Tear Proteome Revealed Association of S100A Family Proteins and Mesothelin with Thrombosis in Elderly Patients with Retinal Vein Occlusion
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. General Workflow of the Study
4.2. Subjects and Ethical Consideration
4.3. Sample Collection and Preparation
4.4. Liquid Chromatography and High-Resolution Mass Spectrometry Analysis
4.5. Proteomic Data Analysis
4.6. Statistical Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Identifiers | Frequency | Concentration, fmoles/ng | FC log2 | p-Value | ||||
---|---|---|---|---|---|---|---|---|
Accession | Gene | Name | CRVO | Control | CRVO | Control | ||
P61769 | B2M | β2-microglobulin | 0.91 | 0.9 | 2.29 ± 1.72 | 10.04 ± 10.34 | −2.13 | 1.49 × 10−10 |
P60709 | ACTB | Actin cytoplasmic 1 | 1 | 1 | 5.73 ± 8.81 | 14.06 ± 8.17 | −1.3 | 4.67 × 10−3 |
P01859 | IGHG2 | Immunoglobulin heavy γ2 | 1 | 1 | 6.37 ± 11.42 | 14.12 ± 12.43 | −1.15 | 7.62 × 10−4 |
Q96DA0 | ZG16B | Zymogen granule protein 16 homolog B | 0.83 | 0.72 | 1.29 ± 0.87 | 2.6 ± 3.26 | −1.01 | 3.86 × 10−3 |
P01860 | IGHG3 | Immunoglobulin heavy γ3 | 1 | 0.97 | 0.56 ± 0.87 | 1 ± 1.63 | −0.83 | 2.20 × 10−2 |
P02790 | HPX | Hemopexin | 0.91 | 0.97 | 2.48 ± 2.58 | 3.52 ± 4.71 | −0.51 | 1.23 × 10−2 |
A0A0A0MRZ7 | IGKV2D−26 | Immunoglobulin κ-variable 2D-26 | 0.61 | 0.76 | 1.7 ± 0.68 | 2.32 ± 1.86 | −0.45 | 2.14 × 10−2 |
P0DOY3 | IGLC3 | Immunoglobulin λ3 | 1 | 1 | 27.37 ± 17.28 | 34.2 ± 20.03 | −0.32 | 1.12 × 10−2 |
P01591 | JCHAIN | Immunoglobulin J chain | 1 | 1 | 11.03 ± 8.44 | 10.97 ± 15.47 | 0.01 | 4.00 × 10−2 |
P04792 | HSPB1 | Heat shock protein β1 | 0.96 | 0.97 | 1.62 ± 2.87 | 1.53 ± 1.22 | 0.08 | 2.13 × 10−2 |
P10909 | CLU | Clusterin | 1 | 0.97 | 5.07 ± 4.28 | 4.37 ± 4.94 | 0.21 | 9.48 × 10−3 |
P02647 | APOA1 | Apolipoprotein A-I | 1 | 0.97 | 5.57 ± 12.16 | 4.18 ± 7.12 | 0.42 | 3.93 × 10−2 |
Q16378 | PRR4 | Proline-rich protein 4 | 0.65 | 0.76 | 41.4 ± 40.03 | 30.01 ± 25.78 | 0.46 | 1.22 × 10−2 |
P22079 | LPO | Lactoperoxidase | 0.74 | 0.66 | 0.73 ± 0.66 | 0.52 ± 0.29 | 0.47 | 1.61 × 10−2 |
P0C0L4 | C4A | Complement C4-A | 0.61 | 0.69 | 0.81 ± 0.42 | 0.57 ± 0.58 | 0.52 | 1.93 × 10−3 |
P01034 | CST3 | Cystatin-C | 0.91 | 0.62 | 1.22 ± 0.9 | 0.81 ± 1.1 | 0.58 | 8.46 × 10−3 |
P19652 | ORM2 | α1-acid glycoprotein 2 | 1 | 0.93 | 1.64 ± 1.69 | 1.09 ± 1.4 | 0.59 | 3.97 × 10−2 |
P01023 | A2M | α2-macroglobulin | 0.65 | 0.83 | 1.54 ± 1.12 | 0.99 ± 0.97 | 0.64 | 1.32 × 10−2 |
Q9UGM3 | DMBT1 | Deleted in malignant brain tumors 1 protein | 0.83 | 0.97 | 11.41 ± 11.35 | 7.18 ± 6.87 | 0.67 | 1.82 × 10−3 |
P02787 | TF | Serotransferrin | 1 | 1 | 13.91 ± 14.94 | 8.73 ± 10.61 | 0.67 | 2.56 × 10−2 |
