaments that are emitted from the NPC core scaffold and join a distal ring into the nucleocytoplasmic side [20]. Its members include NUA, NUP1, NUP50, and NUP82 [4]. Each nuclear basket complex consists of one core architectural and organizational component (in plants this is NUA) which contains a coiled-coil structural domain, and two FG NUPs (NUP136 and NUP50), which bind to the nuclear coaxial ring to connect the NPC core scaffold [39]. Using the proximity labelling-coupled label-free quantitative mass spectrometry (LFQMS) method with NUP82 as bait, a series of nucleoskeleton and nuclear basket were identified. This finding shows that the nuclear basket proteins and the nucleoskeleton are closely interrelated in space [40]. However, most NUPs have no ability to bind directly to DNA or chromatin [41], suggesting that the nucleoskeleton may play a bridge role in nuclear basket involved in regulating gene transcription. With the exception of gene-expression regulation, the NUPs of the nuclear baskets also participate in microRNA biogenesis and transports. For example, NUP1 interacts and colocalizes with THP1 (the core subunits of TREX-2) on the NE to stimulate the export of miRNAs and ARGONAUTE1 (AGO1) from the nucleus to the cytoplasm [42].

2.3. Membrane Ring

The membrane ring is the least conserved of all NPC components and displays considerable flexibility among different species; it includes 3–4 transmembrane nucleoporins. The membrane ring anchors the core components of NPC to the pore membrane via a direct interaction with the core scaffold. The main component of the membrane ring in yeast is Pom152 [43]. Glycoprotein 210 acts as the largest component of membrane ring in vertebrate and is homologous with the yeast Pom152, and is involved in regulating cell differentiation [44]. To date, there are only two conserved membrane ring components that have been found in vertebrates, yeast, and plants, namely Nuclear Division Cycle 1 (NDC1) and Glycoprotein 210 (GP210) [25]. Recently, CONSTITUTIVE EXPRESSION OF PATHOGENESIS-RELATED GENE 5 (CPR5) is identified as a new plant transmembrane nucleoporin and is related to disease resistance in Arabidopsis [45].

2.4. Central FG Nucleoporin

FG nucleoporins account for one-third of all NUPs and are highly conserved among plants and animals. The central FG nucleoporins anchor to the NPC via linker nucleoporins to extend to the cytoplasmic and nuclear sides, and they constitute a central selective channel [46]. The central selective channel acts as gatekeeper to regulate the transport of macromolecules. At present, there are two main hypotheses regarding the selective barrier regulating the transport of macromolecules, namely “selective phase” model and “brush” model [47].
In plants, central FG nucleoporins contain NUP98 and a heterotrimer complex (NUP54-NUP58-NUP62). High speed atomic force microscopy (HS-AFM) results show that they form a liquid–liquid phase separated (LLPS) state to sort cargo transport [48,49], and they use their FG domains to interact with cargo-binding karyopherins that mediate the selective transport of biological cargos larger than around 5 nm or 40 kDa [50]. Selective transport across the central selective channel requires the assistance of nuclear transport receptors (NTRs) [51]. Their efficient transport relies on hydrophobic and electrostatic interactions between FG nucleoporins and NTRs [52]. NTRs can be divided into two groups, importins and exportins. Importins are grouped into importin-α (IMP-α) and importin-β (IMP-β). IMP-α identifies the nuclear localization signal (NLS), and interacts with IMP-β by the B-Binding (IBB) domain for the formation of a heterotrimer (cargo/IMP-α/IMP-β). Subsequently, the heterotrimer complex interacts with central FG nucleoporins to shuttle across the central selective channel. In addition, exportins play a reverse role with importins, which can recognize nuclear export signals (NESs) to facilitate macromolecule export [53]. Among the NTRs identified in human cells, 10 of them are importins, five of them are exportins, three of them are biportins [54]. However, there are still many that are as of yet unknown in kingdom of plants, and the dynamics of central FG nucleoporins are still largely unknown, as central FG nucleoporins LLPS have evaded structural characterization [55].

3. Defensive Roles of NPC in Plants

Different NUP mutants represent distinct phenotypes, and different NUPs are found to be involved in the regulation of distinct plant physiological processes, including defensive responses [56,57,58]. These phenomena imply that in addition to nucleocytoplasmic transport, nucleoporins may have other unexpected functions. Meanwhile, there is increasing evidence that NPC contributes to the spatial structure of chromatin, genome integrity, and gene expression pattern, all of which are carefully controlled by different NUPs, independently of their basic transport functions [59]. It has been shown that the different components of NPC are conducive to regulating distinct physiological processes, including main four aspects: immune defense, growth and development, hormone signaling, and temperature response (summarized in Figure 1 and Table 1).

3.1. Immune Defense

To antagonize pathogens, plants have evolved a set of immune response strategies. Of these, it is important to regulate nucleocytoplasmic bidirectional transport in immune signal molecules [25]. Using forward genetic screening, a series of nucleoporins participating in immune defense and programmed cell death (PCD) are identified and characterized [60]. For instance, BRI1 Associated Kinase 1 (BAK1) is the co-receptor of Flagellin Sensitive 2 (FLS2) and EF-Tu Receptor (EFR). Mutations in nucleoporins such as nup85, nup96, nup160 and seh1 can suppress bak1 bkk1 double-mutant induced PCD [61]. Consistent with this result, NUP96, NUP160 and Seh1 participate in basal immune defense against the bacterial pathogen Pseudomonas syringae. Coincidentally, these three NUPs all belong to ORC and mediate mRNA export from the nucleus, suggesting a sophisticated relationship between nucleocytoplasmic transport and immune response [57].
Another NTR, KA120 (IMP-β family), is involved in immune responses by regulating nucleocytoplasmic distribution. The mutation in KA120 displays resistance to powdery mildew pathogen; in contrast, the overexpression of KA120 efficiently suppresses the autoimmune phenotype of snc1-1. Furthermore, KA120 alters the nucleus distribution and protein activity of SNC1 [62]. In the IMP-α family, IMP-α3 plays a role in defense against P. syringae. IMP-α3 is the main receptor of SNC1 among the nine members of the IMP-α family, and IMP-α3 directly interacts with SNC1 at the protein level both in vivo and in vitro [63]. Beyond bacterial and fungal immune defense, NTRs also participate in virus defense. The virulence factor of cucumber mosaic virus (CMV) 2b protein could bind to the host’s AGOs to weaken the immune response. The phosphorylated state of S40, S42, S28 affects 2b and is recognized by IMP-α for importing into the nucleus and then regulating 2b’s nuclear-cytoplasm distribution [64]. Different NTRs combine distinct molecular cargos and play different roles in response to multiple pathogens and dynamic environmental stimuli. However, the precise regulation mechanisms need to be further explored.
The circadian clock modulates the immune response in plants [65]. The mutation in core scaffold nucleoporin IRC NUP205 results in both a defect in the circadian clock and defective immune responses and is accompanied by changes in the nuclear accumulation level of mRNAs related to circadian rhythm and immune defense. Bacterial infection attenuates the expression of clock genes, as in nup205. This research indicates that after bacterial infection, transcriptional reprogramming requires integrity and a fully functional NPC, which the circadian clock is at least in part responsible for [65].
Furthermore, CPR5, a novel transmembrane nucleoporin, plays a key inhibitory role in plant ETI. Under normal conditions, CPR5 can trigger homo-oligomerization to restrict the nuclear transport of immune signal cargo; with the NB-LRR receptors activated, CPR5 undergoes a conformation transition from oligomer to monomer, and then enhances NPC permeability, causes an influx of immune-related signaling molecules, and finally stimulates a variety of downstream stress-related signaling pathways [45]. Meanwhile, mutations in NUP54 and NUP58 enhance the autoimmune phenotype in cpr5; in contrast, the autoimmunity of cpr5 could be suppressed by the mutation of nup88 [45] by attenuating the nuclear export of some immune-related proteins, such as EDS1 and NPR1 [66]. Moreover, the loss-of-function of EXPORTIN4 (XPO4) enhances the autoimmunity of cpr5 by counteracting the nuclear accumulation of TOPLESS (TPL) and TPL related (TPR) proteins; XPO4 coordinates the nuclear accumulation of TPL/TPRs, which co-repress the immune negative regulators and are redundantly required for ETI induction [67]. These examples suggest that nuclear protein influx and efflux are regulated by distinct NPC constituents to mount a robust immune response. In addition, CPR5 is a component of the RNA processing complex (Transformer 2 subfamily in the SR superfamily) and coordinates with NTC and CPSF in plant immunity. Furthermore, CPR5 is the first transmembrane nucleoporin identified in the SR superfamily. Meanwhile, data from the cpr5 RNA-sequencing indicate that CPR5 participates in the regulation of alternative splicing (AS). Hence, CPR5 may act as a component of LLPS [68] which is involved in the plant immune response [69]. Collaboratively, NUP54, NUP58, NUP88, and XPO4 cooperate with CPR5 to regulate the disease resistance, highlighting that the integrity of the NPC plays pivotal roles in immune signals.
The HOS1 transcript in peaches (Prunus persica) is upregulated in response to priming resistance to Rhizopus stolonifer due to a physical interaction with PpWRKY22 in vivo [70]. In Lotus japonicas, a defect in GLE1 slows the symbiotic association with rhizobia, whereas the nitrogen fixation capacities of the gle1 nodules are far below the wild type, indicating that GLE1 is critical for the mutualistic symbiosis between legumes and rhizobia [71]. This confirms the significant role of NPC responses to symbiotic and pathogenic microorganisms in leguminous plants.
Salicylic acid plays an absolutely necessary role in plant defense against pathogens. Some NUPs are involved in salicylic acid-dependent immune responses. Single nup82 mutant has no obvious differences from the wild type, but the nup82 nup136 double mutant shows severe growth defects and impaired benzothiadiazole (an analog of salicylic acid) induced resistance to P. syringae [72]. This study shows that there is redundancy in the functions of NUP136 and NUP82. The compromised immune function of the nup82 nup136 double mutant due to the integrity of the nuclear basket is destroyed; and their deletion triggers an immune defect, in the same way as the nua (another nuclear basket protein) mutant, which highlights their collaborative role in maintaining the integrity of NPC and the plant immune response. Furthermore, NUP136 interacts with the Transcription Export 2 (TREX-2) complex and boosts miRNAs and AGO1 export [42], both of which influence the expression patterns of some broad spectrum immune defensive genes [73,74]. The function of three nuclear basket nucleoporins, NUP82, NUP136 and NUA in defensive pathway implies that the nuclear basket is particularly sensitive to the fluctuation of salicylic acid signal.
Pathogen-responsive mitogen-activated protein kinase (MAPK/MPK) cascades are extremely important for activating immune receptors, and then downstream target genes [75]. Furthermore, nup88 is hypersensitive to Botrytis cinerea with reduced activated MPK3 and MPK6 levels. It is possible that the loss-of-function of NUP88 promotes the nuclear export of MPK3 and thus jeopardizes its nuclear accumulation, resulting in compromised resistance to fungi [76]. Furthermore, MPK6 targets the phosphorylated sites VPSSTPLIK(s)*PVATTQQLPK and AP(s)*PGGGSSTIVTLADR of NUA;. The diminished single and dual phosphorylation of SPEKEEVQPETLA(t)*PTQ(s)*PSR of NUA in mpk3 and mpk6 mutants [77] reduce resistance to the bacterial pathogen P. syringae [25]. Nevertheless, the dependence of this phenomenon on the phosphorylation site of MPK6 requires further evidence.
Another central FG nucleoporin, NUP98, resists necrotrophic fungal pathogen in an NUP88-dependent manner. Indeed, the nup98ab double mutant is more susceptible to B. cinerea infection [76], while NUP88 interacts with NUP98A and NUP98B to affect the permeability of NPC for the transportation of some immune-related cargos. As with rice, the knockdown of APIP12 (AvrPiz-t interacting protein 12, homolog of AtNUP98) causes an enhanced susceptibility to Magnaporthe oryzae by suppressing the expression of pathogenesis related (PR) genes [78]. It is worth noting that NUP54, NUP58, and NUP98, all belong to the central selective barrier, but exhibit opposite immune responses to different fungal pathogen, indicating that different FG-NUPs are distinct in framing the selective channel and in sorting biological cargos [2]. The opposite phenotype caused by a different component of NPC may imply that the two of them feature entirely different mechanisms in disease resistance.

