Human Milk Oligosaccharide 2′-Fucosyllactose Inhibits Ligand Binding to C-Type Lectin DC-SIGN but Not to Langerin
Abstract
:1. Introduction
2. Results
2.1. c2′-FL, p2′-FL, s2′-FL and HMOs Inhibit the Ligand Binding to DC-SIGN, but Not to Langerin
2.2. c2′-FL, p2′-FL, and s2′-FL Inhibit the Binding of Leb to the DC-SIGN Expressing Cells
2.3. Molecular Dynamic Simulation Suggested That 2′-FL Exists in Bioactive Conformation to Bind DC-SIGN but Not Langerin
2.4. Leb Interacts with DC-SIGN with Two Fucoses Operating One at a Time: Consequences for the Displacement by 2′-FL
3. Discussion
4. Materials and Methods
4.1. Antibodies and Beads
4.2. Cells
4.3. HMOs
4.4. Purification of c2′-FL to Obtain p2′-FL
4.5. Ligand-Receptor Competition Assay
4.6. Fluorescent Bead Adhesion Cell-Ligand Competition Assay
4.7. Molecular Dynamics Simulation
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Mukherjee, R.; Somovilla, V.J.; Chiodo, F.; Bruijns, S.; Pieters, R.J.; Garssen, J.; van Kooyk, Y.; Kraneveld, A.D.; van Bergenhenegouwen, J. Human Milk Oligosaccharide 2′-Fucosyllactose Inhibits Ligand Binding to C-Type Lectin DC-SIGN but Not to Langerin. Int. J. Mol. Sci. 2022, 23, 14745. https://doi.org/10.3390/ijms232314745
Mukherjee R, Somovilla VJ, Chiodo F, Bruijns S, Pieters RJ, Garssen J, van Kooyk Y, Kraneveld AD, van Bergenhenegouwen J. Human Milk Oligosaccharide 2′-Fucosyllactose Inhibits Ligand Binding to C-Type Lectin DC-SIGN but Not to Langerin. International Journal of Molecular Sciences. 2022; 23(23):14745. https://doi.org/10.3390/ijms232314745
Chicago/Turabian StyleMukherjee, Reshmi, Victor J. Somovilla, Fabrizio Chiodo, Sven Bruijns, Roland J. Pieters, Johan Garssen, Yvette van Kooyk, Aletta D. Kraneveld, and Jeroen van Bergenhenegouwen. 2022. "Human Milk Oligosaccharide 2′-Fucosyllactose Inhibits Ligand Binding to C-Type Lectin DC-SIGN but Not to Langerin" International Journal of Molecular Sciences 23, no. 23: 14745. https://doi.org/10.3390/ijms232314745