Platelet Reactivity and Inflammatory Phenotype Induced by Full-Length Spike SARS-CoV-2 Protein and Its RBD Domain
Abstract
:1. Introduction
2. Results
2.1. SARS-CoV-2 Spike Full-Length Protein and RBD Domain Induce Platelet Activation
2.2. Full-Length Spike Promotes Platelet Aggregation in the Presence of Low Doses of Collagen
2.3. The Spike Protein and Its RBD Domain Stimulates Platelets’ Release of Pro-Inflammatory Factors
2.4. Platelets Contribute to a Procoagulant Microenvironment when Stimulated with Full Length Spike Protein and RBD Domain
3. Discussion
4. Materials and Methods
4.1. SARS-CoV-2 Spike Full-Length and RBD Domain Proteins
4.2. Blood Sample Collection
4.3. Platelet Rich Plasma Isolation
4.4. Platelet Stimulation with Spike SARS-CoV-2 and RBD Domain
4.5. Flow Cytometry Assays
4.6. Determination of Immunothrombotic Biomarkers in Platelet Stimulated Supernatant
4.7. Light Transmission Aggregation Assays
4.8. Statement of Ethics
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cano-Mendez, A.; García-Larragoiti, N.; Damian-Vazquez, M.; Guzman-Cancino, P.; Lopez-Castaneda, S.; Ochoa-Zarzosa, A.; Viveros-Sandoval, M.E. Platelet Reactivity and Inflammatory Phenotype Induced by Full-Length Spike SARS-CoV-2 Protein and Its RBD Domain. Int. J. Mol. Sci. 2022, 23, 15191. https://doi.org/10.3390/ijms232315191
Cano-Mendez A, García-Larragoiti N, Damian-Vazquez M, Guzman-Cancino P, Lopez-Castaneda S, Ochoa-Zarzosa A, Viveros-Sandoval ME. Platelet Reactivity and Inflammatory Phenotype Induced by Full-Length Spike SARS-CoV-2 Protein and Its RBD Domain. International Journal of Molecular Sciences. 2022; 23(23):15191. https://doi.org/10.3390/ijms232315191
Chicago/Turabian StyleCano-Mendez, Alan, Nallely García-Larragoiti, Maria Damian-Vazquez, Patricia Guzman-Cancino, Sandra Lopez-Castaneda, Alejandra Ochoa-Zarzosa, and Martha Eva Viveros-Sandoval. 2022. "Platelet Reactivity and Inflammatory Phenotype Induced by Full-Length Spike SARS-CoV-2 Protein and Its RBD Domain" International Journal of Molecular Sciences 23, no. 23: 15191. https://doi.org/10.3390/ijms232315191