3.2. Plant Growth and Development

Early flowering is used as a drought-escape mechanism and late flowering is a cold-response mechanism in plants. Previous studies have shown that NUPs function in the regulation of flowering, implying that NUPs may act against harsh environments by changing flowering time. Some nup mutants, such as nup160, nup96, hos1, and nup98, show early flowering in Arabidopsis. NUP160 and NUP96 stabilize HOS1 protein, a negative regulator of cold, to negatively regulate flower time via ubiquitination and subsequent 26s proteasome degradation of CONSTANT (CO). Coincidentally, NUP160, NUP96 and HOS1 all belong to the ORC component, and function in the regulation of flowering through controlling CO protein level [79,80].
However, with the exception flowering time relying on CO, two redundant nucleoporins NUP98A and NUP98B bypass the CO checkpoint in photoperiodic signaling and integrated signals from many other pathways to take FT as a direct target for flowering control [81]. The nup98ab double mutant accumulates more starch and demonstrates a much earlier senescence phenotype compared to control plants, indicating that abnormalities in starch degradation and energy metabolism are the main cause of earlier senescence in nup98ab [82]. In addition to participating in the control of flowering, NUP98 positively regulates hypocotyl elongation in response to shade avoidance syndrome (SAS), by modulating shade-induced gene expression in a transport independent manner [83].
In Arabidopsis, NUA negatively mediates flowering under both short and long day conditions. NUA recruits and interacts with EARLY IN SHORT DAYS 4 (ESD4), a SUMO protease, which is located at the nuclear rim, to change the accumulation of SUMO conjugates to control flowering [84]. This indicates that NUA functions not only in mRNA export, but also sumoylation [84]. In human, biallelic variants in TPR (NUA in plants) cause microcephaly, ataxia and severe intellectual disability, accompanied by a decrease in nucleus global RNA intensity [85]. This cognition regarding the nuclear basket shows that beyond providing anchors for mRNA transport, it has another mechanism involved in regulating transcription. TPR directly interacts with GANP, a component of the TREX-2 complex [86], demonstrating that TREX-2 complex associate tightly with the NPC in a TPR dependent manner. Furthermore, the maintenance of memory gene loops (MGLs) could lead genes to re-induce quickly and facilitate mRNA accumulation during short term periods of transcriptional repression. Myosin-like protein 1 (Mlp1), a homolog gene of plant NUA in yeast, is important for assembling and maintaining the MGLs. Furthermore. Mlp1 tethers MGLs to the NPC by interacting with MGLs. These two completely different regulatory mechanisms of NUA provide new views for the regulatory mechanism of NUA in plants [87,88].
The nup160, nup96 and nua mutants all exhibit early flowering and accompany nuclear mRNA accumulation; nup85 and seh1 also exhibit nucleus accumulation of mRNA, however, with no significant flowering phenotype [25]. Therefore, distinct NUPs may be responsible for transporting entirely different sub-pools of mRNAs, or may have other functions independent to mRNA transport, thus resulting in particular phenotypes. Moreover, disturbing the function of plant NUPs causes a significant developmental defect phenotype. The hos1 mutant exhibits a longer hypocotyl phenotype in light conditions but a shorter one in dark conditions relative to control, similar as in auxin overproducing mutants [89]. Additionally, HOS1 can directly interact with PHYTOCHROME INTERACTING FACTOR4 (PIF4) to participate in phytochrome B (phyB)-mediated hypocotyl elongation [90,91]. The light signal mediated by phytochrome induces the recruitment of light-related genes to the nuclear periphery during transcriptional activation. The heterologous expression of AtNUP62 in tobacco leads to leaf tissue damage, indicating that the function of AtNUP62 is to maintain tissue integrity [92].
The gametogenesis of male and female is a complex biological process that requires a coordinated expression of genes to regulate the cell proliferation and differentiation. NPC are essential cellular structures that assemble during cell proliferation and differentiation, and transport extensive material cargos for this process. Thus, the mutants of several NPC components have been found to affect embryonic development. Arabidopsis NUP1 participate in pollen and ovule development. The nup1 mutant causes defects in both male gametogenesis and female gametogenesis fertility. Most ovules of nup1-4 stagnate during meiosis, and approximately only 3% of ovules develop to the FG7 stage; the development of pollen grains is aborted during the first mitotic division. Relative to the wild type, the expression of genes related to megasporogenesis and pollen development are significantly down regulated in nup1-4 mutant [93]. The restriction of enzyme-mediated chromatin immunoprecipitation (RE-ChIP) assays, uses NUP1-GFP as bait and NUP1 is primarily associated with the transcriptionally repressed chromatin region [94]. It is possible that NUP1/136 directly mediates the gene transcription by recruiting transcriptionally repressed chromatin regions to the NPC [93]. Loss-of-function of NUA also produces defects in gametogenesis by disturbing miRNA and mRNA exports [42,84]. Furthermore, NUP88 participates in gametophyte formation during mitosis in Arabidopsis, and nup88 displays a failure of spindle and microtubule formation, a fate specification of gamete cells, impairs ovule and pollen development, and eventually leads to seed abortion [95]. In addition to NUP1, NUA and NUP88, LNO1 is also required for plant embryogenesis and seed viability. As is known, LNO1 is a FG-nucleoporin that is homologous with human NUP214 and yeast NUP159. Homozygous lno1 is lethal, and the seed viability of heterozygous mutant is aborted. The deletion of LNO1 abolishes asymmetrical cell division during the first zygotic formation, and alters the planes and number of cell divisions in early embryogenesis, which eventually leads to abnormal embryogenesis and embryo abortion [96]. Whether these functions are modulated by specific cell signaling transductions in light of macromolecular transport or transport-independent functions is a challenge to be addressed in the near future.

