Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines
Abstract
:1. Introduction
2. Results
2.1. Morphological Analysis of the Microtubule System Revealed a Difference in Microtubule Architecture between the Two Lines of Green Monkey Kidney Cells
2.2. The Centrosome and the Golgi Have Different Impacts on Microtubule Organization in the Vero and BS-C-1 Cells
2.3. Different Nucleation Activity at the Golgi Membranes in the Vero and BS-C-1 Cells Coexists with the Same Activity of Their Centrosomes
2.4. CAMSAP3 Overexpression Alters Microtubule Organization in the Golgi-Independent Manner
2.5. The GCC185-CLASP2 Axis Is Not Determining for the Golgi Activity as an MTOC
2.6. Transcriptomic Analysis Reveals Several Hitherto Unknown Potential Factors That May Influence the Golgi Functioning as a Microtubule Organizer
3. Discussion
4. Materials and Methods
4.1. Cultured Cells, Transfection, Inhibitors, Microtubule Regrowth, Cytoplast Preparation, Fixation
4.2. Antibodies
4.3. Immunofluorescent Staining and Microscopy, Live Imaging and Data Analysis
4.4. DNA Constructs
4.5. SDS-PAGE and Immunoblotting
4.6. RNA Sequencing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Alternative Name | BS-C-1 | Vero | BS-C-1/Vero |
---|---|---|---|---|
Reads | Reads | |||
AKAP9 | AKAP450 | 1754 | 1871 | 0.94 |
CAMSAP1 | 1215 | 1737 | 0.7 | |
CAMSAP2 | 2338 | 1909 | 1.22 | |
CAMSAP3 | 200 | 54 | 3.75 | |
CDK5RAP1 | 574 | 866 | 0.66 | |
CDK5RAP2 | 747 | 880 | 0.85 | |
CDK5RAP3 | 1194 | 1669 | 0.72 | |
CEP350 | 1584 | 1552 | 1.02 | |
CLASP1 | 1224 | 1038 | 1.18 | |
CLASP2 | 1276 | 1338 | 0.95 | |
CLASRP | 300 | 530 | 0.57 | |
GCC2 | GCC185 | 1529 | 1056 | 1.45 |
GOLGA2 | GM130 | 1181 | 989 | 1.19 |
GORASP1 | GRASP65 | 1678 | 940 | 1.79 |
MMG * | LOC103247162 | 0 | 0 | 0 |
LOC103224073 | 5 | 2 | 2.5 | |
LOC103224075 | 0.5 | 0.5 | 1 | |
MTCL1 | CCDC165 | 1413 | 871 | 1.62 |
NIN | ninein | 1610 | 1539 | 1.05 |
TRIP11 | GMAP210 | 1062 | 1366 | 0.78 |
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Brodsky, I.B.; Fokin, A.I.; Efremov, A.A.; Nadezhdina, E.S.; Burakov, A.V. Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines. Int. J. Mol. Sci. 2022, 23, 16178. https://doi.org/10.3390/ijms232416178
Brodsky IB, Fokin AI, Efremov AA, Nadezhdina ES, Burakov AV. Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines. International Journal of Molecular Sciences. 2022; 23(24):16178. https://doi.org/10.3390/ijms232416178
Chicago/Turabian StyleBrodsky, Ilya B., Artem I. Fokin, Aleksei A. Efremov, Elena S. Nadezhdina, and Anton V. Burakov. 2022. "Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines" International Journal of Molecular Sciences 23, no. 24: 16178. https://doi.org/10.3390/ijms232416178