P01036 | CST4 | Cystatin-S | 1 | 0.97 | 22.82 ± 10.7 | 13.24 ± 12.76 | 0.79 | 1.61 × 10−3 |
P25311 | AZGP1 | Zinc-α2-glycoprotein | 1 | 1 | 49.07 ± 10.71 | 28.26 ± 18.61 | 0.8 | 2.02 × 10−7 |
Q13421 | MSLN | Mesothelin | 0.78 | 0.83 | 0.95 ± 0.59 | 0.54 ± 0.44 | 0.82 | 1.44 × 10−4 |
P06703 | S100A6 | Protein S100-A6 | 1 | 0.9 | 8.62 ± 11.01 | 3.99 ± 2.28 | 1.11 | 6.00 × 10−5 |
Q99935 | OPRPN | Opiorphin prepropeptide | 1 | 0.97 | 49.38 ± 25.83 | 22.82 ± 29.03 | 1.11 | 5.92 × 10−4 |
P02763 | ORM1 | α1-acid glycoprotein 1 | 1 | 0.97 | 7.68 ± 9.04 | 3.41 ± 5.3 | 1.17 | 3.19 × 10−2 |
P02766 | TTR | Transthyretin | 0.91 | 0.52 | 0.81 ± 0.78 | 0.28 ± 0.26 | 1.54 | 8.87 × 10−6 |
P06702 | S100A9 | Protein S100-A9 | 1 | 0.9 | 8.78 ± 17.08 | 2.07 ± 1.08 | 2.08 | 9.50 × 10−14 |
P05109 | S100A8 | Protein S100-A8 | 1 | 0.86 | 3.74 ± 7.48 | 0.68 ± 0.62 | 2.45 | 1.75 × 10−12 |
Parameter | CRVO Group | Control Group | p-Value | ||
---|---|---|---|---|---|
Population size | 28 | 29 | - | ||
Gender ratio (male/female) | 15/13 (53%/47%) | 17/12 (58%/42%) | 0.80 ‡ | ||
Diagnosis | OD | OS | OU | - | - |
Incomplete occlusion | 4 | 2 | - | ||
Occlusion (Thrombosis) | 10 | 10 | 2 | ||
Age, average (±SD), years old | 71.7 ± 5.6 | 75.1 ± 7.1 | 0.47 # | ||
Myocardial ischemia | 10 | 6 | - | ||
Diabetes mellitus (type 2) | 2 | - | |||
Hypertensive disease | 26 | 27 | |||
Anticoagulant medication | 11 | - | |||
Central retinal thickness, µm † | 282 ± 167 | 157 ± 39 | |||
Total cholesterol level, mmol/L | |||||
less than 5 mmol/L | 4 (14%) | 29 | - | ||
5–7 mmol/L | 17 (61%) | - | |||
more than 7 mmol/L | 7 (25%) | - |
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Stepanov, A.; Usharova, S.A.; Malsagova, K.A.; Moshetova, L.K.; Turkina, K.I.; Kopylov, A.T.; Kaysheva, A.L. Tear Proteome Revealed Association of S100A Family Proteins and Mesothelin with Thrombosis in Elderly Patients with Retinal Vein Occlusion. Int. J. Mol. Sci. 2022, 23, 14653. https://doi.org/10.3390/ijms232314653
Stepanov A, Usharova SA, Malsagova KA, Moshetova LK, Turkina KI, Kopylov AT, Kaysheva AL. Tear Proteome Revealed Association of S100A Family Proteins and Mesothelin with Thrombosis in Elderly Patients with Retinal Vein Occlusion. International Journal of Molecular Sciences. 2022; 23(23):14653. https://doi.org/10.3390/ijms232314653
Chicago/Turabian StyleStepanov, Alexander, Svetlana A. Usharova, Kristina A. Malsagova, Larisa K. Moshetova, Ksenia I. Turkina, Arthur T. Kopylov, and Anna L. Kaysheva. 2022. "Tear Proteome Revealed Association of S100A Family Proteins and Mesothelin with Thrombosis in Elderly Patients with Retinal Vein Occlusion" International Journal of Molecular Sciences 23, no. 23: 14653. https://doi.org/10.3390/ijms232314653