3.3. Plant Hormone Signaling

As reported, some phytohormone signaling pathways feature unique sensitivity to disturbance in plant NPC. Auxin is the main naturally occurring phytohormone that coordinates and steers cell growth and organ differentiation. Furthermore, NUP160, NUP96, and NUA participate in the auxin response, because corresponding nucleoporin mutants are screened as suppressors of the auxin-resistant1 (axr1) mutant [97]. Additionally, NUP62 also plays an important role in the auxin response. The nup62-1 mutant displays small cotyledons, reduced leaf blades, malformed siliques, and earlier bolting than wild type, while nup62 mutants are hypersensitive to auxin at the root and cotyledon levels. Furthermore, a mutation in NUP62 leads to an increased activity in the DR5 synthetic promoter. This suggests that NUP62 is a major negative regulator that participates in the auxin signaling response or transportation [97]. Loss-of-function of TRANSCURVATA 1 (AtTCU1, the homologous gene of NUP58) shows pleiotropic phenotypes, such as hypocotyl elongation, reduced vegetative leaf size accompanied by a reticulated and greener vein, and early flowering through a direct interaction with auxin signaling pathways [98]. Furthermore, HOS1 negatively regulates auxin biosynthesis in Arabidopsis hypocotyl elongation control [89]. In the apple, MdNUP54 and MdNUP62 also respond to auxin treatment. Interestingly, 3-Indoleacetic acid (IAA) significantly restrains the expression of seven NUP genes and delays apple flowering.
Nucleoporins also participate in the abscisic acid (ABA) signal response. The nup85 is identified by a forward genetic screen, which causes RESPONSIVE TO DESICCATION 29A (RD29A) to reduced expression in response to ABA. After 3 h treatment with 50-µM ABA, several stress related genes including RD29A, COLD REGULATED 15A (COR15A) and COR47 are obviously downregulated in nup85. That study demonstrates that nup160 and hos1 are sensitive to exogenous ABA as well. Strikingly, a direct internal interaction between NUP85 and Mediator 18 (MED18) is identified by immunoprecipitation combined with mass spectrometry. A direct interaction between NUP85 and MED18 connects the association between NPC components and Mediator core subunits, and suggests the possibility of NUP85 or other NPC components associated with Mediator complex that directly link the regulation of downstream gene transcription mediated by RNA Pol II. However, there is no evidence indicating the member of the NUP107-160 complex directly interacts with and tethers chromatin regions to regulate genes involved in phytohormone signaling [99]. Indeed, auxin and ABA are considered as the two most significantly affected hormones based on cpr5 transcriptome data [45]. Research on the biological significance of the crosstalk between NPC and plant hormone signaling pathways may yield novel insights into potential regulatory mechanisms.
Furthermore, CPR5 participates in the ethylene signal. It directly interacts with the N-terminal transmembrane domains of ETHYLENE RESPONSE 1 (ETR1), which is the major ethylene receptor in Arabidopsis. It can selectively regulate the nucleocytoplasmic transport of mRNAs related to ethylene signaling [100]. Regarding the relationship between NPC and the ethylene signal, there is another classic model that is involved in the tethering of ETHYLENE INSENSITIVE2 (EIN2) to regulate its nucleocytoplasmic transport. Ethylene triggers EIN2 dephosphorylation, cleavages its C-terminal cytosolic region from transmembrane domain, and frees the C-terminal to enter the nucleus to trigger EIN3 and EIL1 expression [101,102,103]. It remains to be clarified whether NPC plays a role in this classic model and which component of NPC mediates this process.

3.4. Temperature Response

Ambient temperature has a significant influence on plant growth [104], organ development [105] and abiotic or biotic stress responses [106]. Previous studies have indicated that the plant NPC plays important roles in response to temperature cues. The observation of temperature-sensitive defects of some nucleoporin mutants has encouraged more detailed explorations of the role of NPC in perceiving ambient temperature change and in the assessments of how to test speculated mechanisms.
The NUPs of ORC are most frequently reported in the temperature response. Furthermore, NUP160 plays a crucial role in the cold response by impairing the level of cold induced transcription factor CBF3. The nup160-1 mutant is more sensitive to chilling and features an altered expression of cold relevant genes by impacting the accumulation of mRNA in the nucleus [107]. Furthermore, the nup96, nup160, and hos1 mutants all elongate the hypocotyl to respond to high temperatures by suppressing the nuclear accumulation of PHYTOCHROME INTERACTING FACTOR 4 (PIF4), a key factor involved in perceiving temperature and photosynthetic signals. Moreover, the nup133 and nup96 mutants show defects in fresh weight after temperature change [108]. As an attenuator of cold stress signaling, HOS1 reduces the expression of cold responsive gene to decrease cold tolerance [109]. Additionally, HOS1, which functions as a RING E3 ubiquitin ligase, directly interacts with Inducer of Cold Expression 1 (ICE1) to mediate the ubiquitination and degradation of ICE1 [110]. Importantly, it responds to cold stress by changing the synthesis of secondary metabolites such as glucosinolates (GSLs), phytoalexins, and flavonol glycosides [111]. These results underscore that different members of ORC may play different roles in responding to different temperatures, indicating the existence of temperature influences on ORC complex integrity and activity [108].
Whether HOS1 is a component of NPC and whether it has nuclear localization are still controversial [112]. The GFP-HOS1 fluorescence signal is detectable in both the nuclear periphery and nucleoplasm, which implies that HOS1 may not be constitutively tethered by NPC [79]. Meanwhile, other proteins such as OPEN STOMATA 1(OST1) [113], BR INSENSITIVE 2 (BIN2) [105] affect the interaction between ICE1 and HOS1 to disturb the ubiquitination and degradation of ICE1 in cold resistance. In addition to its function as an E3 ubiquitin ligase, HOS1 associates with chromatin to regulate gene transcription via its non-proteolytic function [114,115], or binds to NPC to regulate mRNA export [116]. It induces thermo-tolerance by activating DNA repair components in a heat shock protein 90 (HSP90)-dependent manner [117]. In the apple, the expression of MdNUP62 is significantly upregulated after heat treatments in cultured seedlings of ‘Nagafu No. 2’, transgenic lines that heterologously overexpress MdNUP62 in Arabidopsis and tomato show a significantly reduced tolerance to high temperature [118].
Table
Table 1. Summary physiological processes involved in plant NPC.
Table 1. Summary physiological processes involved in plant NPC.
Related ProcessGeneLocationOrganismal RoleCellular RoleReference
Immune defenseNUP96ORCBasal immune defense against P. syringaeInfluence nuclear mRNA export[57,60,61]
NUP160ORCBasal immune defense against P. syringaeInfluences nuclear mRNA export and protein level of EDS1[57,60,61]
Seh1ORCBasal immune defense against P. syringaeInfluences nuclear mRNA export and protein level of EDS1[57,60,61]
NUP85ORCBasal immune defense against P. syringaeInfluences nuclear mRNA export and involved in endogenous salicylic acid accumulation[60,61]
HOS1ORCRelated to the resistance to R. stoloniferaInteracts with PpWRKY22 in vivo[70]
NUP205IRCRelated to circadian rhythm and immune defenseInfluences the nuclear accumulation level of mRNAs related to circadian rhythm and immune defense[65]
KA120IMP-β familyRelated to the resistance to powdery mildew pathogenAlters the nucleus distribution and protein activity of SNC1[62]
IMP-α3IMP-α familyBasal immune defense against P. syringaeInteracts with SNC1 at the protein level both in vivo and in vitro[63]
IMP-αIMP-α familyInfluences RNA silencing of cucumber mosaic virus 2b proteinMediates nucleocytoplasmic shuttling of 2b protein[64]
XPO4exportinsParticipates in the regulation of CPR5 mediated ETICoordinates the nuclear accumulation of TPL/TPRs[67]
CPR5Membrane ringA key inhibitory role in plant ETIParticipates in transporting of immune signal cargos[45]
NUP54Central channelParticipates in the regulation of CPR5 mediated ETIMutation in nup54 enhance the autoimmune phenotype in cpr5[45]
NUP58Central channelParticipates in the regulation of CPR5 mediated ETIMutation in nup58 enhance the autoimmune phenotype in cpr5[45]
NUP98Central channelRelated to the resistance to necrotrophic fungal pathogenInteracts with NUP88 and suppress the expression of pathogenesis related (PR) genes[76,78]
NUP82Nuclear basketRelated to salicylic acid mediated immune response to P. syringaeInteracts with NUP136 and RAE1, causes downregulation of immune related genes[72]
NUP136Nuclear basketRelated to salicylic acid mediated immune response to P. syringaeInteracts with NUP82, causes downregulation of immune related genes[72]
NUANuclear basketRelated to the resistance to P. syringae [25]
NUP88LinkerRelated to CPR5 and MAPK/MPK mediated immune responseInteracts with NUP98, affect the permeability of NPC and transportation of immune-related cargos[45,76]
GLE1Cytoplasmic flamentsPlays a distinct role in the symbiotic association between legumes and rhizobiaFunctions in the export of mRNAs from the nucleus into the cytoplasm[71]
Plant growth and developmentNUP96ORCRegulates flowering time and hypocotyl elongationInteracts with HOS1, affects protein level of CO, critical for nuclear localization of PIF4 protein[80]
NUP160ORCRegulates flowering time and hypocotyl elongationInteracts with HOS1, affects protein level of CO, critical for nuclear localization of PIF4 protein[79]
NUP98ORCInhibit flowering through clock, photoperiod, and age pathways, regulates hypocotyl elongationInduces FT expression in a CO independent manner, modulates shade induced genes expression[81,82,83]
HOS1ORCRegulates flowering time and hypocotyl elongationAffects protein level of CO, interacts with PIF4 and is critical for nuclear localization of PIF4 protein[79,80,81,82,83,84,85,86,87,88,89,90,91,112]
NUANuclear basketMediates the flowering under both SD and LD conditions, affects the male and female gametogenesisInteracts with ESD4, MAD1 and MAD2, affects formation of kinetochores during prophase and prometaphase[84]
NUP1Nuclear basketParticipates in pollen and ovule developmentRecruits transcriptionally repressed chromatin regions to the NPC[93,94]
NUP62Central channelMediates the growth and development of leavesControls of nuclear transport and maintaining tissue integrity[92]
NUP88LinkerAffects the development of ovule and pollen, and eventually leads to seed abortionAffects spindle and microtubule formation, gamete cells fate specification[95]
LONO1Cytoplasmic flamentsMediates abnormal embryogenesis and embryo abortionAbolishes asymmetrical cell division during the first zygotic formation[96]
hormone signalingNUP96ORCAuxin responseinfluences nuclear mRNA export, affects the localization of AXR3[97]
NUP160ORCABA and auxin responseInfluences nuclear mRNA export, affects the localization of AXR3[97,99]
HOS1ORCABA and auxin responseRegulates expressions of YUCs, PINs, CTR1, CYP79B2[99]
NUP85ORCABA responseInteracts with MED18 and regulates expressions of RD29A, COR15A, COR47[99]
NUANuclear basketAuxin responseInfluence nuclear mRNA and miRNA export[97]
NUP62Central channelAuxin responseInfluences the activity of the DR5 auxin responsive promoter[97]
NUP58Central channelAuxin responseInteracts with SCF (Skp1/Cul1/F-box) ubiquitin ligase complex components[98]
CPR5Membrane ringABA and auxin response and ethylene signalInteracts with ETR1, regulates the nucleocytoplasmic transport of mRNAs related to ABA, auxin and ethylene signaling[45,100]
Temperature responseNUP160ORCCold and high temperature responseInfluence nuclear mRNA export[107,108]
NUP96ORCHigh temperature responseAffect the nuclear localization of PIF4 at elevated temperature[108]
HOS1ORCHigh temperature responseAffect the nuclear localization of PIF4 at elevated temperature[108,117]
NUP133ORCHigh temperature responseImpacts nuclear accumulation of the IAA17 protein at elevated temperature[108]
NUP62Central channelHigh temperature responseInteracts with multiple MdHSFs[118]

