An Assessment of Metallothionein–Cadmium Binding in Rat Uterus after Subchronic Exposure Using a Long–Term Observation Model
Abstract
:1. Introduction
2. Results
2.1. Cd and MT
2.2. Determination of Trace Elements (Cu, Zn, Ca and Mg) in Uterus Tissue
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Experimental Protocol
Euthanasia, Tissue Collection, Preservation and Mineralization
4.3. Cd, Zn, Mg, Ca, Cu Assessment
4.4. MT Determinations
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | Dose [mg/kg b.w.] | T-Cd [ng/g] | MT-Cd [ng/g] | nonMT-Cd [ng/g] |
---|---|---|---|---|
Group A (90-Day Exposure) | ||||
Pure control | 0 | 29.24 ±10.11 | 19.65 ± 1.91 | 9.59 ± 1.02 |
Cd | 0.09 | 37.48 ±12.41 | 20.60 ± 9.49 | 16.48 ± 6.94 |
Cd | 0.9 | 145.82 ± 11.41 a | 32.28 ± 4.46 a | 118.81 ± 6.88 a |
Cd | 1.8 | 685.63 ± 35.90 a | 35.90 ± 10.25 a | 645.23 ± 35.40 a |
Cd | 4.5 | 1021.6 ± 76.36 a | 50.76 ± 2.99 a | 968.20 ± 75.99 a |
Group B (90-day exposure and 90-day post-exposure period) | ||||
Pure control | 0 | 28.03 ± 6.81 | 16.94 ± 4.32 | 10.89 ± 2.01 |
Cd | 0.09 | 48.64 ± 10.21 a | 19.38 ± 4.79 a | 29.21 ± 6.31 a |
Cd | 0.9 | 153.97 ± 31.16 a | 44.31 ± 3.03 a | 109.97 ± 30.18 a |
Cd | 1.8 | 487.57 ± 50.92 a | 45.51 ± 3.48 a | 439.98 ± 50.06 a |
Cd | 4.5 | 945.78 ± 16.83 a | 69.47 ± 6.69 a | 875.53 ± 15.43 a |
Group C (90-day exposure and 180-day post-exposure period) | ||||
Pure control | 0 | 30.34 ± 1.89 | 20.45 ± 1.71 | 9.89 ± 1.12 |
Cd | 0.09 | 38.95 ± 13.09 | 23.84 ± 9.76 | 15.18 ± 12.98 |
Cd | 0.9 | 148.68 ± 28.33 a | 55.44 ± 5.98 a | 93.32 ± 28.01 a |
Cd | 1.8 | 452.64 ± 95.26 a | 67.45 ± 1.39 a | 385.49 ± 94.83 a |
Cd | 4.5 | 896.23 ± 21.33 a | 101.02 ± 17.81 a | 795.62 ± 20.14 a |
Group (n = 40) | Period | Subgroup (n = 8) | Dose (mg/kg b.w.) | Determinations in Uterus |
---|---|---|---|---|
A | 90-day of exposure | Control | water | T-Cd, Cd-MT and nonCd-MT (calculated) Essential elements: Zn, Cu, Ca, Mg |
Cd | 0.09 | |||
Cd | 0.9 | |||
Cd | 1.8 | |||
Cd | 4.5 | |||
B | 90-day of exposure + 90-day of observation | Control | water | T-Cd, Cd-MT and nonCd-MT (calculated) Essential elements: Zn, Cu, Ca, Mg |
Cd | 0.09 | |||
Cd | 0.9 | |||
Cd | 1.8 | |||
Cd | 4.5 | |||
C | 90-day of exposure + 180-day of observation | Control | water | T-Cd, Cd-MT and nonCd-MT (calculated) Essential elements: Zn, Cu, Ca, Mg |
Cd | 0.09 | |||
Cd | 0.9 | |||
Cd | 1.8 | |||
Cd | 4.5 |
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Nasiadek, M.; Stragierowicz, J.; Kilanowicz, A. An Assessment of Metallothionein–Cadmium Binding in Rat Uterus after Subchronic Exposure Using a Long–Term Observation Model. Int. J. Mol. Sci. 2022, 23, 15154. https://doi.org/10.3390/ijms232315154
Nasiadek M, Stragierowicz J, Kilanowicz A. An Assessment of Metallothionein–Cadmium Binding in Rat Uterus after Subchronic Exposure Using a Long–Term Observation Model. International Journal of Molecular Sciences. 2022; 23(23):15154. https://doi.org/10.3390/ijms232315154
Chicago/Turabian StyleNasiadek, Marzenna, Joanna Stragierowicz, and Anna Kilanowicz. 2022. "An Assessment of Metallothionein–Cadmium Binding in Rat Uterus after Subchronic Exposure Using a Long–Term Observation Model" International Journal of Molecular Sciences 23, no. 23: 15154. https://doi.org/10.3390/ijms232315154