Overexpression of CfICE1 from Cryptomeria fortunei Enhances Cold, Drought and Salt Stress in Poplar
Abstract
:1. Introduction
2. Results
2.1. CfICE1 Overexpression Caused Morphological Changes in Transgenic Poplars
2.2. Physiological Response of CfICE1 Transgenic Poplar Plants to Cold Stress
2.3. Physiological Response of CfICE1 Transgenic Poplar Plants to Salt Stress
2.4. Physiological Response of CfICE1 Transgenic Poplar Plants to Drought Stress
2.5. Ultrastructure of Poplar Leaves under Different Stresses
2.6. Analysis of DAB Staining and H2O2 Content in Poplar Leaves under Different Stresses
2.7. Expression of CfICE1 and PdbCBF in WT and CfICE1-Overexpressing Poplar under Different Stresses
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. DNA Extraction, RNA Extraction and cDNA Synthesis
4.3. Genetic Transformation in Poplar
4.4. Physiological Index Analysis of Plants Treated with Cold, Salt and Drought
4.5. Transmission Electron Microscopy (TEM) Analysis
4.6. DAB Staining and Determination of the H2O2 Content
4.7. Semiquantitative PCR and Real-Time Quantification PCR
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ICE1 , | inducer of CBF expression 1; |
CBF, | C-repeat binding factor; |
COR, | cold-regulated gene; |
bHLH, | basic helix–loop–helix; |
WT, | wild-type; |
ORF, | open reading frame; |
OD, | optical density; |
EL, | electrolyte leakage; |
DAB, | diaminobenzidine; |
H2O2, | hydrogen peroxide; |
SOD, | superoxide dismutase; |
POD, | peroxidase; |
MDA, | malondialdehyde; |
ROS, | reactive oxygen species. |
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Types | WT | 35S::pBI121 | 35S::CfICE1-1 | 35S::CfICE1-2 | 35S::CfICE1-3 |
---|---|---|---|---|---|
Height (cm) | 15.22 ± 0.91 | 14.56 ± 0.42 | 11.61 ± 0.55 ** | 13.61 ± 0.55 * | 11.67 ± 0.85 ** |
Length (cm) | 4.39 ± 0.31 | 3.90 ± 0.45 | 3.51 ± 0.36 | 3.78 ± 0.47 | 3.54 ± 0.41 |
Width (cm) | 3.57 ± 0.06 | 3.03 ± 0.57 | 2.79 ± 0.38 | 3.02 ± 0.55 | 2.80 ± 0.38 |
Length/Width | 1.23 ± 0.07 | 1.31 ± 0.13 | 1.26 ± 0.04 | 1.26 ± 0.08 | 1.27 ± 0.1 |
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Zhu, L.; Yang, J.; Zhang, Y.; Hu, H.; Cui, J.; Xue, J.; Xu, J. Overexpression of CfICE1 from Cryptomeria fortunei Enhances Cold, Drought and Salt Stress in Poplar. Int. J. Mol. Sci. 2022, 23, 15214. https://doi.org/10.3390/ijms232315214
Zhu L, Yang J, Zhang Y, Hu H, Cui J, Xue J, Xu J. Overexpression of CfICE1 from Cryptomeria fortunei Enhances Cold, Drought and Salt Stress in Poplar. International Journal of Molecular Sciences. 2022; 23(23):15214. https://doi.org/10.3390/ijms232315214
Chicago/Turabian StyleZhu, Lijuan, Junjie Yang, Yingting Zhang, Hailiang Hu, Jiebing Cui, Jinyu Xue, and Jin Xu. 2022. "Overexpression of CfICE1 from Cryptomeria fortunei Enhances Cold, Drought and Salt Stress in Poplar" International Journal of Molecular Sciences 23, no. 23: 15214. https://doi.org/10.3390/ijms232315214