4. Future Perspective

Plant NPC is considered as an important hub that regulates plant defensive processes. Following the investigation of the phenotype for NUP mutants and the analysis of plant NPC proteome data, significant progress has been made in studies of the NPC structure and biological functions. Some questions on NPCs still require more attention in future studies. (1) Are there other undiscovered proteins as components of NPC? What is the microstructure of plant NPC? The traditional understanding of plant NPC micro-structure is based on insights from yeast and vertebrates. With the progress of microscope technology, it is likely that the plant NPC micro-structure will be resolved in the near future. (2) Can plant NUPs directly bind to chromatin to control gene expression for defense response like animals? What are the effects of the particular spatial location of chromatin to the NPC on gene transcription and epigenetic regulation under harsh conditions? (3) Plant ontogenetic defense against abiotic and biotic stresses has been extensively studied [119,120,121]. Studies have indicated that NPCs functions in plant developmental transitions. Are NPCs involved in the plant ontogenetic defensive pathway? How do NPCs interact with age factors? Additionally, what dynamic changes occur in NPC with plant age or environmental changes? Clarifying the answers to these questions will produce novel insights into the potential regulatory mechanisms of NPCs in plants for agricultural progress and relieve the effects of growing stress factors due to global environmental change.

Author Contributions

X.W., J.H. and C.G. conceived the manuscript. X.W. wrote the manuscript; C.G. revised the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by National Natural Science Foundation of China (31770352), and Natural Science Foundation of Zhejiang Province (LY20C020002).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We thank Gang Wu (Zhejiang A & F University) for useful comments on this manuscript. We thank the anonymous reviewers for the constructive and positive comments.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Alber, F.; Dokudovskaya, S.; Veenhoff, L.M.; Zhang, W.; Kipper, J.; Devos, D.; Suprapto, A.; Karni-Schmidt, O.; Williams, R.; Chait, B.T.; et al. Determining the architectures of macromolecular assemblies. Nature 2007, 450, 683–694. [Google Scholar] [CrossRef] [PubMed]
  2. Lüdke, D.; Rohmann, P.F.W.; Wiermer, M. Nucleocytoplasmic communication in healthy and diseased plant tissues. Front. Plant Sci. 2021, 12, 719453. [Google Scholar] [CrossRef] [PubMed]
  3. Wälde, S.; Kehlenbach, R.H. The part and the whole: Functions of nucleoporins in nucleocytoplasmic transport. Trends Cell Biol. 2010, 20, 461–469. [Google Scholar] [CrossRef]
  4. Meier, I.; Richards, E.J.; Evans, D.E. Cell biology of the plant nucleus. Annu. Rev. Plant Biol. 2017, 68, 139–172. [Google Scholar] [CrossRef]
  5. Groves, N.R.; Biel, A. Recent advances in understanding the biological roles of the plant nuclear envelope. Nucleus 2020, 11, 330–346. [Google Scholar] [CrossRef] [PubMed]
  6. D’Angelo, M.A. Nuclear pore complexes as hubs for gene regulation. Nucleus 2018, 9, 142–148. [Google Scholar] [CrossRef] [Green Version]
  7. Kuhn, T.M.; Capelson, M. Nuclear pore proteins in regulation of chromatin state. Cells 2019, 8, 1414. [Google Scholar] [CrossRef] [Green Version]
  8. Misteli, T. The self-organizing genome: Principles of genome architecture and function. Cell 2020, 183, 28–45. [Google Scholar] [CrossRef]
  9. Sumner, M.C.; Brickner, J. The nuclear pore complex as a transcription regulator. Cold Spring Harb. Perspect. Biol. 2022, 14, a039438. [Google Scholar] [CrossRef]
  10. Falk, M.; Feodorova, Y.; Naumova, N.; Imakaev, M.; Lajoie, B.R.; Leonhardt, H.; Joffe, B.; Dekker, J.; Fudenberg, G.; Solovei, I.; et al. Heterochromatin drives compartmentalization of inverted and conventional nuclei. Nature 2019, 570, 395–399. [Google Scholar] [CrossRef] [PubMed]
  11. Brickner, D.G.; Randise-Hinchliff, C.; Lebrun Corbin, M.; Liang, J.M.; Kim, S.; Sump, B.; D’Urso, A.; Kim, S.H.; Satomura, A.; Schmit, H.; et al. The role of transcription factors and nuclear pore proteins in controlling the spatial organization of the yeast genome. Dev. Cell 2019, 49, 936–947.e4. [Google Scholar] [CrossRef]
  12. Tamura, K. Nuclear pore complex-mediated gene expression in Arabidopsis thaliana. J. Plant Res. 2020, 133, 449–455. [Google Scholar] [CrossRef]
  13. Mohamed, M.S.; Kobayashi, A.; Taoka, A.; Watanabe-Nakayama, T.; Kikuchi, Y.; Hazawa, M.; Minamoto, T.; Fukumori, Y.; Kodera, N.; Uchihashi, T.; et al. High-speed atomic force microscopy reveals loss of nuclear pore resilience as a dying code in colorectal cancer cells. ACS Nano 2017, 11, 5567–5578. [Google Scholar] [CrossRef]
  14. Stanley, G.J.; Akpinar, B.; Shen, Q.; Fisher, P.D.E.; Lusk, C.P.; Lin, C.; Hoogenboom, B.W. Quantification of biomolecular dynamics inside real and synthetic nuclear pore complexes using time-resolved atomic force microscopy. ACS Nano 2019, 13, 7949–7956. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Guglielmi, V.; Sakuma, S.; D’Angelo, M.A. Nuclear pore complexes in development and tissue homeostasis. Development 2020, 147. [Google Scholar] [CrossRef]
  16. Akey, C.W.; Singh, D.; Ouch, C.; Echeverria, I.; Nudelman, I.; Varberg, J.M.; Yu, Z.; Fang, F.; Shi, Y.; Wang, J.; et al. Comprehensive structure and functional adaptations of the yeast nuclear pore complex. Cell 2022, 185, 361–378.e5. [Google Scholar] [CrossRef]
  17. Fernandez-Martinez, J.; Rout, M.P. One ring to rule them all? Structural and functional diversity in the nuclear pore complex. Trends Biochem. Sci. 2021, 46, 595–607. [Google Scholar] [CrossRef]
  18. Hayama, R.; Rout, M.P.; Fernandez-Martinez, J. The nuclear pore complex core scaffold and permeability barrier: Variations of a common theme. Curr. Opin. Cell Biol. 2017, 46, 110–118. [Google Scholar] [CrossRef]
  19. Zhang, Y.; Li, S.; Zeng, C.; Huang, G.; Zhu, X.; Wang, Q.; Wang, K.; Zhou, Q.; Yan, C.; Zhang, W.; et al. Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex. Cell Res. 2020, 30, 532–540. [Google Scholar] [CrossRef]
  20. Strambio-De-Castillia, C.; Niepel, M.; Rout, M.P. The nuclear pore complex: Bridging nuclear transport and gene regulation. Nat. Rev. Mol. Cell. Biol. 2010, 11, 490–501. [Google Scholar] [CrossRef]
  21. Tamura, K.; Fukao, Y.; Iwamoto, M.; Haraguchi, T.; Hara-Nishimura, I. Identification and characterization of nuclear pore complex components in Arabidopsis thaliana. Plant Cell 2010, 22, 4084–4097. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Beck, M.; Hurt, E. The nuclear pore complex: Understanding its function through structural insight. Nat. Rev. Mol. Cell. Biol. 2017, 18, 73–89. [Google Scholar] [CrossRef] [PubMed]
  23. Lin, D.H.; Hoelz, A. The structure of the nuclear pore complex (an update). Annu. Rev. Biochem. 2019, 88, 725–783. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Goto, C.; Hashizume, S.; Fukao, Y.; Hara-Nishimura, I.; Tamura, K. Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction. Nucleus 2019, 10, 81–92. [Google Scholar] [CrossRef] [Green Version]
  25. Li, X.; Gu, Y. Structural and functional insight into the nuclear pore complex and nuclear transport receptors in plant stress signaling. Curr. Opin. Plant Biol. 2020, 58, 60–68. [Google Scholar] [CrossRef]
  26. Van de Vosse, D.W.; Wan, Y.; Lapetina, D.L.; Chen, W.M.; Chiang, J.H.; Aitchison, J.D.; Wozniak, R.W. A role for the nucleoporin Nup170p in chromatin structure and gene silencing. Cell 2013, 152, 969–983. [Google Scholar] [CrossRef] [Green Version]
  27. Kehat, I.; Accornero, F.; Aronow, B.J.; Molkentin, J.D. Modulation of chromatin position and gene expression by HDAC4 interaction with nucleoporins. J. Cell Biol. 2011, 193, 21–29. [Google Scholar] [CrossRef] [Green Version]
  28. Gozalo, A.; Duke, A.; Lan, Y.; Pascual-Garcia, P.; Talamas, J.A.; Nguyen, S.C.; Shah, P.P.; Jain, R.; Joyce, E.F.; Capelson, M. Core components of the nuclear pore bind distinct states of chromatin and contribute to polycomb repression. Mol. Cell 2020, 77, 67–81.e7. [Google Scholar] [CrossRef]
  29. Iglesias, N.; Paulo, J.A.; Tatarakis, A.; Wang, X.; Edwards, A.L.; Bhanu, N.V.; Garcia, B.A.; Haas, W.; Gygi, S.P.; Moazed, D. Native chromatin proteomics reveals a role for specific nucleoporins in heterochromatin organization and maintenance. Mol. Cell 2020, 77, 51–66.e8. [Google Scholar] [CrossRef]
  30. Kim, S.J.; Fernandez-Martinez, J.; Nudelman, I.; Shi, Y.; Zhang, W.; Raveh, B.; Herricks, T.; Slaughter, B.D.; Hogan, J.A.; Upla, P.; et al. Integrative structure and functional anatomy of a nuclear pore complex. Nature 2018, 555, 475–482. [Google Scholar] [CrossRef] [Green Version]
  31. Thierbach, K.; von Appen, A.; Thoms, M.; Beck, M.; Flemming, D.; Hurt, E. Protein interfaces of the conserved Nup84 complex from chaetomium thermophilum shown by crosslinking mass spectrometry and electron microscopy. Structure 2013, 21, 1672–1682. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  32. Huang, G.; Zhang, Y.; Zhu, X.; Zeng, C.; Wang, Q.; Zhou, Q.; Tao, Q.; Liu, M.; Lei, J.; Yan, C.; et al. Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis. Cell Res. 2020, 30, 520–531. [Google Scholar] [CrossRef]
  33. Stuwe, T.; Correia, A.R.; Lin, D.H.; Paduch, M.; Lu, V.T.; Kossiakoff, A.A.; Hoelz, A. Nuclear pores architecture of the nuclear pore complex coat. Science 2015, 347, 1148–1152. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  34. Asakawa, H.; Kojidani, T.; Yang, H.J.; Ohtsuki, C.; Osakada, H.; Matsuda, A.; Iwamoto, M.; Chikashige, Y.; Nagao, K.; Obuse, C.; et al. Asymmetrical localization of Nup107-160 subcomplex components within the nuclear pore complex in fission yeast. PLoS Genet. 2019, 15, e1008061. [Google Scholar] [CrossRef] [Green Version]
  35. Mosalaganti, S.; Kosinski, J.; Albert, S.; Schaffer, M.; Strenkert, D.; Salome, P.A.; Merchant, S.S.; Plitzko, J.M.; Baumeister, W.; Engel, B.D.; et al. In situ architecture of the algal nuclear pore complex. Nat. Commun. 2018, 9, 2361. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Lin, D.H.; Stuwe, T.; Schilbach, S.; Rundlet, E.J.; Perriches, T.; Mobbs, G.; Fan, Y.; Thierbach, K.; Huber, F.M.; Collins, L.N.; et al. Architecture of the symmetric core of the nuclear pore. Science 2016, 352, aaf1015. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Fischer, J.; Teimer, R.; Amlacher, S.; Kunze, R.; Hurt, E. Linker Nups connect the nuclear pore complex inner ring with the outer ring and transport channel. Nat. Struct. Mol. Biol. 2015, 22, 774–781. [Google Scholar] [CrossRef]
  38. Fiserova, J.; Spink, M.; Richards, S.A.; Saunter, C.; Goldberg, M.W. Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. J. Cell Sci. 2014, 127, 124–136. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  39. Tamura, K.; Hara-Nishimura, I. The molecular architecture of the plant nuclear pore complex. J. Exp. Bot. 2013, 64, 823–832. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  40. Tang, Y.; Huang, A.; Gu, Y. Global profiling of plant nuclear membrane proteome in Arabidopsis. Nat. Plants 2020, 6, 838–847. [Google Scholar] [CrossRef] [PubMed]
  41. Buchwalter, A.; Kaneshiro, J.M.; Hetzer, M.W. Coaching from the sidelines: The nuclear periphery in genome regulation. Nat. Rev. Genet. 2019, 20, 39–50. [Google Scholar] [CrossRef]
  42. Zhang, B.; You, C.; Zhang, Y.; Zeng, L.; Hu, J.; Zhao, M.; Chen, X. Linking key steps of microRNA biogenesis by TREX-2 and the nuclear pore complex in Arabidopsis. Nat. Plants 2020, 6, 957–969. [Google Scholar] [CrossRef] [PubMed]
  43. Upla, P.; Kim, S.J.; Sampathkumar, P.; Dutta, K.; Cahill, S.M.; Chemmama, I.E.; Williams, R.; Bonanno, J.B.; Rice, W.J.; Stokes, D.L.; et al. Molecular architecture of the major membrane ring component of the nuclear pore complex. Structure 2017, 25, 434–445. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Raices, M.; Bukata, L.; Sakuma, S.; Borlido, J.; Hernandez, L.S.; Hart, D.O.; D’Angelo, M.A. Nuclear pores regulate muscle development and maintenance by assembling a localized Mef2C complex. Dev. Cell 2017, 41, 540–554.e7. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Gu, Y.; Zebell, S.G.; Liang, Z.; Wang, S.; Kang, B.H.; Dong, X. Nuclear pore permeabilization is a convergent signaling event in effector-triggered immunity. Cell 2016, 166, 1526–1538.e11. [Google Scholar] [CrossRef] [Green Version]
  46. Grossman, E.; Medalia, O.; Zwerger, M. Functional architecture of the nuclear pore complex. Annu. Rev. Biophys. 2012, 41, 557–584. [Google Scholar] [CrossRef] [PubMed]
  47. Stewart, M. Function of the nuclear transport machinery in maintaining the distinctive compositions of the nucleus and cytoplasm. Int. J. Mol. Sci. 2022, 23, 2578. [Google Scholar] [CrossRef]
  48. Schmidt, H.B.; Görlich, D. Nup98 FG domains from diverse species spontaneously phase-separate into particles with nuclear pore-like permselectivity. Elife 2015, 4, e04251. [Google Scholar] [CrossRef] [PubMed]
  49. Wong, R.W. New activities of the nuclear pore complexes. Cells 2021, 10, 2123. [Google Scholar] [CrossRef] [PubMed]
  50. Raveh, B.; Karp, J.M.; Sparks, S.; Dutta, K.; Rout, M.P.; Sali, A.; Cowburn, D. Slide-and-exchange mechanism for rapid and selective transport through the nuclear pore complex. Proc. Natl. Acad. Sci. USA 2016, 113, E2489–E2497. [Google Scholar] [CrossRef] [Green Version]
  51. Paci, G.; Caria, J.; Lemke, E.A. Cargo transport through the nuclear pore complex at a glance. J. Cell Sci. 2021, 134, jcs247874. [Google Scholar] [CrossRef]
  52. Lennon, K.M.; Soheilypour, M.; Peyro, M.; Wakefield, D.L.; Choo, G.E.; Mofrad, M.R.K.; Jovanovic-Talisman, T. Characterizing binding interactions that are essential for selective transport through the nuclear pore complex. Int. J. Mol. Sci. 2021, 22, 10898. [Google Scholar] [CrossRef]
  53. Kutay, U.; Güttinger, S. Leucine-rich nuclear-export signals: Born to be weak. Trends Cell Biol. 2005, 15, 121–124. [Google Scholar] [CrossRef] [PubMed]
  54. Wing, C.E.; Fung, H.Y.J.; Chook, Y.M. Karyopherin-mediated nucleocytoplasmic transport. Nat. Rev. Mol. Cell. Biol. 2022. [Google Scholar] [CrossRef] [PubMed]
  55. Mohamed, M.S.; Hazawa, M.; Kobayashi, A.; Guillaud, L.; Watanabe-Nakayama, T.; Nakayama, M.; Wang, H.; Kodera, N.; Oshima, M.; Ando, T.; et al. Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core. Biomaterials 2020, 256, 120198. [Google Scholar] [CrossRef] [PubMed]
  56. Meier, I.; Brkljacic, J. The Arabidopsis nuclear pore and nuclear envelope. Arab. Book 2010, 8, e0139. [Google Scholar] [CrossRef] [Green Version]
  57. Gu, Y. The nuclear pore complex: A strategic platform for regulating cell signaling. New Phytol. 2018, 219, 25–30. [Google Scholar] [CrossRef] [PubMed]
  58. Parry, G. Assessing the function of the plant nuclear pore complex and the search for specificity. J. Exp. Bot. 2013, 64, 833–845. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  59. Ibarra, A.; Hetzer, M.W. Nuclear pore proteins and the control of genome functions. Genes Dev. 2015, 29, 337–349. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  60. Wiermer, M.; Cheng, Y.T.; Imkampe, J.; Li, M.; Wang, D.; Lipka, V.; Li, X. Putative members of the Arabidopsis Nup107-160 nuclear pore sub-complex contribute to pathogen defense. Plant J. 2012, 70, 796–808. [Google Scholar] [CrossRef]
  61. Du, J.; Gao, Y.; Zhan, Y.; Zhang, S.; Wu, Y.; Xiao, Y.; Zou, B.; He, K.; Gou, X.; Li, G.; et al. Nucleocytoplasmic trafficking is essential for BAK1- and BKK1-mediated cell-death control. Plant J. 2016, 85, 520–531. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Jia, M.; Shen, X.; Tang, Y.; Shi, X.; Gu, Y. A karyopherin constrains nuclear activity of the NLR protein SNC1 and is essential to prevent autoimmunity in Arabidopsis. Mol. Plant 2021, 14, 1733–1744. [Google Scholar] [CrossRef] [PubMed]
  63. Ludke, D.; Roth, C.; Kamrad, S.A.; Messerschmidt, J.; Hartken, D.; Appel, J.; Hornich, B.F.; Yan, Q.; Kusch, S.; Klenke, M.; et al. Functional requirement of the Arabidopsis importin-α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1. Plant J. 2021, 105, 994–1009. [Google Scholar] [CrossRef] [PubMed]
  64. Kim, H.; Shimura, H.; Sueda, K.; Masuta, C. Importin/exportin-mediated nucleocytoplasmic shuttling of cucumber mosaic virus 2b protein is required for 2b’s efficient suppression of RNA silencing. PLoS Pathog. 2022, 18, e1010267. [Google Scholar] [CrossRef]
  65. de Leone, M.J.; Hernando, C.E.; Mora-Garcia, S.; Yanovsky, M.J. It’s a matter of time: The role of transcriptional regulation in the circadian clock-pathogen crosstalk in plants. Transcription 2020, 11, 100–116. [Google Scholar] [CrossRef]
  66. Cheng, Y.T.; Germain, H.; Wiermer, M.; Bi, D.; Xu, F.; García, A.V.; Wirthmueller, L.; Després, C.; Parker, J.E.; Zhang, Y.; et al. Nuclear pore complex component MOS7/Nup88 is required for innate immunity and nuclear accumulation of defense regulators in Arabidopsis. Plant Cell 2009, 21, 2503–2516. [Google Scholar] [CrossRef] [Green Version]
  67. Xu, F.; Jia, M.; Li, X.; Tang, Y.; Jiang, K.; Bao, J.; Gu, Y. Exportin-4 coordinates nuclear shuttling of TOPLESS family transcription corepressors to regulate plant immunity. Plant Cell 2021, 33, 697–713. [Google Scholar] [CrossRef]
  68. Peng, S.; Guo, D.; Guo, Y.; Zhao, H.; Mei, J.; Han, Y.; Guan, R.; Wang, T.; Song, T.; Sun, K.; et al. Constitutive Expresser of Pathogenesis-Related Genes 5 is an RNA-binding protein controlling plant immunity via an RNA processing complex. Plant Cell 2022. [Google Scholar] [CrossRef] [PubMed]
  69. Huang, S.; Zhu, S.; Kumar, P.; MacMicking, J.D. A phase-separated nuclear GBPL circuit controls immunity in plants. Nature 2021, 594, 424–429. [Google Scholar] [CrossRef]
  70. Li, C.; Wang, J.; Ji, N.; Lei, C.; Wang, K. PpHOS1, a RING E3 ubiquitin ligase, interacts with PpWRKY22 in the BABA-induced priming defense of peach fruit against Rhizopus stolonifer. Postharvest. Biol. Technol. 2020, 159, 111029. [Google Scholar] [CrossRef]
  71. Imai, A.; Ohtani, M.; Nara, A.; Tsukakoshi, A.; Narita, A.; Hirakawa, H.; Sato, S.; Suganuma, N. The Lotus japonicus nucleoporin GLE1 is involved in symbiotic association with rhizobia. Physiol. Plant 2020, 168, 590–600. [Google Scholar] [CrossRef] [PubMed]
  72. Tamura, K.; Fukao, Y.; Hatsugai, N.; Katagiri, F.; Hara-Nishimura, I. Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana. Nucleus 2017, 8, 301–311. [Google Scholar] [CrossRef] [Green Version]
  73. Liu, C.; Xin, Y.; Xu, L.; Cai, Z.; Xue, Y.; Liu, Y.; Xie, D.; Liu, Y.; Qi, Y. Arabidopsis ARGONAUTE1 binds chromatin to promote gene transcription in response to hormones and stresses. Dev. Cell 2018, 44, 348–361.e7. [Google Scholar] [CrossRef] [Green Version]
  74. Song, X.; Li, Y.; Cao, X.; Qi, Y. MicroRNAs and their regulatory roles in plant-environment interactions. Annu. Rev. Plant Biol. 2019, 70, 489–525. [Google Scholar] [CrossRef] [PubMed]
  75. Wang, P.; Li, J.; Zhang, Z.; Zhang, Q.; Li, X.; Xiao, J.; Ma, H.; Wang, S. OsVQ1 links rice immunity and flowering via interaction with a mitogen-activated protein kinase OsMPK6. Plant Cell Rep. 2021, 40, 1989–1999. [Google Scholar] [CrossRef] [PubMed]
  76. Genenncher, B.; Wirthmueller, L.; Roth, C.; Klenke, M.; Ma, L.; Sharon, A.; Wiermer, M. Nucleoporin-regulated MAP kinase signaling in immunity to a necrotrophic fungal pathogen. Plant Physiol. 2016, 172, 1293–1305. [Google Scholar] [CrossRef] [Green Version]
  77. Rayapuram, N.; Jarad, M.; Alhoraibi, H.M.; Bigeard, J.; Abulfaraj, A.A.; Volz, R.; Mariappan, K.G.; Almeida-Trapp, M.; Schloffel, M.; Lastrucci, E.; et al. Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity. Proc. Natl. Acad. Sci. USA 2021, 118, e2004670118. [Google Scholar] [CrossRef]
  78. Tang, M.; Ning, Y.; Shu, X.; Dong, B.; Zhang, H.; Wu, D.; Wang, H.; Wang, G.L.; Zhou, B. The Nup98 homolog APIP12 targeted by the effector AvrPiz-t is involved in rice basal resistance against Magnaporthe oryzae. Rice 2017, 10, 5. [Google Scholar] [CrossRef] [Green Version]
  79. Li, C.; Liu, L.; Teo, Z.W.N.; Shen, L.; Yu, H. Nucleoporin 160 regulates flowering through anchoring HOS1 for destabilizing CO in Arabidopsis. Plant Commun. 2020, 1, 100033. [Google Scholar] [CrossRef]
  80. Cheng, Z.; Zhang, X.; Huang, P.; Huang, G.; Zhu, J.; Chen, F.; Miao, Y.; Liu, L.; Fu, Y.F.; Wang, X. Nup96 and HOS1 are mutually stabilized and gate CONSTANS protein level, conferring long-day photoperiodic flowering regulation in Arabidopsis. Plant Cell 2020, 32, 374–391. [Google Scholar] [CrossRef]
  81. Jiang, S.; Xiao, L.; Huang, P.; Cheng, Z.; Chen, F.; Miao, Y.; Fu, Y.F.; Chen, Q.; Zhang, X.M. Nucleoporin Nup98 participates in flowering regulation in a CONSTANS -independent mode. Plant Cell Rep. 2019, 38, 1263–1271. [Google Scholar] [CrossRef] [PubMed]
  82. Xiao, L.; Jiang, S.; Huang, P.; Chen, F.; Wang, X.; Cheng, Z.; Miao, Y.; Liu, L.; Searle, I.; Liu, C.; et al. Two nucleoporin98 homologous genes jointly participate in the regulation of starch degradation to repress senescence in Arabidopsis. BMC Plant Biol. 2020, 20, 292. [Google Scholar] [CrossRef]
  83. Gallemí, M.; Galstyan, A.; Paulišić, S.; Then, C.; Ferrández-Ayela, A.; Lorenzo-Orts, L.; Roig-Villanova, I.; Wang, X.; Micol, J.L.; Ponce, M.R.; et al. DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis. Development 2016, 143, 1623–1631. [Google Scholar] [PubMed] [Green Version]
  84. Xu, X.M.; Rose, A.; Muthuswamy, S.; Jeong, S.Y.; Venkatakrishnan, S.; Zhao, Q.; Meier, I. NUCLEAR PORE ANCHOR, the Arabidopsis homolog of Tpr/Mlp1/Mlp2/megator, is involved in mRNA export and SUMO homeostasis and affects diverse aspects of plant development. Plant Cell 2007, 19, 1537–1548. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  85. Van Bergen, N.J.; Bell, K.M.; Carey, K.; Gear, R.; Massey, S.; Murrell, E.K.; Gallacher, L.; Pope, K.; Lockhart, P.J.; Kornberg, A.; et al. Pathogenic variants in nucleoporin TPR (translocated promoter region, nuclear basket protein) cause severe intellectual disability in humans. Hum. Mol. Genet. 2022, 31, 362–375. [Google Scholar] [CrossRef]
  86. Kosar, M.; Giannattasio, M.; Piccini, D.; Maya-Mendoza, A.; Garcia-Benitez, F.; Bartkova, J.; Barroso, S.I.; Gaillard, H.; Martini, E.; Restuccia, U.; et al. The human nucleoporin Tpr protects cells from rna-mediated replication stress. Nat. Commun. 2021, 12, 3937. [Google Scholar] [CrossRef] [PubMed]
  87. Lainé, J.P.; Singh, B.N.; Krishnamurthy, S.; Hampsey, M. A physiological role for gene loops in yeast. Genes Dev. 2009, 23, 2604–2609. [Google Scholar] [CrossRef] [Green Version]
  88. Tan-Wong, S.M.; Wijayatilake, H.D.; Proudfoot, N.J. Gene loops function to maintain transcriptional memory through interaction with the nuclear pore complex. Genes Dev. 2009, 23, 2610–2624. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  89. Lee, K.; Seo, P.J. The Arabidopsis E3 ubiquitin ligase HOS1 contributes to auxin biosynthesis in the control of hypocotyl elongation. Plant Growth Regul. 2015, 76, 157–165. [Google Scholar] [CrossRef]
  90. Kim, J.H.; Lee, H.J.; Jung, J.H.; Lee, S.; Park, C.M. HOS1 facilitates the phytochrome B-mediated inhibition of PIF4 function during hypocotyl growth in Arabidopsis. Mol. Plant 2017, 10, 274–284. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  91. Kim, J.H.; Lee, H.J.; Park, C.M. HOS1 acts as a key modulator of hypocotyl photomorphogenesis. Plant Signal Behav. 2017, 12, e1315497. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  92. Kemp, C.; Coleman, A.; Wells, G.; Parry, G. Overexpressing components of the nuclear transport apparatus causes severe growth symptoms in tobacco leaves. Plant Signal Behav. 2015, 10, e1000103. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  93. Bao, S.; Shen, G.; Li, G.; Liu, Z.; Arif, M.; Wei, Q.; Men, S. The Arabidopsis nucleoporin Nup1 is essential for megasporogenesis and early stages of pollen development. Plant Cell Rep. 2019, 38, 59–74. [Google Scholar] [CrossRef] [PubMed]
  94. Bi, X.; Cheng, Y.J.; Hu, B.; Ma, X.; Wu, R.; Wang, J.W.; Liu, C. Nonrandom domain organization of the Arabidopsis genome at the nuclear periphery. Genome Res. 2017, 27, 1162–1173. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  95. Park, G.T.; Frost, J.M.; Park, J.S.; Kim, T.H.; Lee, J.S.; Oh, S.A.; Twell, D.; Brooks, J.S.; Fischer, R.L.; Choi, Y. Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis. Proc. Natl. Acad. Sci. USA 2014, 111, 18393–18398. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  96. Braud, C.; Zheng, W.; Xiao, W. LONO1 encoding a nucleoporin is required for embryogenesis and seed viability in Arabidopsis. Plant Physiol. 2012, 160, 823–836. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  97. Boeglin, M.; Fuglsang, A.T.; Luu, D.T.; Sentenac, H.; Gaillard, I.; Chérel, I. Reduced expression of atNUP62 nucleoporin gene affects auxin response in Arabidopsis. BMC Plant Biol. 2016, 16, 2. [Google Scholar] [CrossRef] [Green Version]
  98. Ferrández-Ayela, A.; Alonso-Peral, M.M.; Sánchez-García, A.B.; Micol-Ponce, R.; Pérez-Pérez, J.M.; Micol, J.L.; Ponce, M.R. Arabidopsis TRANSCURVATA1 encodes NUP58, a component of the nucleopore central channel. PLoS ONE 2013, 8, e67661. [Google Scholar] [CrossRef] [Green Version]
  99. Zhu, Y.; Wang, B.; Tang, K.; Hsu, C.C.; Xie, S.; Du, H.; Yang, Y.; Tao, W.A.; Zhu, J.K. An Arabidopsis nucleoporin Nup85 modulates plant responses to ABA and salt stress. PLoS Genet. 2017, 13, e1007124. [Google Scholar] [CrossRef] [Green Version]
  100. Chen, J.; Yu, Y.; Sui, X.; Qiao, L.; Dong, C.H. Arabidopsis CPR5 regulates ethylene signaling via interacting with ETR1 N-terminus and controlling mRNAs nucleocytoplasmic transport. bioRxiv 2019. [Google Scholar] [CrossRef] [Green Version]
  101. Qiao, H.; Shen, Z.; Huang, S.S.; Schmitz, R.J.; Urich, M.A.; Briggs, S.P.; Ecker, J.R. Processing and subcellular trafficking of ER-tethered EIN2 control response to ethylene gas. Science 2012, 338, 390–393. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  102. Wen, X.; Zhang, C.; Ji, Y.; Zhao, Q.; He, W.; An, F.; Jiang, L.; Guo, H. Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus. Cell Res. 2012, 22, 1613–1616. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  103. Allen, J.R.; Strader, L.C. Nucleocytoplasmic partitioning as a mechanism to regulate Arabidopsis signaling events. Curr. Opin. Cell Biol. 2021, 69, 136–141. [Google Scholar] [CrossRef] [PubMed]
  104. Zhu, T.; Herrfurth, C.; Xin, M.; Savchenko, T.; Feussner, I.; Goossens, A.; De Smet, I. Warm temperature triggers JOX and ST2A-mediated jasmonate catabolism to promote plant growth. Nat. Commun. 2021, 12, 4804. [Google Scholar] [CrossRef] [PubMed]
  105. Ye, K.; Li, H.; Ding, Y.; Shi, Y.; Song, C.; Gong, Z.; Yang, S. BRASSINOSTEROID-INSENSITIVE2 negatively regulates the stability of transcription factor ICE1 in response to cold stress in Arabidopsis. Plant Cell 2019, 31, 2682–2696. [Google Scholar] [CrossRef] [PubMed]
  106. Zhang, Y.; Wang, Y.; Liu, J.; Ding, Y.; Wang, S.; Zhang, X.; Liu, Y.; Yang, S. Temperature-dependent autoimmunity mediated by chs1 requires its neighboring TNL gene SOC3. New Phytol. 2017, 213, 1330–1345. [Google Scholar] [CrossRef]
  107. Dong, C.H.; Hu, X.; Tang, W.; Zheng, X.; Kim, Y.S.; Lee, B.H.; Zhu, J.K. A putative Arabidopsis nucleoporin, AtNup160, is critical for RNA export and required for plant tolerance to cold stress. Mol. Cell. Biol. 2006, 26, 9533–9543. [Google Scholar] [CrossRef] [Green Version]
  108. Zhang, A.; Wang, S.; Kim, J.; Yan, J.; Yan, X.; Pang, Q.; Hua, J. Nuclear pore complex components have temperature-influenced roles in plant growth and immunity. Plant Cell Environ. 2020, 43, 1452–1466. [Google Scholar] [CrossRef]
  109. Karimi, M.; Shiran, B.; Rabei, M.; Fallahi, H.; Eri, B.B. A study of Arabidopsis cold stress tolerance improvement via AthHOS1-targeting HOS1-amiRNA approach. Res. Sq. 2021. [Google Scholar] [CrossRef]
  110. Dong, C.H.; Agarwal, M.; Zhang, Y.; Xie, Q.; Zhu, J.K. The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1. Proc. Natl. Acad. Sci. USA 2006, 103, 8281–8286. [Google Scholar] [CrossRef] [Green Version]
  111. Shkryl, Y.; Yugay, Y.; Avramenko, T.; Grigorchuk, V.; Gorpenchenko, T.; Grischenko, O.; Bulgakov, V. CRISPR/Cas9-mediated knockout of HOS1 reveals its role in the regulation of secondary metabolism in Arabidopsis thaliana. Plants 2021, 10, 104. [Google Scholar] [CrossRef] [PubMed]
  112. Lazaro, A.; Mouriz, A.; Piñeiro, M.; Jarillo, J.A. Red light-mediated degradation of CONSTANS by the E3 ubiquitin ligase HOS1 regulates photoperiodic flowering in Arabidopsis. Plant Cell 2015, 27, 2437–2454. [Google Scholar] [CrossRef] [Green Version]
  113. Ding, Y.; Li, H.; Zhang, X.; Xie, Q.; Gong, Z.; Yang, S. OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis. Dev. Cell 2015, 32, 278–289. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  114. Jung, J.H.; Park, J.H.; Lee, S.; To, T.K.; Kim, J.M.; Seki, M.; Park, C.M. The cold signaling attenuator HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 activates FLOWERING LOCUS C transcription via chromatin remodeling under short-term cold stress in Arabidopsis. Plant Cell 2013, 25, 4378–4390. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  115. Jung, J.H.; Lee, H.J.; Park, M.J.; Park, C.M. Beyond ubiquitination: Proteolytic and nonproteolytic roles of HOS1. Trends Plant Sci. 2014, 19, 538–545. [Google Scholar] [CrossRef] [PubMed]
  116. MacGregor, D.R.; Gould, P.; Foreman, J.; Griffiths, J.; Bird, S.; Page, R.; Stewart, K.; Steel, G.; Young, J.; Paszkiewicz, K.; et al. HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 is required for circadian periodicity through the promotion of nucleo-cytoplasmic mRNA export in Arabidopsis. Plant Cell 2013, 25, 4391–4404. [Google Scholar] [CrossRef] [Green Version]
  117. Han, S.H.; Park, Y.J.; Park, C.M. HOS1 activates DNA repair systems to enhance plant thermotolerance. Nat. Plants 2020, 6, 1439–1446. [Google Scholar] [CrossRef] [PubMed]
  118. Zhang, C.; An, N.; Jia, P.; Zhang, W.; Xing, L. MdNup62 interactions with MdHSFs involved in flowering and heat-stress tolerance in apple. Authorea 2021. [Google Scholar] [CrossRef]
  119. Ge, Y.F.; Han, J.Y.; Zhou, G.X.; Xu, Y.M.; Ding, Y.; Shi, M.; Guo, C.K.; Wu, G. Silencing of miR156 confers enhanced resistance to brown planthopper in rice. Planta 2018, 248, 813–826. [Google Scholar] [CrossRef] [PubMed]
  120. Guo, C.K.; Xu, Y.M.; Shi, M.; Lai, Y.M.; Wu, X.; Wang, H.S.; Zhu, Z.J.; Poethig, R.S.; Wu, G. Repression of miR156 by miR159 regulates the timing of the juvenile-to-adult transition in Arabidopsis. Plant Cell 2017, 29, 1293–1304. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  121. Zhao, J.; Shi, M.; Yu, J.; Guo, C.K. SPL9 mediates freezing tolerance by directly regulating the expression of CBF2 in Arabidopsis thaliana. BMC Plant Biol. 2022, 22, 59. [Google Scholar] [CrossRef] [PubMed]
Ijms 23 03031 g001 550
Figure 1. A model for the plant NPC structural organization and the role of plant NUPs in different biological processes. The NPCs consist of the following main components: scaffold nucleoporins include outer ring (blue), inner ring (yellow), the transmembrane ring nucleoporins (green), linker nucleoporins (purple), cytoplasmic filaments (dark) and nuclear basket (red), and central FG nucleoporins (dark purple). NUPs that have been functionally investigated in plants are underlined; and the left part lists some plant NUPs that have been reported to be involved in immune signaling (blue triangle), growth and development (green star), hormone response (purple diamond), and temperature response (orange cycle).
Figure 1. A model for the plant NPC structural organization and the role of plant NUPs in different biological processes. The NPCs consist of the following main components: scaffold nucleoporins include outer ring (blue), inner ring (yellow), the transmembrane ring nucleoporins (green), linker nucleoporins (purple), cytoplasmic filaments (dark) and nuclear basket (red), and central FG nucleoporins (dark purple). NUPs that have been functionally investigated in plants are underlined; and the left part lists some plant NUPs that have been reported to be involved in immune signaling (blue triangle), growth and development (green star), hormone response (purple diamond), and temperature response (orange cycle).
Ijms 23 03031 g001
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Wu, X.; Han, J.; Guo, C. Function of Nuclear Pore Complexes in Regulation of Plant Defense Signaling. Int. J. Mol. Sci. 2022, 23, 3031. https://doi.org/10.3390/ijms23063031

AMA Style

Wu X, Han J, Guo C. Function of Nuclear Pore Complexes in Regulation of Plant Defense Signaling. International Journal of Molecular Sciences. 2022; 23(6):3031. https://doi.org/10.3390/ijms23063031

Chicago/Turabian Style

Wu, Xi, Junyou Han, and Changkui Guo. 2022. "Function of Nuclear Pore Complexes in Regulation of Plant Defense Signaling" International Journal of Molecular Sciences 23, no. 6: 3031. https://doi.org/10.3390/ijms23063031

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop